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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 149 results for: ins

Showing: 61 - 80 of 149 results

  1. Retrovirus Plasmids

    Type
    Collection
    ...its genome and several structural and enzymatic proteins, including reverse transcriptase and integrase...genome, which can lead to oncogenesis (termed insertional mutagenesis). Read our γ-Retrovirus Guide for...Plasmid Type Description PI 8449 pUMVC Packaging Contains MoMLV gag and pol; use with envelope plasmid Weinberg... Weinberg 12371 pCL-Eco Packaging Contains MoMLV gag, pol, and env Verma 35614 pBS-CMV-gagpol Packaging...database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description PI 1780 pBABE-neo...modified version of pRX-tight Puro encoding NAT instead of PAC Iglehart 20672 MSCV-IRES-GFP MSCV For transgene...

  2. Antibody Production

    Type
    Collection
    ... distributed by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies...more about these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage...with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and then purified...opens in a new window) . Western Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE...thawing on ice, and then testing for loss of potency against a non-treated control in one of its recommended... Antibody Guide Antibody Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

  3. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table contains a general list of plasmids... handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can transduce both...AAV particles without the use of a helper virus. Instead, specific gene products can be provided by helper...All Available AAV Packaging Plasmids This table contains helper plasmids for packaging your AAV product..., species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have...

  4. Microbiology Resources

    Type
    Collection
    ...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial fields,...and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz...MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous...Vaccinia virus ORF library : Expression library that contains each of the predicted open reading frames for ... : 285 vectors for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB ...Restoration of prototrophy in common S. cerevisiae lab strains - Ralser Lab Return to top Resources for...

  5. Viral Vectors

    Type
    Collection
    ...depending on the insert type and envelope used. Please consult with your institution’s Biosafety Committee...that they no longer encode for more virus, but instead encode for a specific gene or other research interest...with specific genomes (Fig. 1A) . In other words, instead of a virus infecting a host and giving rise to ... same way that naturally occurring viruses do. Instead, the genes that are required for viral replication... be produced. Each of these plasmids, however, retains the properties of any typical plasmid and thus ...

  6. NETRF

    Type
    Collection
    ...Investigators (PIs) PI Institution NETRF Project Title Hans Clevers Hubrecht Institute Modeling Neuroendocrine...Organoids Matthew Meyerson Dana-Farber Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine...Neuroendocrine Tumors Ramesh Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine ... and Carcinoid Tumors Bradley Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers...

  7. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for many...Browse plasmids encoding SARS-CoV-2 viral proteins. Depositor...enable researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene has partnered...2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope, nsp3, OC43, NL63, HKU1, ...

  8. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins In Vivo Imaging Guide Fluorescent Proteins: In ...fluorescent proteins. Plasmid...protein interactions in living cells and live mice. Proteins for in vivo imaging have emission near or above... background signals. Near-infrared fluorescent proteins for in vivo imaging are derived from bacterial...

  9. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...boundary defined by anti-CRISPR proteins or running into other proteins bound to DNA, and the break can...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication...Publication Plant ID Plasmid Gene/Insert Promoter PI Publication Last reviewed on: January 30, 2025 Do you ...

  10. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable...Marker PI Publication Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you...

  11. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...They encourage instructors teaching different types of courses, at any type of institution, and at different...facilitates scientific collaborations between institutions. With these plasmids, faculty can begin a sustainable...subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community...External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP...

  12. AAVED

    Type
    Collection
    ...School Ali Cetin Allen Institute for Brain Science Ben Deverman Broad Institute Katherine Matho Cold Spring...Ali Cetin and Shenqin Yao, Allen Institute Ben Deverman, Broad Institute Katherine Matho, Cold Spring Harbor...meeting. The goal of this year’s workshop is to brainstorm practical strategies for using combinations of...

  13. AAV Packaged on Request

    Type
    Collection
    ...Additional steps, including request approval, your institution’s approval of the MTA, and shipping, mean that... typically takes 1–4 days, depending on your institution. AAV Production 6–8 weeks Quality is our priority...vector experience, from methods to troubleshooting insights, you make research more reproducible for scientists...information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources Viral Vector Production... that complement published data, capturing the insights that often go unshared. Help Center Check out ...

  14. Plant Plasmids and Resources

    Type
    Collection
    ...Backbones Fluorescent Proteins Other Resources Background Addgene’s repository contains many plasmid tools...Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent proteins for ...engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids containing...

  15. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...and pICPIS-CB. pICPIS-EF1 contains the EF1 promoter while pICPIS-CB contains the Chicken-beta actin promoter...plates — if your insert is successfully cloned into this vector, transformants will instead form white colonies...promoter pLL3.7-TNFa-shRNA3 72598 Expresses shRNA against mouse TNF-alpha mRNA from the mouse U6 promoter...

  16. Fluorescent Protein Guide: Activity Regulation

    Type
    Collection
    ...Plasmid Collections Fluorescent Proteins Activity Regulation Fluorescent Proteins: Activity Regulation More ...Biosensors Optogenetics Background Fluorescent proteins such as GFP have long been used for labeling, ... methods are emerging to use these fluorescent proteins to modulate biological activity. Early tools allow...

  17. Depositor Collections

    Type
    Collection
    ...- Davidson Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science ...Collections Depositor Collections Addgene's repository contains 159,238 plasmids, which includes plasmid collections...Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection...

  18. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable...

  19. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...recruit specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic...requires recruitment of many copies of labeled proteins to the given region. For example, chromosome-specific...throughout the chromosome. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you...

  20. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable...subsequently isolated by affinity purification against the epitope tag. Compared to conventional methods...Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you...
Showing: 61 - 80 of 149 results