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TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... our guides to find the plasmids and science information you need before starting your experiment. Genome...
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Optogenetics Plasmid Collection
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
CRISPR Plasmids - gRNAs
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this....org for more information and assistance with the deposit process. Want more information on the wide variety...publication or plasmid information page, or contact the depositing PI for more information on the experimental...publication associated with each plasmid for more information on how it was originally used. Here are a few... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
DNA Service - Cloning Grade DNA
TypeCollection...let us know using the Cloning Grade DNA Suggestion Form . Please note that this does not guarantee that ... our collection. The plasmids available in this format include a variety of popular plasmids and backbones...individual plasmid page to find current pricing information Standard Quality Control Plasmid verified by ...Industry PI FAQ What Quality Control analyses are performed on my cloning grade DNA order? For each cloning...DNA preps of this plasmid available, but helps to inform us of which plasmids would be most helpful to requesting... -
Chemogenetics Plasmids
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
CRISPR Plasmids - C. elegans
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
Synthetic Biology - Overview
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...world to facilitate the sharing of parts and information. Addgene can help you to share your synthetic...Jaramillo Lab Riboregulation Keasling Lab BglBrick Platform , Terpenoid Production , and Propionate Induction... -
Serotype Testing AAV
TypeCollection...fill out our form to let us know what you would like to see available in viral prep format. Please note...economical choice that can be used to validate the performance of various serotypes before conducting larger...larger scale experiments. For more information, see our full AAV inventory or our AAV production page . Note...direct GFP expression from the CAG promoter. For information about each catalog item, including the titer ...expression from the human synpasin promoter. For information about each catalog item, including the titer ... -
Antibody Guide
TypeCollection...light chains. These four domains come together to form a “Y” shape, as shown in Figure 1. The two arms ...the five human isotypes. Some isotypes naturally form multi-antibody units, while others exist as monomers...may want to identify or collect the protein in any form it may be present in. However, polyclonals can vary...antibody, for each antibody used. Blank controls in the form of sample buffer must also be run. Unique samples...fluorophores. Horseradish peroxidase (HRP) HRP performs a chemiluminescent reaction in the presence of...activated and its output measured. This method can be performed using the indirect detection method via a conjugated... able to recognize the protein in its native conformation. Attachment of the antibody to the beads is ... -
Plan Your Experiment
TypeCollection...potential consequences of having a non-functional form of your protein expressed in your system. HDR : ... to produce the Cas enzyme, and the enzyme then forms a complex with the gRNA for CRISPR activity. Because...purified Cas protein and in vitro transcribed gRNA to form a Cas-gRNA complex. You then delivery the RNP to... and large insertions or deletions. For more information on the wide range of CRISPR technologies, visit...Selecting the genetic manipulation you plan to perform will help you narrow down which components you’...edit, ideally less than 10 bp away. For more information, read our three tips to improve HDR efficiency...your Cas protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and Spike Variants... -
Adenovirus Plasmids
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...particles. Read our Adenoviral Guide for more information about: Adenoviral components Adenoviral plasmid... -
CRISPR Plasmids - Drosophila
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
Recombinases AAV Preps
TypeCollection...fill out our form to let us know what you would like to see available in viral prep format. Please note...expression. See our Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name ... -
Retrovirus Plasmids
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...mutagenesis). Read our γ-Retrovirus Guide for more information about: γ-Retroviral packaging systems Frequently... -
CRISPR Plasmids - Yeast
TypeCollection...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... -
Distribution to Industry
TypeCollection... Recipient Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist...Agreements on our Technology Transfer Information page for more information. To learn more about Addgene's MTA...Pooled Libraries Antibodies Ordering and MTA Information Featured Collections NEW NanoLuc and HaloTag ...account. Please see our Help Center for more information on pricing, payment, and billing. Industry Material... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...expression of target protein as N-terminal fusion form with Sumo tag Trx (thioredoxin) Protein purification...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...delivery. Visit our viral vector page for more information. Below are some common delivery methods. Delivery...plasmid View our Adenovirus Plasmids page for more information Genome Modifications, Reporter Assays, mRNA Regulation... Cas9 into plants by Agrobacterium-mediated transformation Dual-sgRNA_hU6-mU6 - Lentiviral expression ...Tet-inducible lentiviral shRNA expression Find yeast transformation plasmids with G418 selection Find nematode ...ubiquitin promoter for Agrobacterium-mediated transformation Blasticidin Mammalian pLX304 - 3rd gen lentiviral...