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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 137 results for: ica

Showing: 21 - 40 of 137 results

  1. University of Florida Serotype Testing Panel for the Eye and Brain

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    Collection
    ... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite...Strategies for Overcoming Donor-Variation and Implications in Genome Editing. Sci Rep . Oct 19;6:35495....

  2. AAV for Neuronal Tracing

    Type
    Collection
    ...cell, and specifically targets the starter cell via the EnvA receptor. RABV is able to replicate in the ...which is not required for the transcription or replication of the genome within infected cells, but is required...present, the virus can spread from this cell to synaptically connected cells (meaning, it can spread to cells... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...complementation, G-deleted rabies virus can spread to monosynaptically connected cells. To further refine the targeting... was modified so that it could only infect a genetically specified neuronal population. This is done using...leukosis virus. EnvA can direct virus infection specifically into cells that express the TVA viral receptor...

  3. Neurodegeneration Research Collection

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    Collection
    ...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority...’s disease (PD) is a chronic and progressive neurological disorder that primarily affects movement. More... consists mainly of managing symptoms through medication and surgery. PD primarily involves the malfunction...available from Addgene, sortable by type of genetic modification and target species. You can also find our guide...Sun et al. Nat Methods. 2020 Oct 21. Glutamate indicators with improved activation kinetics and localization... transcription factors for sensory, motor, and cortical neuron differentiation. Held et al. Cell Rep. ...A multicolor atlas for whole-brain neuronal identification in C. elegans. Yemini et al. Cell. 2021 Jan...

  4. Distribution to Industry

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    Collection
    ...activity in a variety of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don't See What You're Looking For? Please...within 1 business day regarding your account verification. Once your account has been verified, you will.... Pricing Addgene is a nonprofit organization dedicated to providing the scientific community with open...access to plasmid research tools. Pricing will be indicated on the product pages after you login to your account...depositing institution(s). It governs the transfer of biological materials and intellectual property rights, and...

  5. AAV Packaged on Request

    Type
    Collection
    ...implementation letter DNA amplification Viral vector production Density centrifugation purification Addgene’s comprehensive...preps, which includes MTA facilitation, DNA amplification, and high-quality quality control measures such...preparations, we set both minimum standards and typical titers, though actual yields may vary by construct...construct. Size Quantity Total Volume Minimum Titer Typical Titer Small NEW 2 × 100 µL aliquots 0.2 mL 4 × ... with a minimum titer of 2 × 10 12 GC/mL and a typical titer of 7 × 10 12 GC/mL. For more details, see...remain subject to the terms and conditions of the applicable transfer plasmid. We will help you gain legal...providing scientist through an MTA. This process typically takes 1–4 days, depending on your institution....

  6. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9 fused to a transcriptional activator...

  7. Caltech Systemic Capsids

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    ...vector preparations were produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB...vector preparations were produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available...vector preparations were produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847) . Browse Available...vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS1 plasmid (Addgene #185136) . Browse Available...vector preparations were produced with the pUCmini-iCAP-AAV.MaCPNS2 plasmid (Addgene #185137) . Browse Available...vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid (Addgene #175004) . Browse Available...vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid (Addgene #175005) . Browse Available...

  8. Fluorescent Protein Guide

    Type
    Collection
    ...small biomolecules or other physiological intracellular processes with genetically encoded fluorescent biosensors...mammals. Regulate Biological Activity Use fluorescent proteins to modulate biological activity, like transcription...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Use this guide to learn more about the many applications of fluorescent proteins (FPs) and to find the...Plasmids | Guide Use small molecules to activate genetically engineered cellular receptors that affect signalling...Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed ...

  9. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...and are thus well suited to directed evolution applications. Examples of these base editing systems include...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

  10. Tetracycline Inducible Expression

    Type
    Collection
    ...tTA-Advanced Takeshi Imai Other Tet Applications Explore some other popular applications of tetracycline systems ...powerful tools to control gene expression and study biological targets. The tetracycline (tet) system is one...improvements. A synthetic Tet Response Element (TRE) typically features seven copies of tet O upstream of a minimal... to a reverse Tet repressor, or rTetR, which specifically binds the TRE in the presence of tetracycline...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated...pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid #35626 as a reporter...

  11. Viral Vector Guides and Plasmids

    Type
    Collection
    ...strong immunogenic response, useful for clinical applications as vaccines. Selecting a Viral Vector Type...vectors are popular for making stable cell lines or clinical use. Adenoviral Vector Guide | Adenoviral Plasmids... research goals. For example, AAV vectors are typically preferred for in vivo studies because of their...used to transduce cells of interest. Specific modifications to these plasmids, such as different promoters...been carefully engineered for safety and are replication-incompetent. Specific genes have been mutated...mutated or deleted to prevent uncontrolled viral replication, and the required genes are divided in the multi-plasmid...viral genome. Therefore, viruses used in research typically do not have the ability to propagate in the same...

  12. Microbiology Resources

    Type
    Collection
    ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...distributes cannot be used to reconstitute self-replicating microbes or recreate the diseases they cause....our Search page to look for specific genes or applications that are not listed here. Addgene Microbiology...Prions Plasmids for Protozoa Species Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii...vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly...transcription units in the oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors...fluorescent protein plasmid collection is organized by application and by color. External Resources European Saccharomyces...

  13. Plan Your Experiment

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    ...with most experiments, you first need to have a biological question in mind. Then you can decide how to ...component for each category. Genetic Manipulation Application Cas Enzyme Additional Considerations Browse Plasmids...gene you target will depend on your specific application. Knockout : Target constitutively expressed regions...protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression can...systems and delivery methods . Validate Genetic Modification Once you have successfully delivered the gRNA...questions: Does the resulting population need to be identical (clonal) or is a mixed population okay? Does the...the edit need to be homozygous? What type of modification is it? Are you modifying the genomic sequence...

  14. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... by Talley Lambert at Harvard Medical School Zeiss Microscopy : Optical Highlighters NIC Wiki PSFP : Nikon...545-51 Shcherbakova et al. : Journal of the American Chemical Society, May 2012, Vol. 134 No.18, pp. 7913...Nature Communications, November 2012, Vol. 3 No. 1204 Wiedenmann et al. : Journal of Biomedical Optics...Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively by life... proteins created by Talley Lambert at Harvard Medical School More References below Jump to: Blue/UV Cyan... tagging with a wide variety of colors, some specifically optimized for yeast Lindquist Lab Vectors - ... Vol. 11 No. 5, pp.572-8 Yu et al. : Nature Communications, May 2014 Vol. 15 No. 5, p. 3626 Do you have...

  15. Antibody Plasmid Collection

    Type
    Collection
    ...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene...and time. An alternative approach is to use synthetically produced recombinant antibodies, created by ...extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb...imaging. Learn more about antibodies and their applications in our Antibody Guide ! Cloning and Expressing...antibodies, and Fc-silent mutants. Functional diversification of hybridoma-produced antibodies by CRISPR/...456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents...and Generation of synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger...

  16. Promega Plasmid Collection

    Type
    Collection
    ...synthetic chemical ligands, which have been developed for special functions that vary from purification, visualization...Promega technologies that support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT...useful for both intracellular and extracellular applications where you want minimal disruption of endogenous...levels in lysates or live-cells, thus preserving physiological relevance. NanoBiT Assays NanoLuc Binary Technology...similar premise, but examine the proximity of a chemical compound and its target protein. The apparent ...Connections (Link opens in a new window) blogs for technical tips and tools for genetic reporter assays (Link...

  17. Retrograde AAV viral preps

    Type
    Collection
    ...55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases Deisseroth...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases Deisseroth...AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f and physically separate nuclear dTomato Calcium sensor Ting ...-DIO-GCaMP6f-P2A-nls-dTomato EF1a GCaMP6f and physically separate nuclear dTomato, Cre-dependent Calcium...AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s and physically separate nuclear dTomato Calcium sensor Ting ...sensor Looger 113050 pAAV-hSyn-GRAB_DA1h Syn genetically-encoded fluorescent dopamine(DA) sensor GRAB_...Dopamine sensor Li 123308 pAAV-hSyn-GRAB_NE1m Syn genetically-encoded fluorescent norepinephrine(NE) sensor...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ... nuclease along with the guide RNA. For most applications, we recommend using Cas9 with the single guide...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into the vector...BbsI, and a pair of annealed oligos (design is indicated below) can be cloned into the CRISPR array. The...- sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene... genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating...function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo , the Zhang Lab established...can be combined with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin...

  19. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Plant ID Plasmid ...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional...

  20. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID ...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...
Showing: 21 - 40 of 137 results