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We narrowed to 94 results for: expression vector

Showing: 41 - 60 of 94 results

  1. Zinc Finger Consortium Reagents

    Type
    Collection
    ...Assembly method Nuclease Expression Vectors - Cloning vectors for expressing zinc finger FokI nuclease...Find reagents for engineering and expressing zinc finger proteins deposited by Zinc Finger Consortium... Addgene various reagents for engineering and expressing zinc finger proteins for distribution to academic...

  2. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...j.bbrc.2013.08.013. Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...both the gRNA and Cas protein act as all-in-one vectors, but their function is often limited to a single...

  3. Open Enzyme Collection

    Type
    Collection
    ...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification...their own enzymes by cloning them into an expression vector of their choice and transforming into E. coli... and protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens...top Additional Resources Bacterial Protein Expression Vectors BioBricks Foundation Plasmids Drew Endy Lab...and codon-optimized the Open Enzymes parts for expression in Escherichia coli bacteria. Researchers can...purification ( pET28a-LIC or other pET vectors are recommended). The selection marker for all plasmids in ... are also compatible with the E. coli Protein Expression Toolkit (Link opens in a new window) — all Open...

  4. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...use double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The concept...start site to have high expression, so adding this G could help with expression, though it is possible ...you need to have four spacers co-expressed and using PX335 to express the Cas9n (nickase). Homologous ...concept of the double nickase system is that you can express two different chimeric gRNAs with the Cas9 nickase...oligo when cloning my target spacers into the PX330 vector backbone? There is no need to add the NGG PAM sequence...backbone that uses the human U6 promoter to drive expression, is it necessary to add a G nucleotide to the...possible for the plasmid to still express without the G. Because the G is only one base, the Zhang lab usually...

  5. CRISPR Plasmids - Plants

    Type
    Collection
    ...Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors such...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...CRISPR expression plasmids that have been designed for use in plants. CRISPR...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...cloning method. When using CRISPR, you will need to express both a Cas protein and a target-specific gRNA in...

  6. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...protein. Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine with N-terminal...Cyan Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene of interest...Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal...Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene...Mammalian Express a gene of interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene...Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal...Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover Green Mammalian Expresses Clover...

  7. Viral Production

    Type
    Collection
    ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Transducibility Some viral vectors are tested in vitro and in vivo for gene expression and/or function. These...Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles... Viral Vector Packaging Service Viral Production Viral Production...to determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet...

  8. Optogenetics Plasmid Collection

    Type
    Collection
    ...These proteins can be expressed in specific neurons, often utilizing viral expression systems, to activate...and kinetic properties as well as improve the expression and membrane targeting in host cells. Other microbial...fluorescent protein to the opsin in order to track its expression and localization. Many other types of proteins...optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication Return to top Optical...other terms. ID Plasmid Description Gene/Insert Vector Type Promoter PI Publication Return to top Do you...

  9. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Publication Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...below. Use the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA (e.g...Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ... editing plasmids. Plasmids are available for expression in mammalian systems, bacteria, plants, and Drosophila...

  10. Serotype Testing AAV

    Type
    Collection
    ... Viral Vector Packaging Service AAV Serotype Testing AAV Viral Vector Packaging Service: ...available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing: AAV1...addition to the standard 100 µL sample size, the vectors listed below are also available as 20 µL. The ...does not have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825... (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information about ...50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For information... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...

  11. Adenovirus Plasmids

    Type
    Collection
    ...Collection of adenovirus shuttle vectors and strains...Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are a class of medium-sized...kb), high titers, and high levels of transgene expression. Additionally, these viruses are able to target...Disadvantages of adenoviruses include transient transgene expression, as well as the substantial immune response induced...Block 6 with deletion in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral...Block 1 with deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production...For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for plasmid without...

  12. TALEN Engineering

    Type
    Collection
    ...TALEN Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors...sites and to simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly...

  13. Mammalian RNAi Tools

    Type
    Collection
    ...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...constitutive expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing.... shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral...Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with...

  14. Retrovirus Plasmids

    Type
    Collection
    ...Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope...GFP MoMLV U6-driven plasmid for shRNA expression; also expresses GFP. See pMKO.1 puro for Puro resistance... to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression; also expresses... 9044 pMIG MSCV Plasmid for transgene expression; also expresses GFP. Also see pMIG-w , a variant that...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for additional...pLncEXP CMV/MSV lncRNA expression plasmid Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in mammalian...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an...

  15. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...repressor peptide like KRAB, can knock down gene expression by interfering with transcription. Design your...transcriptional inhibition. Plasmids are available for expression in mammalian systems, bacteria, plants, and yeast...

  16. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the... plasmids for activating or upregulating gene expression in various model organisms. CRISPR...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...transcriptional activation. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...

  17. Plasmid Collections

    Type
    Collection
    ...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ... Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...

  18. Chemogenetics Plasmids

    Type
    Collection
    ...Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics vs. Optogenetics Chemogenetics...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Tags PI Do you have suggestions for...

  19. CRISPR Plasmids and Resources

    Type
    Collection
    ...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety... fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems...

  20. Lentivirus Plasmids

    Type
    Collection
    ...Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid...pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N...MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope... (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See article for more...constitutive transgene expression, contains intron that gives you high expression. Trono 12254 pWPI 2nd... plasmid for cDNA expression Ramalho-Santos 24129 pULTRA 3rd bi-cistronic expression of EGFP and the gene...pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dtomato...
Showing: 41 - 60 of 94 results