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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 123 results for: tra

Showing: 61 - 80 of 123 results

  1. CRISPR Plasmids and Resources

    Type
    Collection
    ...generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. ...deletions. Insert NEW CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

  2. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...124606 SOX2-mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964...159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499...human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens...

  3. Neurodegeneration Research Collection

    Type
    Collection
    ...neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent species...delivery of viral particles that will target the central nervous system, peripheral nervous system, vesicular...kinetics and localization for imaging synaptic transmission. Aggarwal et al. Nat Methods. 2023 May 4. See...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. New...PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, and cortical neuron...Use a FRET-based method to monitor structural transitions in protein self-organization. Wan et al. Cell...

  4. Cancer Research Plasmids and Resources

    Type
    Collection
    ...mutations. Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers...kinase that anchors a signaling network for transitioning between anabolic and catabolic states. Ras Ras...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon...cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier...

  5. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...21871 pMLM36 (aka pBR-UV5-GP-FD2) 21874 Strain KJ1C 21875 Strain CSH100 Content blocked, you may need to... Keith Joung This kit consists of plasmids and strains used to engineer ZFNs via the Oligomerized Pool... packaged plasmids and 3 individually packaged strains, which are shipped as bacterial stabs at room temperature... pAC-Kan-alphaGal4 13422 pBAC-lacZ 13424 KJBAC1 strain 21869 pKJ1267 (aka pAC-alphaGal4) 21870 pKJ1712...

  6. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...understood but are critical for developing therapeutic strategies to prevent viral spread. As a Biosafety Level...mechanisms of infection and to determine methods of neutralizing the virus or reducing its infective potential...that can be used for measuring infectivity and neutralizing activity are listed below: ID Plasmid Description...provirus and the overall strength of viral transcriptional response in these cells. Warner Greene 105621...Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV...

  7. Synthetic Biology - Overview

    Type
    Collection
    ...sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain functional...Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard...and pOSIP Plasmid Kit Gray Lab Maize and Rice Transcription Factors Hasty Lab Biopixels Jaramillo Lab Riboregulation...

  8. Distribution to Industry

    Type
    Collection
    ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

  9. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian U6...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...

  10. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Addgene AAV transfer plasmids...or toxins should be handled at BSL-2. AAV can transduce both dividing and non-dividing cells with a low... AAV does not integrate into the host genome, transgene expression can be long-lived. The utility of AAV...effort directed toward expanding this capacity. Traditionally, AAV requires the presence of another "helper...viral genome (typically called cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description...

  11. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina...Inhibition Mouse Weissman 3rd 5 Varies Human CRISPRi titration libraries 136478 & 136479 Inhibition Human Weissman...Knockout Human Superti-Furga 3rd 6 2,609 Human Transporter Knockout Libraries 213695 221409 221410 Knockout...Human Superti-Furga 3rd Varies Varies Human Transcription Factor Knockout Library 162275 Knockout Human...Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library 138015 Knockout...

  12. CRISPR References and Information

    Type
    Collection
    ...are human (hg19 & hg38), mouse (mm10), and yeast (strain w303). Developed by Venetia Pliatsika and Isidore...and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9...105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression...and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49 KB Vosshall...RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF, 255 KB...

  13. Brain Initiative Collection

    Type
    Collection
    ...pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent...

  14. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250...42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other...

  15. Optogenetics Plasmid Collection

    Type
    Collection
    ... been engineered or identified to increase the spectral and kinetic properties as well as improve the ... a fluorescent protein to the opsin in order to track its expression and localization. Many other types...For a full description (including peak response spectra) of different types of opsins and optical switches...cellular activity. For example, search "Cdc42", "transcription", or other terms. ID Plasmid Description Gene...

  16. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation can continue ...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...

  17. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP concentration given relatively... S. (2018). Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial... genes without cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet...

  18. TALEN Engineering

    Type
    Collection
    ...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

  19. Resolute Plasmid Collection

    Type
    Collection
    ...CRISPR knockout library targeting human transmembrane transporters. Feedback Please send your feedback to...carrier proteins (SLCs) are the largest family of transporters in the human genome with approximately 450 identified...overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 221409 221410 Superti-Furga...

  20. Antibody Plasmid Collection

    Type
    Collection
    ...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...is present in a sample — just to name a few. Traditional sources of antibodies for research purposes include...single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. ...
Showing: 61 - 80 of 123 results