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We narrowed to 54 results for: guide rna expression plasmid

Showing: 21 - 40 of 54 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression, Ligation Independent ...Empty mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag ...TEV cleavage Transient expression - Gradia Lab plasmids for bacterial expression, Ligation Independent ... Expression - Rubin lab plasmids for Drosophila expression pMT-puro - Inducible...C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 ... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...

  2. Rett Syndrome

    Type
    Collection
    ... Jaenisch labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the ...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional... editing, MECP2 reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...information on generating iPSCs using plasmids, please see Addgene's Plasmids for Stem Cell Research page. CRISPR... search bar or sort buttons to find plasmids based on: Expression system (mammalian or bacterial) Insert...human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External Resources Research...

  3. Antibody Guide

    Type
    Collection
    ... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies. Antibodies.... Recombinant expression validation A recombinant expression strategy is used to express the protein of...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic...produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary in... comparison of expression between different samples, by normalizing protein expression to the controls...In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and...

  4. Microbiology Resources

    Type
    Collection
    ... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic...resources, or read our guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species...Biology Assembly Standards Guide Bacterial Expression Systems : Find plasmids to perform your experiment...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica...

  5. Lentivirus Plasmids

    Type
    Collection
    ... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging ... Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR... VSV-G-expressing envelope plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid. Didier...CRISPR gRNA? Browse our catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid Generation...destination plasmid for cDNA expression. Miguel Ramalho-Santos 24129 pULTRA 3rd Bicistronic expression of EGFP... 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dTomato version...Ecotropic MLV expressing envelope plasmid. Arthur Nienhuis and Patrick Salmon 17532 pLTR-G Expression of VSV-...

  6. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Lentiviral Vector Guide and Adenoviral Vector Guide Find more plasmids in our Lentiviral Plasmids and Adenoviral...Adenoviral Plasmids collections Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Content...reporter plasmids to test whether or not you've efficiently generated infectious virus, plasmids expressing...-EF1-DIO-copGFP 72253 Expresses copGFP under EF-1 promoter when Cre is expressed by another vector pCDH-EF1...pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter when Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro...pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP and iCre under from the CB promoter pCDH-CB-iCre 72257 Express iCre under ...pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter pCDH-EF1-Fon-copGFP 72260 Expresses copGFP from...

  7. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid...Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression Renilla...

  8. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...activation plasmids. To learn more about CRISPR activation and other CRISPR topics, read our CRISPR Guide . Mammalian...ID Plasmid Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter...C. elegans ID Plasmid Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  9. CRISPR References and Information

    Type
    Collection
    ...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR... CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression: pAC2 , ...129 KB Sontheimer gRNA design and cloning All-in-one plasmid containing expression cassette for NmCas9 and...597 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330)... for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription...endogenous proteins pFETCh_Donor ; additional HDR & gRNA plasmids are available for CREB1, ATF1, GABPA, and RAD21... generation; gRNA design; HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180...

  10. Chemogenetics Plasmids

    Type
    Collection
    ...Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection Chemogenetics plasmids encode genetically engineered...Biosensors Fluorescent Proteins Guide Optogenetics Plasmids Optogenetics Guide Blog: Chemogenetics Blog: Chemogenetics...experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search this table ...ready-to-use AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Find plasmids for chemogenetic experiments, including popular DREADDs and PSAMs. Plasmid... Plasmid Collections ...

  11. Worm Expression Resources

    Type
    Collection
    ... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ... Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain a worm...'s worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous...backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...sequence: ID Plasmid Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the...

  12. CRISPR Plasmids - Parasites

    Type
    Collection
    ...protein-coding genes. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...some of the plasmids that have been used in these efforts. Addgene's parasite CRISPR plasmid collection...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... gondii genome-wide knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding...CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA...

  13. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA...CRISPR plasmids for transcriptional repression of target genes. CRISPR... CRISPR Interfere CRISPR Plasmids: Interfere...

  14. Fluorescent Protein Guide

    Type
    Collection
    ... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that are...Resources Addgene's plasmid repository contains thousands of fluorescent proteins. Use this guide to learn more...proteins and to find plasmids for your next experiment. Browse Fluorescent Protein Plasmids by Category Empty...Additional Resources at Addgene Optogenetics Guide | Optogenetics Plasmids Use light to activate, inhibit, and ...molecular pathways or cells. Chemogenetics Guide | Chemogenetics Plasmids Use small molecules to activate or .... Luciferase Plasmid Collection Use bioluminescent reporters for studying gene expression, signaling pathways...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering...Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description...-inducible expression. pTAL6-BB contains the TEF1 promoter, giving constitutive expression of TALORs built... For both plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting...Maria-Elena Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors...pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...

  16. COVID-19 Resources

    Type
    Collection
    ... Geiss Plasmids SARS-CoV-2 Plasmids Browse the tables below to find popular plasmids expressing genes ... Plasmid Collections COVID-19 – Plasmids and Resources COVID-19 and Coronavirus Plasmids & Resources...Antibodies SARS-CoV-2 Plasmids Other Coronavirus Plasmids Mammalian Targets General Plasmid Tools Pooled Libraries...cytokines and related plasmids see our Immunology Resource Guide . Return to top General Plasmid Tools Browse ...versions designed for your expression system. Some of the following plasmids are available to industry,...for-profit entity, please see our Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional... This page includes a table of all spike expression plasmids in our collection that are suitable for generating...

  17. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...fusing a biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system...

  18. CRISPR Plasmids - Plants

    Type
    Collection
    ...following CRISPR plasmids have been designed for use in plants. To find emtpy gRNA plasmids, visit Addgene's...Addgene's Empty gRNA Expression Vectors page and search the table for "Plant". Activate ID Plasmid Gene/Insert...CRISPR expression plasmids that have been designed for use in plants. CRISPR...Edit ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/...Interfere ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Nick ID Plasmid Gene/...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  19. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach...CRISPR Cas3 plasmids. To learn more about Cas3 and other CRISPR topics, read our CRISPR Guide . Mammalian...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...

  20. Plasmid Collections

    Type
    Collection
    ...Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains 166,785 plasmids . We've...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression...you are looking for a non-plasmid item, browse our entire repository . Plasmid Resources Our curated resources...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated...Curated Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion... Expression Kits Kits are groups of plasmids that have been packaged together because they are intended...
Showing: 21 - 40 of 54 results