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Showing: 41 - 60 of 133 results

  1. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones...purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification...identification of the transcription factor isoform in pooled screens. This collection and library can be...transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to ...

  2. Ras Pathway

    Type
    Collection
    ... gene it can be stuck in a constitutively active form, constantly activating pathways for cell growth ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... below. For proteins with multiple subunits or isoforms, individual links to each gene page are provided...gene. For components with multiple subunits or isoforms, individual links to each gene page are provided...E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP... SHC4 SHC (Src homology 2 domain containing) transforming protein SHOC2 SHOC2 leucine-rich repeat scaffold...Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of...

  5. Plasmid Collections

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... our guides to find the plasmids and science information you need before starting your experiment. Genome...

  9. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...let us know using the Cloning Grade DNA Suggestion Form . Please note that this does not guarantee that ... our collection. The plasmids available in this format include a variety of popular plasmids and backbones...individual plasmid page to find current pricing information Standard Quality Control Plasmid verified by ...Industry PI FAQ What Quality Control analyses are performed on my cloning grade DNA order? For each cloning...DNA preps of this plasmid available, but helps to inform us of which plasmids would be most helpful to requesting...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...or after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases of... of Alzheimer's disease. Of these early-onset forms, a fraction of these are due to a mutation in one ...NeuroMabSeq platform. (Mitchell et al. Sci Rep. 2023) See More Fluorescent Proteins Find information on Addgene's...has assembled the following to help you find information and plasmid collections that may help with your...human iPSC-derived neurons with a CRISPRi-based platform . (Tian et al. Neuron. 2019) See More AAV Viral...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. Convert...al. PLoS One. 2011) See More Antibodies Find information on Addgene's collection of plasmid-based antibodies...

  14. Synthetic Biology - Overview

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...world to facilitate the sharing of parts and information. Addgene can help you to share your synthetic...Jaramillo Lab Riboregulation Keasling Lab BglBrick Platform , Terpenoid Production , and Propionate Induction...

  15. Serotype Testing AAV

    Type
    Collection
    ...fill out our form to let us know what you would like to see available in viral prep format. Please note...economical choice that can be used to validate the performance of various serotypes before conducting larger...larger scale experiments. For more information, see our full AAV inventory or our AAV production page . Note...direct GFP expression from the CAG promoter. For information about each catalog item, including the titer ...expression from the human synpasin promoter. For information about each catalog item, including the titer ...

  16. Adenovirus Plasmids

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...production. Read our adenoviral vector guide for more information about adenoviral components, generations, viral...

  17. Antibody Guide

    Type
    Collection
    ...light chains. These four domains come together to form a “Y” shape, as shown in Figure 1. The two arms ...the five human isotypes. Some isotypes naturally form multi-antibody units, while others exist as monomers...may want to identify or collect the protein in any form it may be present in. However, polyclonals can vary...antibody, for each antibody used. Blank controls in the form of sample buffer must also be run. Unique samples...fluorophores. Horseradish peroxidase (HRP) HRP performs a chemiluminescent reaction in the presence of...activated and its output measured. This method can be performed using the indirect detection method via a conjugated... able to recognize the protein in its native conformation. Attachment of the antibody to the beads is ...

  18. Plan Your Experiment

    Type
    Collection
    ...potential consequences of having a non-functional form of your protein expressed in your system. HDR : ... to produce the Cas enzyme, and the enzyme then forms a complex with the gRNA for CRISPR activity. Because...purified Cas protein and in vitro transcribed gRNA to form a Cas-gRNA complex. You then delivery the RNP to... and large insertions or deletions. For more information on the wide range of CRISPR technologies, visit...Selecting the genetic manipulation you plan to perform will help you narrow down which components you’...edit, ideally less than 10 bp away. For more information, read our three tips to improve HDR efficiency...your Cas protein and gRNA, which you generally perform by chemical transfection or electroporation. Expression...

  19. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and Spike Variants...

  20. Retrovirus Plasmids

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...Read our Gamma-retroviral Vector Guide for more information about gamma-retroviral components, viral production...
Showing: 41 - 60 of 133 results