We narrowed to 155 results for: Gene
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TypeCollection...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
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iPSC Neurodegenerative Disease Initiative Plasmid Collection
TypeCollection...used to create cell lines with endogenously-tagged gene variants or to differentiate human iPSCs into other...Find plasmids generated by the iPSC Neurodegenerative Disease Initiative (iNDI) here. Depositor...Stem Cell Research Neurodegeneration Research Collection The iPSC Neurodegenerative Disease Initiative...plasmid collection at Addgene. Figure 1: Schematic of iNDI iPSC line generation, phenotyping, and distribution...Collections iNDI iPSC Neurodegenerative Disease Initiative (iNDI) Collection You May Also Like... CRISPR ...Initiative (iNDI) project aims to generate and characterize single nucleotide variant lines in an isogenic cellular...NIH. Reference Ramos et al. 2021. Tackling neurodegenerative diseases with genomic engineering: A new stem... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a large selection...large genomic deletions. Compared to deletions generated by Cas9, large deletions created using Cas3 have...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
TALEN Engineering
TypeCollection...Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids...transcriptional activator target sites and then generates individual user-friendly graphical roadmaps for... -
CRISPR Plasmids and Resources
TypeCollection...transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression...Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a...factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR...CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection... Endogenous Gene Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human...Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens in a new window..., S. M., & Gunawardane, R. N. (2017). Systematic gene tagging using CRISPR/Cas9 in human stem cells to... The plasmids used to generate these lines have been deposited here with Addgene for distribution to the...Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular ...With these plasmids researchers will be able to generate fluorescently tagged proteins of key cellular ... with endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure... -
Botman-Teusink Yeast FP Collection
TypeCollection...marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene Kits MoClo-YTK ...constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization ...Bydder, S. F., Zhou, Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection...Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials Kurt Thorn Lab... -
Synthetic Biology - Sensing and Signaling
TypeCollection...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter Tags PI Publication ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
Synthetic Biology - Strains
TypeCollection...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...available from this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
Malate Dehydrogenase CUREs Community Collection
TypeCollection...a diverse set of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based...interactions. Plasmids A diverse set of wild-type MDH genes from several species and mutant clones from watermelon..., or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs ... -
Synthetic Biology - Overview
TypeCollection...contains pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and... pathways and auxiliary components. Networks and Gene Regulation Browse plasmids containing both naturally...Lab RNA Feedback Lu Lab Multiplexed/Programmable Gene Networks Mascher Lab Bacillus BioBrick Box Mukhopadhyay...about synthetic biology resources available at Addgene. Browse featured collections or depositing labs...engineering of biological systems for novel functions. Addgene is working with leading synthetic biologists around...facilitate the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids...signaling and environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including... -
Synthetic Biology - Metabolism
TypeCollection...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
TREAT-AD Plasmid Collection
TypeCollection...this table based on plasmid name, gene/insert, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...of drug targets in Alzheimer’s disease (AD) by generating validated resources for emerging targets in AD...IUSM-Purdue TREAT-AD centers will be deposited at Addgene and available to request. Plasmids Browse the table... -
Zinc Finger Consortium Reagents
TypeCollection...Arrays for Zebrafish Genes - Browse pre-constructed zinc fingers targeting specific genes Oligomerized Pool...Keith Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing... -
Synthetic Biology - Cloning and Genomic Tools
TypeCollection...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back to Top Shuttle...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
Retrovirus Plasmids
TypeCollection...your gene fused to eGFP expression Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...transfer plasmids. Want a retroviral plasmid with your gene of interest? Search our database for retroviral ...Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen for ...'s genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets integrated...to ∼8 kb), and infection results in long-term transgene expression. Some disadvantages of γ-retroviruses... -
MAPK Plasmids
TypeCollection...deposit@addgene.org to be paired with an Addgene scientist. MAPK Plasmids (organized by gene) Gene Alternate... numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
CRISPR References and Information
TypeCollection...identify positively or negatively selected sgRNAs, genes, or pathways. For use with genome-scale CRISPR-Cas9...CRISPR screens targeting a long or short list of genes provided by the user. Developed by the Fredrik Wermeling...sgRNA design tool for genome editing, as well as gene regulation (repression and activation). Genome support...target sites by off-target quality. Validated for gene inactivation, NHEJ, and HDR. Includes a wide range... to identify novel gRNA target sites in a single gene, as well as a target site common to a set of similar... Information Addgene Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection...transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors...10X scRNA-seq (PDF, 59.2 KB) Plasmids ID Plasmid Gene/Insert Pooled Library The MORF pooled library is... transcription factor collection, available at Addgene. The collection can be used for screening transcription... entire pooled collection, can be purchased at Addgene. Each transcription factor isoform has a unique...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
Promega Plasmid Collection
TypeCollection...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...contains luciferase reporter vectors for sensitive gene expression analysis and protein fusion vectors for...tracking protein degradation, analyzing CRISPR-mediated gene editing outcomes, and conducting high-throughput...Promega — now available through Addgene. As a purpose-driven nonprofit, Addgene offers expert support, educational...Browse Addgene's Luciferase Plasmid Collection Explore other Promega plasmids available at Addgene Find ...subunits come together to form an active enzyme and generate a bright luminescent signal in the presence of...window) blogs for technical tips and tools for genetic reporter assays (Link opens in a new window) and...