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Showing: 41 - 60 of 128 results

  1. Distribution to Industry

    Type
    Collection
    ...Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards...PDB-deposited), disulfide-free, mesophilic, and designed to be diverse in topology, size, and isoelectric...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...Parkinson's Disease; For target validation and drug design Antibodies Addgene distributes ready-to-use recombinant...materials and intellectual property rights, and provides liability protection for material providers. Addgene's...

  2. Promega Plasmid Collection

    Type
    Collection
    ...Promega.com. ID Plasmid Description Industry Plasmid Technology Overview This collection includes specialized Promega...contains plasmids developed by Promega scientists and designed to streamline molecular and cellular biology experimental...support a range of applications. The technology includes NanoLuc, HaloTag, SmBiT, LgBiT, HiBiT, and more... a new window) is an energy transfer technique designed to measure molecular proximity in living cells...

  3. TALEN Guide

    Type
    Collection
    ...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays ...that came out in the spring of 2011, the groups describe a set of customized plasmids that can be used ... a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August, uses a serial...in Nature Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in...effector and TALEN kits here . References Efficient design and assembly of custom TALEN and other TAL effector-based...

  4. Lentiviral Prep Service

    Type
    Collection
    ... Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout pooled...2A-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library Version...Version 1 of the Celltag library contains 19973 barcodes to combinatorially index cells for single-cell...Version 2 of the Celltag library contains 4934 barcodes to combinatorially index cells for single-cell...Version 3 of the Celltag library contains 5737 barcodes to combinatorially index cells for single-cell...

  5. CRISPR Plasmids - Yeast

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in yeast....Resources The following CRISPR plasmids have been designed for use in yeast. To find emtpy gRNA plasmids,...PI Publication Return to top Nick ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  6. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...CRISPR plasmids based on Cas13 designed to precisely edit RNA targets....mutation reduces off-target effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-...or search the tables below for CRISPR plasmids designed for RNA editing. To learn more about RNA editing... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  7. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the ...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at...mcherry with either 0, 11, 18, or 20 matching nucleotides. Together, these plasmids allow to explore the...

  8. Chemogenetics Plasmids

    Type
    Collection
    ... chemogenetic receptors: Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) and Pharmacologically...opto-chemogenetic actuator. For more detailed descriptions of chemogenetic receptors, ligands, and how ...preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for...

  9. Adenovirus Plasmids

    Type
    Collection
    ...recombination with the shuttle plasmid. This collection provides a curated selection of popular plasmids that can...for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production of viruses containing...adenoviral vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use ... for adenoviral vector production. ID Strain Description PI 16398 BJ5183 E. coli strain for recombination...

  10. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...138152 pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector expressing destabilized EGFP...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...neutralizing activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral ...

  11. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...after the promoter, and so on. The following table provides information for the assembly standards most commonly...will need to be reverse complimented for primer design. The restriction enzymes used for assembly are ..., NotI , PstI Scar: GCTAGT Features: 6 bp scar encodes Ala-Ser; Allows for in-frame fusions; NheI is rare...Enzymes: BamHI , Xhol Scar: GGATCT Features: Scar encodes Gly-Ser in-frame with prefix start codon; Employs...

  12. Retrovirus Plasmids

    Type
    Collection
    ...contain a viral genome encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...Gamma-Retroviral Packaging Plasmids ID Plasmid Description PI 8449 pUMVC Contains MoMLV gag and pol . Use...Gamma-Retroviral Envelope Plasmids ID Plasmid Description PI 1733 pCI-VSVG VSV-G recommended for the FELIX...

  13. Bacterial Expression Systems

    Type
    Collection
    ... cleavage sites. Other protein tags and signal peptides are used to enhance protein solubility and to ...Commonly used tags, cleavage sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep II, ...protease, factor Xa, enterokinase, thrombin Signal peptides for periplasmic localization: PelB, MalE, OmpA...Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic...a collection of CRISPR plasmids that have been designed for their use in bacteria in our Bacterial CRISPR...

  14. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...required for AAV viral replication. This collection provides a curated selection of transfer, packaging, and... packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging...for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid,...repository for AAV vector production. ID Plasmid Description PI Return to top Other Resources Addgene's Viral...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ... experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link...

  16. Optogenetics AAV Preps

    Type
    Collection
    ... (soma-targeted) mRuby2 Cre dependent 9 Hillel Adesnik 124603 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40... (soma-targeted) mRuby3 Cre dependent 9 Hillel Adesnik 170163 pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3 ...pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3 CAG ChroME2s mRuby3 Cre dependent 1 Hillel Adesnik 124651 pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 CaMKII... (soma-targeted) mRuby3 Cre dependent 9 Hillel Adesnik 137155 pAAV-nEF-Con/Fon-iC++-EYFP nEF iC++ EYFP...

  17. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in Xenopus....Resources The following CRISPR plasmids have been designed for use in Xenopus. To find emtpy gRNA plasmids... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  18. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in zebrafish....Resources The following CRISPR plasmids have been designed for use in zebrafish. To find emtpy gRNA plasmids... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  19. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in C. elegans....Resources The following CRISPR plasmids have been designed for use in C. elegans. To find emtpy gRNA plasmids... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

  20. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in Drosophila and other insects....Resources The following CRISPR plasmids have been designed for use in Drosophila and other insects. To find... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...
Showing: 41 - 60 of 128 results