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...infection. Dapeng Zhou Other Resources Protocols Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral...Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses (DOCX, 184...plasmids for viral pseudotyping with SARS-CoV-2 spike protein. Collections...virus particles with the SARS-CoV-2 spike (S) glycoprotein on the envelope surface. Pseudotyped virus particles...have been pseudotyped with the SARS-CoV-2 Spike protein and you will find examples of each in Addgene's...Variants Full-length wildtype SARS-CoV-2 spike protein is inefficient for pseudotyping; several modifications...the search bar to find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant....

...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols 2013). SpCas9 Cas9..., Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct ...Website You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9...co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9...Synergistic Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous... stemloop 2 The MS2-P65-HSF1 activation helper protein Full references are below. Pooled genome-wide human...

...Voytas DF, Joung JK. Nature Protocols . 2009;4(10):1471-501. doi: 10.1038/nprot.2009.98. Epub 2009 Sep 17...Resources A detailed protocol for practicing OPEN is available in the 2009 Joung Nature Protocols paper . How ... Scot Wolfe Lab plasmids Detailed Information Protocols & Resources Contents of Kit Original Publication...Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder...

...Collection How to Use Plasmids Additional Resources Protein-barcode Reconstruction by Iterative Staining with...combinations of cell-filling antigenically distinct protein tags (or "bits"), creating over 100,000 unique ...combinations detectable by antibodies. All of the PRISM protein tags have been validated for use in expansion microscopy...super-resolution light microscopy. In brain tissue, these protein barcodes allow for efficient machine-learning segmentation...al. (2025) use plasmids expressing the barcode proteins under the constitutive CAG promoter (Addgene #242764...include using individual or subsets of barcode proteins to label and detect multiple injection sites in...representation. To read out the barcodes, each protein tag can be imaged with a corresponding primary ...

...of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution Protein Characterization...CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling from New England... Structural Genomics Consortium Solute Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging... constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for detecting ...Bahl A validation set of 96 unique, single-domain proteins that are structurally characterized (PDB-deposited...intellectual property rights, and provides liability protection for material providers. Addgene's deposit agreements...

...Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy, ALS...Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy, ALS...type, mutations, and/or tags such as fluorescent protein (FP) tags. Information on genes and associated ... hnRNPA2_LC_WT HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98658 hnRNPA2_LC_D290V HNRNPA2B1...HNRNPA2B1 His T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98659 hnRNPA2_LC_D220V HNRNPA2B1 His T7 ...T7 Multisystem proteinopathy, ALS Nicolas Fawzi 98660 hnRNPA2_LC_D230V HNRNPA2B1 His T7 Multisystem proteinopathy...

...enzymes for cutting protein sequences to remove unwanted parts, and DNA-binding proteins that can be fused...ID Plasmid Gene/insert 165509 pOpen-TEV protease TEV protease 165565 pOpen-RNAse A RNAse A 165575 pOpen-RNAse...-binding protein 165549 pOpen-ETSSB Extreme Thermostable Single-Stranded DNA-Binding Protein 165579 pOpen-dromedNK...ampicillin. Find more information on the enzymes and protocols for expression in the Open Bioeconomy Lab's crowd-sourced...Open Enzymes are also compatible with the E. coli Protein Expression Toolkit (Link opens in a new window)...

...constructs and routine protein and molecular biology techniques. Using a single protein with many available...Community (MCC) (Link opens in a new window) develops protein-centric Course-based Undergraduate Research Experiences...begin a sustainable MDH-related CURE focusing on protein expression, structure and function, kinetics and...post-translational modifications, and metabolic pathways and protein interactions. Plasmids A diverse set of wild-type...detailed clone information, please see the MDH Protein Clone Information Sheet, available to download ...explore MDH projects, find teaching resources and protocols, learn about regional hubs, access a teaching ...

...CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal...CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny neurons...CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons ...CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong cortical...CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic neurons... CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons... CN2237) Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons...

...for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids...somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells over time... lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic loci in...reporter system that allows direct assessment of lipoprotein abundance, size, and localization in high-throughput...studying neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab. A useful...useful “starter kit” for screening fluorescent protein fusion expression in organisms or cells in which ... zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene Blog Posts...

...Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors... libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more. Addgene...Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids...Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic...RESOLUTE Plasmid Collection for Solute Carrier Proteins (SLCs) Structural Genomics Consortium Zinc Finger...

...operon from E. coli : the tetracycline repressor protein (TetR) and operator ( tet O, a 19 nucleotide sequence...TCCCTATCAGTGATAGAGA). In normal conditions, the TetR protein binds to tet O, blocking transcription of the downstream... RNA promoter. And, most importantly, the TetR protein has been modified to provide two classes of tet-responsive...activation domain of the herpes simplex virus VP16 protein (Gossen and Bujard, 1992). This work also introduced...expression. Improved expression of the transactivator proteins in mammalian cells or other species by codon optimization... needed. Or, when inducing a potentially toxic protein you may need extremely minimal background expression... Julien Béthune 154939 dCas13d-dsRBD-APEX2 RNA-protein interaction mapping via Cas13-based APEX targeting...

...open-access articles, protocols, and other COVID-19 resource collections. Protocol: RNA Extraction Without... Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems. Depositor...against COVID-19 all rely on DNA encoding viral proteins as a first step for many different kinds of important...enable researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene partnered...

...SCG), which are primarily used for determining protein 3D structure and crystallography.... determine the three dimensional structures of proteins of medical relevance, and place them in the public...pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and TEV cleavage...pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and TEV cleavage...Toronto: www.thesgc.org/scientists/groups/toronto Protein Database (PDB): www.pdb.org Request Plasmids To...

...be fused to protein-interacting RNA aptamers, which recruit specific RNA-binding proteins (RBPs) tagged...tagged with fluorescent proteins to visualize targeted genomic loci. CRISPR imaging has numerous advantages...requires recruitment of many copies of labeled proteins to the given region. For example, chromosome-specific...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

... Antibodies targeting proteins for neuroscience research. Institute for Protein Innovation Collection ...antibody, we provide important details including the protein and plasmid sequence, the antigen sequence, and...Collection : Antibodies to highly conserved proteins and other difficult-to-target antigens that are intractable...Recipient Instructions Addgene Help Center Antibody Protocols...

... used chemogenetic receptors and are G-coupled protein receptors that have been engineered to respond ...muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid...κ-opoid receptor and couples to the Gi G-protein. hM3D(Gq) and rM3D(Gs) are activating, while hM4D(Gi) and...Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing...Chemogenetics Fluorescent Biosensors Fluorescent Proteins Guide Optogenetics Plasmids Optogenetics Guide...

...open-source' protocol for making customized zinc-finger arrays. Maeder ML et al. Nat Protoc. . 2009. Sept... OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc apoeb (apolipoprotein Eb) OZ529 and OZ530 OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg...aAGCTTCCTCttcattgGCTGACTGCa ldr (low density lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc...customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(12):1855-1867. PubMed PMID 20010934...

... AAV encode tools that can be used for cell or protein labeling. ID Name Expression System Activity Serotype...expression of human 2N4R microtubule-associated protein tau with P301L mutation. 1 Tsuneya Ikezu 203840... constitutive Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons...Cre-dependent Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons...Beta-Secretase to be expressed (1:1) with either ATLAS protein. 8 Don Arnold Don’t See What You’re Looking For...

...vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully). This next...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636... at the final assembly step in the Golden Gate protocol. Both plasmids 47388 and 47389 include a Flag ...deletion from the N terminus of the wild-type TALE protein (previously shown to preserve the DNA-binding ability...length human LSD1 at the carboxy-terminus of the protein. 49043 EMM67 49044 EMM71T Golden Gate TALEN 2.0...