We narrowed to 92 results for: nin

...Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-joining ligation fidelity and...Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic...exon 5–8. Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's...

...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...editing in C. elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified...spatial control of gene expression in C. elegans by combining expression of the FLP recombinase from either ...with accurate, current, accessible information concerning the genetics, genomics and biology of C. elegans...

...Gong L, He M, Zhou P, Paninski L, Li B. 2017. The central amygdala controls learning in the lateral amygdala... N. 2019. Excitation of diverse classes of cholecystokinin interneurons in the basal amygdala facilitates...McElligott ZA, Holmes A, Heisler LK, Kash TL. 2016. Serotonin engages an anxiety and fear-promoting circuit ...

.... These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors...pooled libraries are useful tools for genetic screening experiments. These libraries target the solute...NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning Content last reviewed: 12 August 2025...

...based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and the URA3 marker, the p415...p415 plasmid (Loqué et al., 2007) containing the TEF1 promoter and the LEU2 marker, or the pCEV-G2-Km ...Km plasmid (Vickers et al., 2013) containing the TEF1 or PGK1 promoters and the KanMX marker. ID Plasmid...

...Resources Protein-barcode Reconstruction by Iterative Staining with Molecular annotations (PRISM) is a set of...these protein barcodes allow for efficient machine-learning segmentation of neurons and proofreading of neuron...microscopy and reconstruction of neurons with machine learning. Adapted from Park & Sheridan et al. (2025). PRISM...

...suffix sequences are given for the coding strand , meaning that the suffix will need to be reverse complimented...should be avoided whenever possible. Other popular cloning strategies for the assembly of synthetic parts ...systems such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common...

...concentration is confirmed by Coomassie staining. For Coomassie staining, the sample is separated via a denaturing...formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible...

...multi-plasmid systems. These systems consist of a plasmid containing the genetic information to be delivered (transfer... or shuttle plasmid) and one or more plasmids containing the necessary components of the viral genome ...Many viral plasmids are also self-inactivating, meaning that they can only complete one round of transcription...

...Histones 109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin Adherens junctions 107579 RAB5A-mEGFP AICSDP-39...stress granules 114411 TNNI1-mEGFP AICSDP-34 mEGFP Troponin I, slow skeletal type Sarcomeric thin filaments...Factor 124607 ACTN2-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP...

...activating factor 1 ATM ATM serine/threonine kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed...PIRH-2 Ring finger and CHY zinc finger domain containing 1 (RCHY1); E3 ubiquitin protein ligase PTEN Phosphatase...

...suited to simplify the cloning process as it can be used in blue–white screening . Adenoviral Vector ID...promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express...

...plasmids: A transfer plasmid (or cis plasmid) containing the transgene or genetic elements of interest...cells. A packaging plasmid (or Rep/Cap plasmid) containing the AAV replication (Rep) and capsid (Cap) genes...determine the AAV vector serotype. A helper plasmid containing adenoviral or herpesviral helper genes. These...

...collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice ...Jennifer Molloy Lab Plasmids Blog: Golden Gate Cloning...

....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy cloning. David ... the gene of interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...

...plasmid and is not compatible with other plasmids containing high copy ColE1/PUC-like origins. Therefore, ...at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions...domain of NEDD4-2 (Pooled Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also...

...mediate the integration of a large DNA payload containing an attB target site into a genomic attP site....the expression of one gene while simultaneously turning on another. The FLEx switch system takes advantage... target location for integration of a plasmid containing a gene of interest and another FRT site. Flp ...

...fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is the presence of three...resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute to this community...

...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...collection for community. If you have created plasmids containing MAPK and would like to add to this collection...

... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology...libraries. Pooled Library Types Barcoding CRISPR Screening Surface Display cDNA Depositor Collections A modular...