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Showing: 61 - 80 of 130 results

  1. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...may yield slightly different DNA concentrations. Can I directly transfect cloning grade DNA into cells? ...experiments by removing the amplification and extraction steps required when one receives plasmids in ...DNA Service Details Amount 2 µg Volume 20 µl Concentration 100 ng/µl (± 5ng/µl) Buffer 10 mM Tris-Cl, pH...validated by next generation sequencing. The DNA concentration and 260/280 ratio has been determined by spectrophotometer...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...

  2. CRISPR Plasmids and Resources

    Type
    Collection
    ...generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand DNA breaks. ...deletions. Insert NEW CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9...Prime Edit Cas nickase enzymes fused to reverse transcriptase install new genetic information into a specific..., or fused to a repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier...

  3. Synthetic Biology - Overview

    Type
    Collection
    ...sensing. Strains Browse Addgene's collection of engineered bacterial strains, including plasmid-strain functional...Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard...and pOSIP Plasmid Kit Gray Lab Maize and Rice Transcription Factors Hasty Lab Biopixels Jaramillo Lab Riboregulation...Synthetic Biology Articles - Peccoud lab social media tracker Synthetic Biology - Open access journal Do you ...

  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...124606 SOX2-mEGFP AICSDP-60 mEGFP Transcription factor SOX-2 Transcription Factor 124607 ACTN2-mEGFP AICSDP...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...133963 UBTF-HaloTag AICSDP-80 HaloTag Nucleolar transcription factor UBF Nucleolus (fibrillar center) 133964...159745 TFAM-mEGFP AICSDP-72 mEGFP Mitochondrial transcription factor A (TFAM) Mitochondrial nucleoids 164499...human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells (Link opens...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ...neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent species...delivery of viral particles that will target the central nervous system, peripheral nervous system, vesicular...kinetics and localization for imaging synaptic transmission. Aggarwal et al. Nat Methods. 2023 May 4. See...Differentiation Factors Find plasmids used to transform iPSCs and fibroblasts into neurons and more. New...PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, and cortical neuron...Use a FRET-based method to monitor structural transitions in protein self-organization. Wan et al. Cell...

  6. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ...CRISPR plasmids for transcriptional repression of target genes....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression...

  7. Cancer Research Plasmids and Resources

    Type
    Collection
    ...mutations. Resources New Tool for Lineage Tracing: The ClonTracer Library (Frank Stegmeier) Tackling Cancers...kinase that anchors a signaling network for transitioning between anabolic and catabolic states. Ras Ras...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon...cDNAs for studying oncogenic signaling pathways. ClonTracer Library : Barcoding library from the Stegmeier...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...non-targeted strand, Cas3 starts unraveling DNA using its helicase properties, cleaving the targeted strand non-specifically...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...After nicking the target DNA, Cas3 initiates single-strand DNA degradation. This degradation can continue ...non-specifically along the way. Cas3 initiates a final double-strand break, either due to a boundary defined by anti-CRISPR...

  9. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...21871 pMLM36 (aka pBR-UV5-GP-FD2) 21874 Strain KJ1C 21875 Strain CSH100 Content blocked, you may need to... Keith Joung This kit consists of plasmids and strains used to engineer ZFNs via the Oligomerized Pool... packaged plasmids and 3 individually packaged strains, which are shipped as bacterial stabs at room temperature... pAC-Kan-alphaGal4 13422 pBAC-lacZ 13424 KJBAC1 strain 21869 pKJ1267 (aka pAC-alphaGal4) 21870 pKJ1712...

  10. Distribution to Industry

    Type
    Collection
    ... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

  11. AAVED

    Type
    Collection
    ...AM AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan 11:00 AM Circuit Tracing Ali Cetin 12:00 PM...this year’s workshop is to brainstorm practical strategies for using combinations of AAV-based molecular...Ben Deverman 2:00 PM Cre-DOG and Combinatorial Strategies Connie Cepko 2:45 PM Break 3:00 PM (Group Discussion...of data and protocols showing examples of AAV strategies for intersectional control. Please see our list...

  12. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Addgene AAV transfer plasmids...or toxins should be handled at BSL-2. AAV can transduce both dividing and non-dividing cells with a low... AAV does not integrate into the host genome, transgene expression can be long-lived. The utility of AAV...effort directed toward expanding this capacity. Traditionally, AAV requires the presence of another "helper...viral genome (typically called cis -plasmid or transfer plasmid). Helper Plasmid ID Plasmid Serotype Description...

  13. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...in vitro transcription BsaI none S. pyogenes Mark pU6T7 71462 Mammalian/in vitro transcription BsaI none...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian U6...toolbox for multiplexed plant genome editing and transcriptional regulation Plant Three types of plasmids available...recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs Zebrafish Plasmids...multiple functional gRNAs from a single precursor transcript Bullock pCRISPathBrick Bacteria Allows rapid ...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Human Bonifacino 3rd 6-25 11,108 Borodina Yeast Transporter Libraries 153101-153106 Knockout Yeast Borodina...Inhibition Mouse Weissman 3rd 5 Varies Human CRISPRi titration libraries 136478 & 136479 Inhibition Human Weissman...Knockout Human Superti-Furga 3rd 6 2,609 Human Transporter Knockout Libraries 213695 221409 221410 Knockout...Human Superti-Furga 3rd Varies Varies Human Transcription Factor Knockout Library 162275 Knockout Human...Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library 138015 Knockout...

  15. CRISPR References and Information

    Type
    Collection
    ...are human (hg19 & hg38), mouse (mm10), and yeast (strain w303). CRISPR MultiTargeter Can be used to identify...and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9...102.5 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression...and BsmBI site for cloning in gRNA: pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) PDF 47.5 KB Vosshall...RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) ; PDF 248.6...

  16. Brain Initiative Collection

    Type
    Collection
    ...pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent...

  17. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression...

  18. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined...PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription none, need Cas9 plasmid Chen and Wente 42250...42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other...

  19. Optogenetics Plasmid Collection

    Type
    Collection
    ... been engineered or identified to increase the spectral and kinetic properties as well as improve the ... a fluorescent protein to the opsin in order to track its expression and localization. Many other types...For a full description (including peak response spectra) of different types of opsins and optical switches...cellular activity. For example, search "Cdc42", "transcription", "Hahn", or other terms. ID Plasmid Description...

  20. FlyCRISPR

    Type
    Collection
    ...in vivo transcrition controlled by the regulatory sequences of an RNA polymerase III transcribed U6 snRNA...can be efficiently generated in Drosophila and transmitted through the germline. Using an injection approach... : Generates crRNA for use in combination with tracrRNA. 51434 pHD-DsRed : vector for generating dsDNA...
Showing: 61 - 80 of 130 results