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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 102 results for: Cre

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Showing: 61 - 80 of 102 results

  1. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...breaks in DNA instead of the double-strand breaks created by wild-type Cas enzymes. To use a nickase mutant...your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using...

  2. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and...

  3. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...CRISPR plasmids that create double strand breaks in DNA.... in the DNA. Indels often lead to frameshifts, creating loss of function alleles. To introduce specific...

  4. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target alterations and... Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse, sort, or search the...

  5. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with yeast codon-optimized...window) PMID: 24161108 (Link opens in a new window) Credits Contributing Authors Written by Dennis Botman in...

  6. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge the Principal...Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods...

  7. CRISPR Plasmids and Resources

    Type
    Collection
    ...Other Applications Screen CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused...

  8. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...each (these can be built into PCR primers when creating a new part, with an additional 5' clamping sequence...Features: Modified RFC 10 w/ start and stop codons; Creates in-frame fusions w/ linker Thr-Gly; Use N-parts...

  9. Viral Vector Guides and Plasmids

    Type
    Collection
    ...generate viral vectors for research and gene therapy. Created with BioRender.com. Wild-type viruses have one ...vehicles to deliver genetic cargo into cells. To create safe and effective viral vectors, wild-type viral...

  10. Microbiology Resources

    Type
    Collection
    ...used to reconstitute self-replicating microbes or recreate the diseases they cause. The standard format for...system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia...

  12. Resolute Plasmid Collection

    Type
    Collection
    ...pooled libraries are useful tools for genetic screening experiments. These libraries target the solute...window) PMID: 40355755 (Link opens in a new window) Credits Contributing Authors Written by researchers in ...

  13. Lentiviral Prep Service

    Type
    Collection
    ...Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes involved in specific...

  14. Fluorescent Proteins: FRET

    Type
    Collection
    ...imaging microscopy (FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor...by Eric Greenwald and the Jin Zhang Lab at UCSD Credits Contributing Authors Written and reviewed by the...

  15. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...at the central position. Introducing Hi-P for screening phosphorylation-dependent protein-protein interactions...domain of NEDD4-2 (Pooled Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also...

  16. Rett Syndrome

    Type
    Collection
    ...available directly from the labs in which they were created. Find their contact information by following the...

  17. TREAT-AD Plasmid Collection

    Type
    Collection
    ...validated resources for emerging targets in AD and creating high-quality, well-characterized lead molecules...

  20. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine...
Showing: 61 - 80 of 102 results