We narrowed to 112 results for: tra

...understood but are critical for developing therapeutic strategies to prevent viral spread. As a Biosafety Level...mechanisms of infection and to determine methods of neutralizing the virus or reducing its infective potential...that can be used for measuring infectivity and neutralizing activity are listed below: ID Plasmid Description...provirus and the overall strength of viral transcriptional response in these cells. Warner Greene 105621...Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV...

...sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain functional...Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard...and pOSIP Plasmid Kit Gray Lab Maize and Rice Transcription Factors Hasty Lab Biopixels Jaramillo Lab Riboregulation...

... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

...are human (hg19 & hg38), mouse (mm10), and yeast (strain w303). Developed by Venetia Pliatsika and Isidore...and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9...105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression...and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF, 49 KB Vosshall...RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF, 255 KB...

...pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent...

... been engineered or identified to increase the spectral and kinetic properties as well as improve the ... a fluorescent protein to the opsin in order to track its expression and localization. Many other types...For a full description (including peak response spectra) of different types of opsins and optical switches...cellular activity. For example, search "Cdc42", "transcription", or other terms. ID Plasmid Description Gene...

...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation can continue ...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...

...also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP concentration given relatively... S. (2018). Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial... genes without cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet...

...Hockemeyer et al., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., ...identify both dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the ...sequences of interest for potential TALEN or TALE transcriptional activator target sites and then generates individual...Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast...

...CRISPR knockout library targeting human transmembrane transporters. Feedback Please send your feedback to...carrier proteins (SLCs) are the largest family of transporters in the human genome with approximately 450 identified...overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 221409 221410 Superti-Furga...

...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...is present in a sample — just to name a few. Traditional sources of antibodies for research purposes include...single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. ...

... mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin...Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated...Neurofibromin 1 PRAS40 Also known as AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase and tensin homolog....2014.01.001. PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea...

...plasmids is a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and ... Integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica ...Restoration of prototrophy in common S. cerevisiae lab strains - Ralser Lab Return to top Resources for... in bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome...

... used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name...Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic...

...this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard viral...serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...

...plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique ...

...CRISPR plasmids for transcriptional repression of target genes....Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. This interference is most efficient when the gRNA...

...technical reports from the research community demonstrating AAV performance under various experimental conditions... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives...

...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...

... other difficult-to-target antigens that are intractable via animal immunization. IPI is a nonprofit research...researchers with powerful tools to investigate neurotransmission, synapse formation, neural connectivity, plasticity... top Integrins Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit...