...Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful...fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...

...Cas proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs). Through sequence...guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine...check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene...CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . 32(3):279-84. PMID: 24463574...Weissman JS, Qi LS. 2013. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell... JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity... M, DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science . ...

...Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such ...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have..., nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with...Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection none, need Cas9 plasmid Calarco...

...Reporter Plasmids You may also like… Plasmids 101 Addgene's Molecular Biology Guides Plasmids 101: E. ...CRISPR plasmids, see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA ...Controlled Expression Reporter Plasmids Reporter Plasmids Tagging and Visualization Purification Controlled Expression...or staining, and RNA transcript tags to monitor RNA localization. Using these plasmids you’ll hopefully... on expression only at a specific time. This collection has a variety of plasmids whose expression levels...interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids Various Plasmids pBAD, OR2...drive gene expression at a specific level. Return to Top Reporter Plasmids Reporter plasmids can be used...

...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...tables below for CRISPR RNA targeting plasmids. Plasmids are available for expression in mammalian systems...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR... target RNA rather than DNA. In bacteria, once they have recognized and cleaved the target RNA sequence...to attentuate RNA levels. In mammalian systems, Cas13a does not exhibit the collateral RNA degradation ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...

...γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral...10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro...retroviral plasmid Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See... additional plasmids in this toolkit. Lowe 64865 pLncEXP CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc...Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression in mammalian ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR...Purify Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

...Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Validated In Resistance Co-expressed Cas9 Depositing...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression...CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have...sites. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select... Select a gRNA expression plasmid based on factors such as selectable marker or cloning method. When ...

...structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems target RNA, not DNA, across...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...variety of factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...Bacteria CRISPR plasmids designed for use in bacteria. C. elegans CRISPR plasmids designed for use in ...Drosophila CRISPR plasmids designed for use in Drosophila . Mammalian CRISPR plasmids designed for use ...

...Addgene plasmid ID 42230). This plasmid allows for the simultaneous expression of sgRNA and SpCas9, but... multiple sgRNAs may be expressed from a single plasmid. Add “CACC” before the 20-mer guide sequence and...cellular delivery of a pair of chimeric single guide RNAs (sgRNAs) to create two double strand breaks (DSBs...Cell Lines Other Addgene Resources CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present...cloning and expression purposes. NOTE: Here we discuss the usage of the pSpCas9(BB) plasmid (pX330) (Addgene...utilized, such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP ... with a PCR cloning kit) into a plasmid vector. Transform the plasmid into DH5α E. coli cells and plate...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...below for CRISPR Cascade-Cas3 plasmids. Plasmids are available for expression in mammalian systems, bacteria...the Cascade complex to express equally. To combat this, some CRISPR-Cas3 plasmids take an all-in-one approach...plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...tables below for CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

... protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens in ...Return to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516...Bacterial Protein Expression Vectors BioBricks Foundation Plasmids Drew Endy Lab Plasmids Jennifer Molloy...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification...Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via the links below:...Reverse Transcriptases RNA Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4...165516 pOpen-SP6rpol SP6 RNA Polymerase 165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes...

...electroporation Transient expression of CRISPR components Expression decreases as RNA is degraded within the...multi-plasmid SAM system. Select Expression System To use CRISPR, you will need both Cas9 and a gRNA expressed...details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian...lentivirus, see our Lentivirus Guide ) Stable, tunable expression of Cas9 and/or gRNA in a wide variety of mammalian...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing both ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...below for CRISPR base editing plasmids. Plasmids are available for expression in mammalian systems, bacteria...CRISPR Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...tables below for CRISPR nickase plasmids. Plasmids are available for expression in mammalian systems, bacteria...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR...CRISPR nickase plasmids for the generation of single-strand breaks. CRISPR...

...ways. Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting... a list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...biological processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes... of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...function, see our CRISPR plasmids resource page . Cas9 for Editing Genomic Sequences To perform genome edits...52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...

...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...using any of these gRNA plasmids and review the publication associated with each plasmid for more information...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA plasmids...gRNA expression vectors available from Addgene. CRISPR...

...Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize...below for CRISPR plasmids designed to introduce a DSB. Plasmids are available for expression in mammalian ...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert... elegans ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert...Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Xenopus ID Plasmid Gene/Insert...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for the expression of ...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...