...targets adenenine, NG targets thymine, and NN targets guanine (though NN can also bind adenenine with lower...approximately every 35bp. Researchers are still determining the importance of context for each TAL effector...be used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments in an...to hone these tools and the researchers who are learning to wield them. Learn more about Addgene’s TAL ...

... when cloning in E. coli . This alteration enables the generation of a single vector containing Cre and...Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs Cre Reporters & Tools...Cre alternatives from Gerald Rubin's lab. Cre-containing Plasmids Scientists have developed ways to tightly...deleting the floxed portion of a construct during the cloning process). VCre and SCre are alternative Cre recombinases...pAAV.cTNT.iCre iCre and tdtomato Chicken cardiac troponin T AAV Pu 70120 pEMS2159 iCre with MCS for inserting...132551 AAV-U6-gRNA-TnT-Cre Cre Chicken cardiac troponin T AAV Pu 134308 pCAG-Synaptophysin-TdTomato-IRES2...the expression of one gene while simultaneously turning on another. The FLEx switch system takes advantage...

...assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and...plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA, based on the Zhang... also provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB...system depends on MMEJ (microhomology-mediated end-joining), an alternative DSB repair pathway, instead of...were created using CRISPR and the donor plasmids containing homology arms and EGFP are available at Addgene...

...available at Addgene. The collection can be used for screening transcription factors for cellular programming... The individual clones, or the pooled library containing the entire pooled collection, can be purchased.... This collection and library can be used for screening transcription factors for applications such as....026 . TF ORF barcode PCR for NGS QC and bulk screening (85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...

...kinase that anchors a signaling network for transitioning between anabolic and catabolic states. Ras Ras...from the Boehm , Hahn , Meyerson , and Root Labs containing wild-type and mutant alleles described in the... Set of vectors from the Sabatini & Wood Labs containing cDNAs for studying oncogenic signaling pathways...from the Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction... Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer...

...with a wide variety of Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...preferentially repaired in the cell by non-homologous end joining (NHEJ); a mechanism which frequently causes insertions... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express... same cell at the same time. Single plasmids containing both the gRNA and Cas protein act as all-in-one...

...with a wide variety of Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express... same cell at the same time. Single plasmids containing both the gRNA and Cas protein act as all-in-one...

...base editors-- cytosine base editors (CBEs) and adenine base editors (ABEs) --can be used to generate single...or a G to A on the opposite strand.) Likewise, adenine base editors have been engineered to convert adenosine...the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have...evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved...domain is fused to a Cas9 protein to create the adenine base editor. Both types of base editors are available...

...with a wide variety of Cas-containing plasmids. gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance...preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express... same cell at the same time. Single plasmids containing both the gRNA and Cas protein act as all-in-one...

...that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox...Tools for Use in Zebrafish Looking for plasmids containing your zebrafish gene of interest? Search Addgene's...biotin labeling in zebrafish. pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination... Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms...zebrafish as a model organism and to foster advanced training of young scientists in Latin America. The Zebrafish...

...antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into... two types of antigen specificity: monoclonal, meaning many copies of one clone that are specific to a... a certain epitope, or polyclonal, meaning there are many clones that are specific to multiple epitopes...using permeabilization, antigen retrieval, and/or sectioning. Block to reduce non-specific binding of antibodies...used currently are in the mid-forties. When designing a multiplex assay, selection of the fluorophores... your target epitope in the assay type you are running. Sufficient validation data specific to your antibody...Validation of an antibody is a process separate from running appropriate controls in your assay. There are five...

... Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene... growth and proliferation. mTOR, a serine/threonine kinase, is found in two complexes, mTORC1...LST8 homolog LKB1 Also known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin... Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini & Kris Wood) References...

...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from either ...with accurate, current, accessible information concerning the genetics, genomics and biology of C. elegans...

...unique potential to continuously divide while maintaining an undifferentiated state. Stem cells are classified...Sox2, Klf4, and c-Myc from four separate plasmids Defining molecular cornerstones during fibroblast to iPS...Neurons Lentiviral Human Small molecules enable neurogenin 2 to efficiently convert human fibroblasts into...human fibroblasts using self-regulating and nonintegrating viral vectors. Stem Cell Reports. 2014 Nov ...

...protein used in most studies to date. Neisseria meningitidis (NM): Significantly smaller than the above Cas9...protocol for synthesizing gRNAs: Cas9 orthologs gRNA cloning protocol 104.1 KB This set of plasmids is described..., human optimized 48670 M-NMcas Mammalian N. meningitidis Cas9 expression, human optimized 48673 M-NM-... human optimized 48646 DS-NMcas Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin...

....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748...shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono 11795... the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...

...Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls learning in the lateral amygdala... N. 2019. Excitation of diverse classes of cholecystokinin interneurons in the basal amygdala facilitates...McElligott ZA, Holmes A, Heisler LK, Kash TL. 2016. Serotonin engages an anxiety and fear-promoting circuit ...

...Gradinaru 206504 AAV-KP1 AAV-KP1 AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter...206512 AAV-LK03 AAV-LK03 AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter...Kay 206513 AAV-AM AAV-AM AAV packaging vector containing AAV2 rep and AM cap and partial AAV2 p5 promoter... 213974 AAV-DJ8R AAV-DJR AAV packaging vector containing AAV2-rep gene and CapDJR modified AAV-DJ8 capsid...

...list of EXPRESs plasmids used with AVEXIS for screening in zebrafish....collection of plasmids that was used for systematic screening of extracellular protein interactions to identify... references for more information: Large-scale screening for novel low-affinity extracellular protein interactions...

...Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection.... These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors...Pooled Libraries are useful tools for genetic screening experiments. These libraries target the solute...