...are based on the pTT3 plasmid backbone which is described by Durocher et al. Nucleic Acids Research 2002...showing how AVEXIS works with a detailed protocol description: Avidity based Extracellular Interaction Screening...

...Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering of biological systems...Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate...

...envelope and packaging plasmids. ID Plasmid Type Description PI 8449 pUMVC Packaging Contains MoMLV gag and...plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo largeTcDNA MoMLV Expression...

...create and express plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy...for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre....

...lentivirus technologies. ID Plasmid Generation Type Description PI 12253 pRSV-Rev 3rd Packaging Contains Rev;...table and search "lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty...

...elegans expression Other Fluorescent Proteins Localization See full Fluorescent Protein Guide...

...plasmids and protocols needed for using them, are described below. Read our CRISPR 101 post for more information... the first publicly available collection of fluorescently tagged, human, induced pluripotent stem cell...

...lab determines a minimally functional MECP2 that rescues neurological defects of Rett syndrome. (Link opens...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...

...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong, et. al, 2013 Science ...co-transfect in HR donor. Alternatively, a new system described recently is the double nickase system, with PX335...

... nuclear receptor subfamily 2, group E, member 3 ESCS, MGC49976, PNR, RNR, RP37, rd7 NR2F1 nuclear receptor...Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories...immune response. Over 60 interleukins have been described. NK Cell Signaling Natural killer (NK) cells can...immune response. Over 60 interleukins have been described. Symbol Name Synonyms EBI3 Epstein-Barr Virus ...and standardized building blocks: 14 different fluorescent proteins, 10 mammalian promoters and enhancers...Plasmids 101: Monitoring Cell Mobility Using Fluorescent Proteins The Challenges of Cell Culture External...

...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations... enhancers for specificity and brightness of fluorescent labeling across brain regions and populations...

...the particular conditions of the experiment as described in the associated article (listed below by PubMed...within promoters. When possible, the categories described on Addgene's CRISPR Plasmids and Resources page...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...