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Showing: 41 - 60 of 61 results

  1. CRISPR References and Information

    Type
    Collection
    ...Zhang GeCKO pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast...Link opens in a new window) An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...Labs. ACEofBASEs (Link opens in a new window) Identification of sgRNA target sites and off-target evaluation...number of potential off-targets, target's genomic location, and genome annotation. Available genomes are ...sequence, which can be an accession number, genomic location, pasted nucleotide sequence, or a sequence text... Target Finder (Link opens in a new window) Identification of gRNA target sequences from an input sequence...window) A collection of protocols, materials, and publications by members of the Division of Signaling and ...

  2. E11 Bio PRISM Collection

    Type
    Collection
    ...optical imaging down to individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled...components can be used separately for additional applications. Park & Sheridan et al. (2025) use plasmids ...circuit mapping and reconstruction. Potential applications could also include using individual or subsets...

  3. AAV for Neuronal Tracing

    Type
    Collection
    ... and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein....which is not required for the transcription or replication of the genome within infected cells, but is required... cells that lack the G protein, the virus can replicate but the newly formed viruses are unable to infect...starter cell via the EnvA receptor. RABV is able to replicate in the starter cell using the G-protein, which...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...

  4. Institute for Protein Innovation

    Type
    Collection
    ... proteins are currently under development. Figure 2: Key adhesion proteins and receptor–ligand pairs bridge...Antibody Description Isotype Reactivity Recommended Applications PI Browse Glypican Antibodies Glypicans are ...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Synaptic Cleft IPI’s Synaptic...Antibody Description Isotype Reactivity Recommended Applications PI Return to top Integrins Integrins are a family...Antibody Description Isotype Reactivity Recommended Applications Return to top Browse Epitope Tag Antibodies ...Antibody Collection page to browse Addgene’s full catalog of antibodies that target epitope tags and other...Antibody Description Isotype Reactivity Recommended Applications Return to top Browse Antibody Plasmids The following...

  5. Distribution to Industry

    Type
    Collection
    ...and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from ...activity in a variety of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don't See What You're Looking For? Please...within 1 business day regarding your account verification. Once your account has been verified, you will.... Pricing Addgene is a nonprofit organization dedicated to providing the scientific community with open...access to plasmid research tools. Pricing will be indicated on the product pages after you login to your account...

  6. Antibody Production

    Type
    Collection
    ...Each antibody catalog item is tested for shipping stability by incubating at 37 °C for 2 weeks, freezing...recommended applications. The specific QC experiments performed varies for each antibody catalog item. Whenever...specific antibody can be found on the antibody's catalog page or by contacting [email protected] . Some of...Innovation (IPI). For these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage....cells and will interfere with conjugation. For applications such as these, the buffer can be removed by ...

  7. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient...compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents) ACP...formation in nonribosomal peptide biosynthesis. Catalytic role of the condensation domain. J Biol Chem. ...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion...bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission...advantages and disadvantages depending on the application, and different luciferases require different ... course experiments, but is too dim for some applications. NanoLuc® is small (19 kDa), very bright, and... available, making it ideal for a variety of applications. To learn more and plan your experiment, read...OgNLuc ) fusions for BRET-based imaging and other applications. And see PalmGRET , a membrane-anchored version...

  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ...dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...delivering additional recombinases 10–14 . Figure 2: INTRSECT works by inserting short, intronic sequences...inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...8661 p4455 FLAG-hPLIC-2 UBQLN2 Flag CMV ALS Peter Howley 8662 p4456 FLAG-hPLIC-2 NTF UBQLN2 Flag CMV ALS...196208 GB1-A11(2-196)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196209 GB1-A11(2-196, G38R)-Strep...196210 GB1-A11(2-196, D40G)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196211 GB1-A11(2-196, G175R...196212 GB1-A11(2-196, G189E)-Strep ANXA11 His, Strep, Tev T7 ALS Lalit Deshmukh 196213 GB1-A11(2-52)-Strep ...Spinocerebellar ataxia 22 Bernard Weinstein 21238 PKC gamma CAT PRKCG HA CMV Spinocerebellar ataxia 21 Bernard Weinstein...TLS 1: hTLS.pBSKS(+) FUS T7 ALS David Ron 21828 TLS 2: hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C...ApoE Alzheimer's Sohail Tavazoie 51436 pGL3Basic-ME.2/ApoEpromoter APOE ApoE Alzheimer's Sohail Tavazoie...

  11. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons... the human brain through the development and application of innovative tools enabling large-scale real-time...or when BRAIN Initiative grants are noted in publications associated with Addgene materials. Plasmids ...Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore...108912-AAV9 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 Cation channelrhodopsin ChroME targeted to the neuronal...

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...hSyn promoter rtTA3 Wei Xu 26803 pEnt L1L3 EF1a-tTA-2 Gateway entry vector to express tTA from EF1α promoter...expression and improved dynamic range . Hum Gene Ther, 22 (2), 166–176. https://doi.org/10.1089/hum.2010.099 (Link... Return to Top Other Tet Applications Explore some other popular applications of tetracycline systems ...for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative Doxycycline...vectors for transactivators, and vectors for other applications, or search our collection for all dox-regulated... Tet-On 3G transactivator Tet-On 3G rtTA Elena Cattaneo 104543 PB-EF1a-TetOn3G PiggyBac vector expressing...-tag from TRE3G promoter for Tandem-Affinity Purification. Yannick Doyon 20321 TetO-FUW-OSKM Tet-On inducible...

  13. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...expressing Rep2 and Cap1 James M. Wilson 232199 pAAV2/2 AAV2 AAV packaging plasmid, expressing Rep2 and Cap2...plasmid (or Rep/Cap plasmid) containing the AAV replication (Rep) and capsid (Cap) genes necessary for the...the AAV genome, but are required for AAV viral replication. This collection provides a curated selection...production suitable for a variety of serotypes and applications. Read our AAV vector guide for more information...plasmids, or RepCap plasmids, encode the AAV replication and capsid proteins necessary for AAV vector ...expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S...expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1...

  14. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...AAV2 vectors in the mouse retina. Mol Ther . Feb;19(2):293-301. PMID: 21045809 Other citations include: ... any application Balanced Salt Solution (BSS) An ophthalmic solution suitable for any application TMN200...ordering information. ID Name Promoter Description Category PI 157970 pTR-UF11 chimeric CMV/Chicken Beta actin...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ...Golden Gate TALEN Kit and are used in place of pTAL1, 2, 3, or 4. For both plasmids sequence positions 1214...Golden Gate TALEN kit. The GoldyTALEN scaffold is truncated at both the N and C terminus and induces mutation...pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed...in vitro mRNA synthesis from the T7 promoter. Truncations were introduced to the N- and C-terminus of the...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...expression in mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller...

  16. Fluorescent Proteins: FRET

    Type
    Collection
    ...referred to as intermolecular or bimolecular FRET) or (2) conformational changes within a protein where the...pairs Michael Davidson Lab (Unpublished) FRET Indicators - Live and Fixed Cells Additional Resources at...

  17. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisomes Peroximal targeting signal (SKL) mCherry...Centrin2 Centrioles (dependent on cell cycle) Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles... Protein PI 71367 Human Beta-catenin GFP Adherens junctions beta-Catenin EGFP Alpha Yap 67937 mouse E-...Michael Davidson 55001 mCherry-Beta-Catenin-20 Adherens junctions beta-Catenin mCherry* Michael Davidson 30313...Fluorescent Protein Collection or search our full catalog for a marker for your structure of interest. Additional...

  18. Neurodegeneration Research Collection

    Type
    Collection
    ...different inherited genes: Presenilin 1, Presenilin 2, and APP. The majority (> 90%) of individuals develop...loci seem to be associated with the disease and implicate a wide range of cellular processes. The vast majority... consists mainly of managing symptoms through medication and surgery. PD primarily involves the malfunction...available from Addgene, sortable by type of genetic modification and target species. You can also find our guide...synaptic transmission with improved glutamate indicators . (Aggarwal et al. bioRxiv. 2025) Monitor dopaminergic...window) A news website and information resource dedicated to helping researchers accelerate discovery and...Foundation (Link opens in a new window) A foundation dedicated to finding a cure for Parkinson's disease through...

  19. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...- Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression... Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag - ...and emission maxima (Stokes shift), useful for applications like multicolor imaging with better spectral...

  20. Retrograde AAV viral preps

    Type
    Collection
    ...sensor Jonathan Ting 83899 pAAV-mDlx-GCaMP6f-Fishell-2 mDlx GCaMP6f expression under the control of the mDlx...the functional catagories listed below. Narrow down the items available within a category by using the ...experiments can be found on the material pages for viral catalog items. Browse Available Retrograde AAV See our ... Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden...offers you even more options. Select from a vast catalog of eligible AAV plasmids and we’ll make the viral...
Showing: 41 - 60 of 61 results