...repository contains many plasmid tools that were specifically designed for use in plants. These resources ...Multipurpose Toolkit enables targeted, specific modification of monocot and dicot genomes using genome engineering...MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes...an Old Favorite External Resources Arabidopsis Biological Resource Center (ABRC) (Link opens in a new window...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature...

...K-RAS, N-RAS, RASH1 ICAM1 intercellular adhesion molecule 1 BB2, CD54, P3.58 ICAM2 intercellular adhesion...repurposing of open access immunological assay data for translational and clinical research. Sci Data. Feb...and adaptive immune signaling and cell-cell communication. Plasmid...specific to any particular pathogen. It includes physical barriers such as the skin and mucosa, antimicrobial...molecules that enable cells of the immune system to communicate with each other across long distances. During...with virus and, like other cytokines, enable communication between cells of the immune system. Interleukins...are secreted by immune cells and coordinate communication between immune cells over the course of an immune...

...excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...fragments (which have become exons) determines the logical expression requirements of Cre and Flp (B,E). When...Robertson K, Osten P, Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular...

...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Selectable Marker PI Publication Last reviewed...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...bidirectional insertions and may not be suitable for applications where orientation matters. Type V-K systems ...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis...leishmaniasis), and others have been the focus of biomedical research for decades. Recent developments with...NHEJ. Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled Library...

...pX458 plasmid family. They have made several modifications to the the pX458 plasmid to expand its usefulness...developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh promoter with EF1alpha...Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L., Goodson, N. B., Park, K. U.,... alters photoreceptor and bipolar cell fate specification, yet differs from deleting both genes . Development...

...SARS-CoV-2 infection remain poorly understood but are critical for developing therapeutic strategies to prevent...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...protein is inefficient for pseudotyping; several modifications may enhance infectious particle production, ...particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations...

...ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a transcriptional...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

...Innovation (IPI). For these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage....cells and will interfere with conjugation. For applications such as these, the buffer can be removed by ...tissue panel in one of the antibody’s recommended applications. The specific QC experiments performed varies...order, ensuring each item ships safely and most economically. Actual shipping conditions will be shown in...

...compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents) ACP...-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...Specific labeling of cell surface proteins with chemically diverse compounds. J Am Chem Soc. 126(29):8896...

...advantages and disadvantages depending on the application. Firefly luciferase is considerably brighter ...luciferase-based plasmid systems for many different applications. Browse the Collection Highlights section to...use the red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared...respectively, for increased usefulness in in vivo applications. LuxSit-i : An artificial luciferase desined...Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 ...

...transcription on the gene expression level without genetically altering the target loci. Thus, CRISPRi is a ...Plasmid Description 44246 pdCas9-humanized A catalytically inactive, human codon-optimized Cas9 expression...expression plasmid 44247 pdCas9::BFP-humanized A catalytically inactive, human codon-optimized Cas9-BFP fusion...codon-optimized Cas9 constructs 44249 pdCas9-bacteria A catalytically inactive bacterial Cas9 expression plasmid 44250...

...both dividing and non-dividing cells without a significant immune response. These viruses also integrate...divided among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode...with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This table lists...included to monitor expression Parijs 11619 pLB 3rd Modification of pLL3.7; Genetic elements known to prevent...

...and Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish....upstream of a 5' NGG 3' PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR...Gene Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens...be added to this table from any peer reviewed publication. To add validated gRNA sequences to our datatable...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled... supplemental materials from the Doyon Lab’s publication: Dalvai et al. Cell Rep. 2015 . Alternatively...programming sgRNA and HR donors for selectable genome modification. This system can be used to create C- and N-...following articles provide PCR templates for amplification of the tag (eg GFP, Flag, YFP, etc) and selection...

...Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs.... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication...

...synthetic biology plasmids related to cell-to-cell communications and environmental sensing. Sensing and Signaling... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Gene/Insert Vector Type Promoter Tags PI Publication Do you have suggestions for other plasmids that...

... collection will grow as the IGVF community systematically perturbs the genome to assess the impact of...Addgene. ID Plasmid Description Vector Type PI Publication Additional Resources The IGVF Consortium (Link...opens in a new window) . Find IGVF Consortium publications on bioRxiv (Link opens in a new window) or PubMed...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor...systems. Mammalian ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that...

... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...used for screening transcription factors for applications such as cellular programming or cancer drug ...barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI Description...