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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 154 results for: TOR;

Showing: 41 - 60 of 154 results

  1. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Expression Vectors You can use the tables on Addgene's Empty gRNA Vectors page to search based on factors such...specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  2. CRISPR References and Information

    Type
    Collection
    ... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...webtool that uses deep learning models for base editor gRNA design and efficiency prediction. Developed...webtool that uses deep learning models for prime editor pegRNA design and efficiency prediction. Developed... Lab and maintained by the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder...information from Cas-OFFinder and Microhomology predictor. Developed by the Jin-Soo Kim Lab . CRISPR-ERA...

  3. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout...ligand-binding domain such as the human estrogen receptor (ERT2) so that the enzyme is only activated in...you can do with it, read Addgene's blog on FLEx Vectors . Flp-IN: A single FRT site within the genome of...to minimize leakiness in experiments using AAV vectors. Highlighted Plasmids Browse the tables below to...Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Explore...and filter the results by expression system and vector type. Find in-stock viral preps expressing Cre ...Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources Search...

  4. Synthetic Biology - Overview

    Type
    Collection
    ...and synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also... Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches...cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium Community Resources...including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic...Plasmids and Phagemids Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins...Plasmid Kit Gray Lab Maize and Rice Transcription Factors Hasty Lab Biopixels Jaramillo Lab Riboregulation...Foundation Registry of Standard Biological Parts - Repository of community-created BioBricks JBEI - Joint Bio-Energy...

  5. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...to an acceptor. Since the transfer of energy does not occur by emission of a photon, the acceptor molecule...and measuring the emission of the acceptor. For a given donor-acceptor pair, the FRET efficiency strongly...constructed using the cpFRET kit from the Pertz laboratory. Empty Vectors Encoding Fluorescent Proteins for FRET...proteins have been developed for use as donor and acceptor pairs in FRET experiments. The efficiency of ... depends on the distance between the donor and acceptor molecules and can therefore be used to calculate...calculate the distances between the donor and acceptor. FRET is often used to study (1) protein-protein interactions...each protein is separately fused to a donor or acceptor molecule (also referred to as intermolecular or...

  6. TALEN Engineering

    Type
    Collection
    ...Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith... developed three platforms for engineering TAL effector repeat arrays of any desired length: the REAL ...2012). All three of these platforms assemble TAL effector repeat arrays on a single architectural framework...all three methods are identical in sequence. TAL effector repeat arrays produced on this framework has yielded..., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013...

  7. Antibody Guide

    Type
    Collection
    ...antibodies can be stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C... Antibody Handling Storage Check the manufacturer’s instructions for exact storage requirements. As a ...tips for storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °... antibodies should not be stored in sodium azide, as it acts as an inhibitor to HRP. In those cases, thimerosal...define key terms, summarize antibody production and storage techniques, and explain common applications. Science...expensive than polyclonal antibodies. Environmental factors, binding partners, location in the cell, and sample... a primary antibody, which becomes the limiting factor for the number of proteins you can detect in a ...

  8. Zebrafish Plasmid Collection

    Type
    Collection
    .... pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS),...encode zebrafish receptors or monoclonal antibodies to zebrafish cell surface receptors and secreted proteins...found below: UFlip CRISPR targeted integration vectors for cell-type specific conditional gene inactivation...inactivation - Jeffrey Essner Lab GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner...mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish...zebrafish Whole organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure...with four promoters, six fluorophores, and empty vectors designed to streamline the production of transgenic...

  9. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Two classes of base editors — cytosine base editors (CBEs) and adenine base editors (ABEs) — can be used...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ... without double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically...dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus by a gRNA, and...on the opposite strand). Likewise, adenine base editors have been engineered to convert adenosine to inosine...Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further...

  10. CRISPR Plasmids - Yeast

    Type
    Collection
    ...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  11. AAV Molecular Tools

    Type
    Collection
    ...Molecular Tools Viral Vector Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification...encoding tet-off transactivators and tools for affinity purification (TRAP). Viral... Viral Vector Packaging Service...Engineering Labeling Overexpression Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible...-inducible tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible...CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible... promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...

  12. Microbiology Resources

    Type
    Collection
    ...a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and distributions...Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial...plasmid tools for the community that aren't in the repository? Help us widen our resources for the community...protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration...Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription units in ...lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion proteins, etc -...

  13. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  14. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...tevopreq1-GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian...Find prime editor plasmids for (search and replace) genome editing. CRISPR...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...enzyme is named PE1 . PE1 and most other prime editors use the Moloney murine leukemia virus (M-MLV) reverse...can be used in PE2–PE5 systems PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition...addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA -...

  15. CRISPR Plasmids - Plants

    Type
    Collection
    ...cytosine base editor that can alter DNA bases without inducing a DNA break. Cytosine base editors convert C...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such as selectable marker...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by...Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert...

  16. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...support educators interested in integrating MDH-related research into their teaching laboratories. They ...access to mentors, virtual and local professional development, membership to a local hub of educators teaching... Depositor Collections MDH CUREs Community Malate Dehydrogenase...They encourage instructors teaching different types of courses, at any type of institution, and at different...teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional hub (Link opens in a ...a new window) and contact the hub director for more information. Why MDH? MDH is stable, inexpensive to...cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of ...

  17. Chemogenetics AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics...information about this field. Receptor hM3D(Gq) DREADD rM3D(Gs) DREADD Inhibitory hM4D(Gi) DREADD KOR DREADD...Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion...AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Browse all Chemogenetics AAV Don’t See ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

  18. Antibody Plasmid Collection

    Type
    Collection
    ...reduced immune effector functions Expression of IgG Monoclonals with Engineered Immune Effector Functions....light-chain DNAs into a single mammalian expression vector A tool kit for rapid cloning and expression of ...1827:313-334. Daniel Christ pComb3 phage display vector system for producing human antibodies Synthetic...Carlos Barbas Variant of the pComb3 phage display vector system for isolation of scFv, diabody and Fab fragment...Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection...antibody fragments in the form of scFvs A simple vector system to improve performance and utilisation of...monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount ...

  19. COVID-19 Resources

    Type
    Collection
    ...cellular receptors. The cellular receptor for SARS-CoV-2 and SARS-CoV is hACE2, an angiotensin receptor. Another...Angiotensin Converting Enzyme 2) is the host cell receptor mediating the entry of SARS-CoV and SARS-CoV-2...Cytokines - SARS-CoV-2 infection induces a cytokine storm response in the body. For a full list of cytokines...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397... mutants (Timothy Whitehead). SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning Library ...comprehensive mapping (Jesse Bloom). SARS-CoV-2 Spike Receptor Binding Domain Site-Saturation Mutagenesis Libraries...and Is Blocked by a Clinically Proven Protease Inhibitor . FURIN - Andersen, KG, et al, 2020, Nature Medicine...

  20. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ... Depositor Collections Allen Institute for Brain Science ...Enhancer AAV plasmids are plasmids which use genomic regulatory sequences — termed enhancers — to drive cell ... AAV delivery. The collection includes the top vectors with the highest specificity and brightness, assessed...pAAV-AiE2563m-minBG-SYFP2-WPRE3-BGHpA AiP1969 AiE2563m SYFP2 Entorhinal area - L2 pyramidal neurons Hippocampal formation...pAAV-AiE2403m-minBG-SYFP2-WPRE3-BGHpA AiP1772 AiE2403m SYFP2 Entorhinal area - L2 pyramidal neurons Hippocampal formation...pAAV-AiE2664m-minBG-SYFP2-WPRE3-BGHpA AiP2107 AiE2664m SYFP2 Entorhinal area - L3 pyramidal neurons Hippocampal formation...pAAV-AiE2664m-minBG-iCre(R297T)-BGHpA AiP20169 AiE2664m Cre Entorhinal area - L3 pyramidal neurons Hippocampal formation...
Showing: 41 - 60 of 154 results