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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 120 results for: LAT

Showing: 61 - 80 of 120 results

  1. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...the spread of infection throughout the entire population. Type VI enzymes that function in mammalian cells...mammalian systems, Cas13a does not exhibit the collateral RNA degradation seen in bacteria. Want more information...

  2. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...changes, researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  3. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...

  4. CRISPR Plasmids and Resources

    Type
    Collection
    ...genome engineering. CRISPR Topic Page : Want the latest news on CRISPR? Experts cover CRISPR topics on ...insert large fragments of DNA. Transcriptional Regulation Activate dCas9 fused to an activator peptide ...Purify dCas9 fused to an epitope tag can be used to isolate a given genomic locus. Visualize CRISPR fluorescence...

  5. Retrovirus Plasmids

    Type
    Collection
    ...called γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and infection... variant that has WRE, a post-transcriptional regulatory element that increases export to the cytoplasm...retroviral plasmid Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber...

  6. Antibody Production

    Type
    Collection
    ...using Protein A or Protein G affinity columns. Formulation Buffer After affinity chromatography, antibodies...exchange using centrifugal columns. The final formulation buffer is phosphate-buffered saline containing...generated. The final antibody concentration is calculated from the standard curve. Using ImageJ software...

  7. Plant Plasmids and Resources

    Type
    Collection
    ...technologies: from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (..., computational and experimental resources that relate to the control of gene expression in the grasses...

  8. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...plasmid ( Addgene #157970 ). Viral preparations are formulated in one of the following three buffers. All buffers...serotype displays highly efficient transduction and lateral spread in the retina following subretinal injection...E531D) serotype has increased transduction and lateral spread in the retina following subretinal injection...

  9. CRISPR References and Information

    Type
    Collection
    ...and correspond to edited and non-edited cell populations. For more information, see the Hsiau et al. (.... protoSpaceJAM protoSpaceJAM is an all-around platform for CRISPR knock-in design at scale. It uses an...design tool for genome editing, as well as gene regulation (repression and activation). Genome support for...

  10. Synthetic Biology - Strains

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial Strains Search the table...

  11. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to cell-to-cell communications and environmental sensing...

  12. Synthetic Biology - Metabolism

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to metabolic pathways and components. Examples Include...

  13. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's ...Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library construction...

  14. KLF Research Plasmids

    Type
    Collection
    ...Keisuke Kaji Jonathan Katz Nathan Lawson Kathrin Plath Miguel Ramalho-Santos Derrick Rossi Michael Ruppert...family of at least 17 DNA binding transcriptional regulators with diverse roles in processes such as cell ...

  15. Depositor Collections

    Type
    Collection
    ...Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase CUREs Community (MCC) Collection ...Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection Synthetic Biology Voigt...

  16. MAPK Plasmids

    Type
    Collection
    ... signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...

  17. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed...

  18. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ... the CAPTURE system. The locus is subsequently isolated by affinity purification against the epitope tag...

  19. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding the ...

  20. Fluorescent Protein Guide

    Type
    Collection
    ...and whole mammals. Regulate Biological Activity Use fluorescent proteins to modulate biological activity...
Showing: 61 - 80 of 120 results