We narrowed to 80 results for: expression vector

...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...constitutive expression as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing.... shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral...Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with...

...TALEN Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors...sites and to simplify the construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly...

... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...Find bacterial expression plasmids that can be used for protein purification, protein visualization, ...

... top Empty Prime Editing gRNA Vectors A selection of empty gRNA vectors suitable for prime editing are...below. Use the search bar to find a gRNA vector based on expression system, promoter, the type of gRNA (e.g...Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI...

...mammalian expression systems. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page... CRISPR Mammalian Expression CRISPR Plasmids: Mammalian Expression Activate Base Edit Cut Epigenetics...

...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ... Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...

...Transl Med. 2013) Express human DNMT1 with a Myc tag. (Li et al. J Biol Chem. 2006) Express human ATM with...Science Collection . Express EGFP-tagged wt and mt tau . (Hoover et al. Neuron. 2010) Express EGFP-tagged wt...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. Study...Flag tag. (Bennett et al. Heliyon. 2020) Use VAP expression plasmids to study ER contact with other organelles...Convert iPSCs to neurons with inducible lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer...Ascl1 (Herdy et al. Elife. 2019) Use plasmids expressing SOX10, OLIG2, and NKX6.2 transcription factors...

...a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors... the specific cell type where the receptor is expressed, these receptors can be used to artificially activate...chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging PI Additional... Additional Resources Chemogenetics Guide Viral Vector Packaging Service In-Stock AAV: Chemogenetics Fluorescent...

...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety... fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor...epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems...

...cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...design and cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning...

...a site within that gene. To construct ZFN expression vectors, DNA encoding zinc finger arrays should be...sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc...plasmids also available through Addgene--the specific vector to use depends on the length of the spacer sequence...

...to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. TP53 Mutagenesis...Broad Target Accelerator Plasmids: Collection of vectors from the Boehm , Hahn , Meyerson , and Root Labs...pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing cDNAs ... Lab RAS Pathway Clone Collection 2.0 : Set of vectors from the Esposito Lab for use with the Gateway®...signaling studies, including mutant, knockdown, and overexpression constructs. Each collection page listed below...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Zebrafish"...Zebrafish". Cut ID Plasmid Gene/Insert Vector Type Promoter PI Publication Return to top CRISPR Resources Addgene...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Plant". Activate...CRISPR expression plasmids that have been designed for use in plants. CRISPR...

...Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...

...recombinant antibodies (rAb) encoded by mammalian expression vectors, human embryonic kidney cells are transfected...encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified from the supernatant...retained. If the target antigen is not endogenously-expressed (e.g., using an anti-GFP antibody), the target...target antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were...tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens...species-specific HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the...

...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Parasite"....CRISPR expression plasmids that have been designed for use in parasites. CRISPR...

...while also adding additional filters such as expression, vector type, species, and more. Plasmids Browse ...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...Libraries CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome...

...codon-optimized for expression in bacteria and cloned into an IPTG-inducible His-tag expression vector. All His-tags...sustainable MDH-related CURE focusing on protein expression, structure and function, kinetics and mechanisms...

...genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library containing...Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection You...