We narrowed to 63 results for: organization Addgene

...gene expression Lentiviral backbones made by Addgene Addgene has an extensive collection of plasmids for...A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function...to consider. Here is a guide to a selection of Addgene's empty vector backbones. For the most part, we ...promoter that will be functional in your host organism. Host Relevant Promoters Representative Empty ..., or zebrafish expression GST plasmids made by Addgene MBP Protein purification pET28-MBP-TEV - Bacterial...efficiency, but do NOT integrate into the host genome Addgene has an extensive collection of plasmids for packaging... shRNA expression Retroviral backbones made by Addgene Adenoviral High transduction efficiency, but do...

... proteins and cells, but imaging in deep-tissue organs or whole animals presents unique challenges. Genetically-encoded...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and...this service. Select any eligible plasmid in the Addgene repository in your choice of seven serotypes and...more about AAV serotypes and tissue tropism on Addgene's blog . All-in-One Pricing The price for our Packaged...production Density centrifugation purification Addgene’s comprehensive quality control process: Titering...Approval 1+ business days, varies by requesting organization Viral vector preps remain subject to the terms...remember to share your protocols and results through Addgene’s Data Hub . By contributing your AAV vector experience...let you know whether the item can be packaged. Addgene does not perform functional or in vivo testing....

.... Additional Addgene Genome Engineering Resources Learn more about CRISPR with Addgene's CRISPR Guide ...Escherichia coli is the most widely used bacterial organism due to its ease of use, speed of growth, and well...are also being used for their distinct features. Addgene distributes many plasmids that can help researchers...MalE, OmpA ID Plasmid Promoter Tags PI Additional Addgene Protein Purification Resources The Structural Genomics...localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous...and Pichia pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and...Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli Strains for Protein Expression Protein...

...generate these lines have been deposited here with Addgene for distribution to the research community. With... Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human... with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure.../Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell. 28 (21), 2854–2874. https://...

...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...BFP Ervin Welker Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service....researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...appropriate left and right homology arms. See Addgene's HDR blog post for more details. In some cases,...plasmid in question (see CRISPR protocols from Addgene depositors ). Browse empty gRNA expression vectors...you may need to look into other options. Browse Addgene's collection of CRISPR plasmids . Delivery Using...conducting genome-wide screens using CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV ...

...plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids.... TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc ...amino acid repeats in the TAL effectors. View Addgene's TALEN Guide. These collections and accompanying...Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector...Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... Neurodegeneration Research Collection Addgene has assembled the following to help you find information... some of the relevant collections available at Addgene. Amyotrophic Lateral Sclerosis Amyotrophic lateral...Tools Find CRISPR pooled libraries available from Addgene, sortable by type of genetic modification and ...2011) See More Antibodies Find information on Addgene's collection of plasmid-based antibodies, including...More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...CHDI Foundation (Link opens in a new window) An organization with a mission to rapidly develop therapeutics...expression plasmids to study ER contact with other organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically...

...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana ...As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids . To review performance...data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository...produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and specific ... produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter...

...Reading and Resources Addgene Blog: Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your ... to help you plan your experiment. Tables are organized by color of the fluorescent protein. The content...covalently bind a synthetic substrate conjugated to an organic fluorophore or other small molecule. By using different... Expression Return to top Additional Resources Addgene Collections Empty Backbones Michael Davidson Fluorescent...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Explore Addgene's collection of luciferase plasmids for gene expression assays and bioluminescent reporters...responsible for bioluminescence found in a variety of organisms, ranging from bacteria to aquatic animals to insects...The most popular types of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...expert to see results! Getting Started with CRISPR Addgene has empowered researchers to further develop the...applications. For more information on CRISPR, check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR... CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models: Mammalian systems...

...Browse Addgene's collection of plasmids for the generation and study of stem cells, from iPSC generation...and have the capacity to give rise to an entire organism. iPSC technology removes the possible ethical ...may be difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs...know which method you would like to use, browse Addgene's collection of Reprogramming Plasmids in the table...cells or fully differentiated cell types. Browse Addgene's collection of Differentiation Plasmids below. ...inherent disadvantage of iPS cell technology. Browse Addgene's collection of Transdifferentiation Plasmids below...below will help you find plasmids available from Addgene that can be used to create iPSCs or another cell...

...structures or organelles, to observe cytoskeleton remodeling or blocked membrane traffic. Addgene has assembled... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ...Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Content last reviewed...localization patterns for colocalization studies or organelle labeling. Plasmid... fusion proteins that are targeted to various organelles or subcellular structures. To compare with a ...Structures Eukaryotic cells contain a variety of organelles and subcellular structures, each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...

...window) . Addgene Website & Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101...cell lines Viral service at Addgene Viral plasmids and resources at Addgene Blog CRISPR topic page How ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...You Use a CRISPR Library? CRISPR libraries from Addgene are available in two formats: as liquid DNA, or...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...located. The majority of the CRISPR plasmids in Addgene’s collection are from S. pyogenes unless otherwise...

...orthogonal pairs of aaRS and tRNA that work for one organism may not work for another. The orthogonal aaRS ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...specifically localize to cellular compartments or organelles. Addgene’s database includes many fluorescent biosensors...biosensors can be introduced into cells, tissues, or organisms for long-term imaging and can be designed to specifically...sensors for various target RNAs Additional Resources Addgene Blog: Biosensors Optogenetics Plasmids Viral Service...Scaffold for inorganic phosphate biosensor Development of a Reagentless Biosensor for Inorganic Phosphate...vs. in vivo, cytoplasmic vs. extracellular vs. organelle-localized). Browse, sort, or search the tables... Vivo Imaging of Ca 2+ Dynamics in High-Ca 2+ Organelles. Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(...sensors (green, red, or blue/green) Imaging intraorganellar Ca 2+ at subcellular resolution using CEPIA...

...SynBio Genome Editing Gene Silencing Reporters Addgene’s repository contains many plasmid tools that were...other species. In addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections...engineering and gene expression to reporter systems. Addgene’s collection includes plasmids containing promoters...plasmids for gene editing in plants. Please refer to Addgene's Plant CRISPR Resources for a full selection of...truncatula . Return to top Other Plant Resources Addgene Blog Blog Posts on Plant Biology RUBY-Red Siliques...arbuscular mycorrhiza-inducible promoters to label organelles, membranes and cytoskeletal elements in M. truncatula...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...