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TypeCollection...Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range of microbial fields,...and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz...MultiSite Gateway Kit : Creating three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous...Vaccinia virus ORF library : Expression library that contains each of the predicted open reading frames for ... : 285 vectors for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB ...Restoration of prototrophy in common S. cerevisiae lab strains - Ralser Lab Return to top Resources for...
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Adeno-associated virus (AAV) Plasmids
TypeCollection...and more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table contains a general list of plasmids... handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can transduce both...AAV particles without the use of a helper virus. Instead, specific gene products can be provided by helper...All Available AAV Packaging Plasmids This table contains helper plasmids for packaging your AAV product..., species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI Do you have... -
Plant Plasmids and Resources
TypeCollection...Backbones Fluorescent Proteins Other Resources Background Addgene’s repository contains many plasmid tools...Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent proteins for ...engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids containing...fluorescent protein of choice. Visit our Fluorescent Proteins Guide for more. ID Plasmid Description Promoter... -
Tetracycline Inducible Expression
TypeCollection...blasticidin selection. *Note: this vector contains an insert (MAT2A) which can be replaced by another ...expression. Improved expression of the transactivator proteins in mammalian cells or other species by codon optimization...Lentiviral Tet-On vector for inducible expression; insert with Gateway cloning and puromycin selection rtTA-Advanced...Off) of both your gene of interest and EGFP. Pbi contains a TRE between two minimal CMV promoters. None ... CRISPRi. See Plasmid #167937 for CRISPRa Emma Rawlins 72835 pMK243 (Tet-OsTIR1-PURO) Expresses OsTIR1... of the G1/S phase transition by expression of cyclins D1 and E with an inducible system . Mol Cell Biol... -
NETRF
TypeCollection...Investigators (PIs) PI Institution NETRF Project Title Hans Clevers Hubrecht Institute Modeling Neuroendocrine...Organoids Matthew Meyerson Dana-Farber Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine...Neuroendocrine Tumors Ramesh Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine ... and Carcinoid Tumors Bradley Bernstein Broad Institute Identifying Altered Epigenetic States and Drivers... -
Ginkgo Bioworks COVID-19 Collection
TypeCollection...therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for many... Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems. Depositor...enable researchers to express SARS-CoV-2 viral proteins in several expression systems. Addgene partnered...2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope, nsp3, OC43, NL63, HKU1, ... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins: In Vivo...fluorescent proteins. Plasmid...whole mammals. Genetically encoded fluorescent proteins most suitable for stable imaging of protein interactions...background signals. Many near-infrared fluorescent proteins for in vivo imaging are derived from bacterial... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection...boundary defined by anti-CRISPR proteins or running into other proteins bound to DNA, and the break can...Plasmid Gene/Insert Promoter PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI ...Publication Return to top Plant ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources... -
CRISPR Plasmids - Base Edit
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return... -
Malate Dehydrogenase CUREs Community Collection
TypeCollection...They encourage instructors teaching different types of courses, at any type of institution, and at different...facilitates scientific collaborations between institutions. With these plasmids, faculty can begin a sustainable...subunit interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community...External Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP... -
E11 Bio PRISM Collection
TypeCollection...reconstruction of individual neurons in brain tissue. PRISM begins with the delivery of stochastic combinations of...al. (2025) use plasmids expressing the barcode proteins under the constitutive CAG promoter (Addgene #242764...include using individual or subsets of barcode proteins to label and detect multiple injection sites in...window) , in collaboration with The Francis Crick Institute (Link opens in a new window) , MIT (Link opens...opens in a new window) , and The Max Planck Institute for Biological Intelligence (Link opens in a new window... -
AAV Packaged on Request
TypeCollection...Additional steps, including request approval, your institution’s approval of the MTA, and shipping, mean that... typically takes 1–4 days, depending on your institution. AAV Production 6–8 weeks Quality is our priority...vector experience, from methods to troubleshooting insights, you make research more reproducible for scientists...information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources Viral Vector Production... that complement published data, capturing the insights that often go unshared. Help Center Check out ... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter... -
Depositor Collections
TypeCollection...Collection Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science ...Collections Depositor Collections Addgene's repository contains 165,025 plasmids, which includes plasmid collections...Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection...Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter...subsequently isolated by affinity purification against the epitope tag. Compared to conventional methods...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return... -
AAV Viral Preps
TypeCollection...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster, Brainbow Molecular Tools ... -
CRISPR Plasmids for Genomic Visualization
TypeCollection...recruit specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic...requires recruitment of many copies of labeled proteins to the given region. For example, chromosome-specific...read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return... -
CRISPR Plasmids - Parasites
TypeCollection...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma...knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes. Do you have... -
CRISPR Plasmids - C. elegans
TypeCollection...joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often...repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs...much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically ...activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression Vectors... -
Plasmids for Stem Cell Research
TypeCollection... L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations A more efficient method...regenerative medicine. iPSCs, much like their ES cell cousins, have the hallmarks of pluripotent cells--they ...Ottosson Stem Cell Research Plasmids This table contains a general list of plasmids that have been used...name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions...