We narrowed to 114 results for: Cre
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TypeCollection...next generation architecture has been shown to increase mutation induction when using TALENs. The FokI...Gersbach These new destination vectors can be used to create TALE-transcription factors and are used at the ...TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence...these plasmids along with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation...
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CRISPR Plasmids - RNA Editing
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort... -
CRISPR Plasmids - Activate Gene Expression
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the... -
CRISPR Plasmids - C. elegans
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your ... -
mTOR Pathway
TypeCollection... Tackling Cancers’ Drug Resistance with a New Screening Kit Background mTOR Pathway Plasmids mTOR Gene...mTORC1 promotes protein and lipid synthesis, and increases energy production through glycolysis to fuel these... Tackling Cancers’ Drug Resistance with a New Screening Kit (from David Sabatini & Kris Wood) References... -
Plant Plasmids and Resources
TypeCollection...Cloning The following collections are designed for creating plant expression vectors: A modular plasmid assembly... - Martin Parniske. GreenGate Cloning System to create plant expression vectors containing several cassettes... to understand how scientific publications are created. Papers from the plant biology literature are discussed... -
COVID-19 Resources
TypeCollection...is primed by the serine protease TMPRSS2. Image credit: Maya Kostman for the Innovative Genomics Institute...metalloprotease that cleaves hACE2, potentially increasing viral infectivity.( 6 ) PCP4 - (Purkinje cell... Affected by the Pcp4 Dose-Dependent [Ca 2+] i Increase in Down Syndrome Mice, Ts1Rhr . Do you have suggestions... -
Michael J Fox Foundation (MJFF) Plasmid Collection
TypeCollection...expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...topic. Michael J. Fox Foundation Plasmids MJFF has created a number of plasmids for expression of variants... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... -
KLF Research Plasmids
TypeCollection...plasmids and learn more about the scientists who have created and shared these plasmids....of this shared resource on Addgene. If you have created plasmids containing KLF, we encourage you to contribute... -
CRISPR Plasmids - Epigenetics
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort... -
CRISPR Plasmids - RNA Targeting
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...activity, experimental considerations, and more, created and maintained by the Doudna Lab . Browse, sort... -
CRISPR Plasmids - Zebrafish
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific... -
CRISPR Plasmids - Parasites
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs...Figure 1: Overview of the basic CAST mechanism. Created with BioRender.com. Browse, sort, or search the... -
Structural Genomics Consortium Plasmids
TypeCollection...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and... -
Zinc Finger Consortium: OPEN Reagents
TypeCollection...paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge the Principal...Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods... -
CRISPR Plasmids and Resources
TypeCollection...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...