We narrowed to 132 results for: Cat
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TypeCollection...bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate, leading to the emission...advantages and disadvantages depending on the application. Firefly luciferase is considerably brighter ...luciferase-based plasmid systems for many different applications. Browse the Collection Highlights section to...respectively, for increased usefulness in in vivo applications. LuxSit-i : An artificial luciferase desined...
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Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection... has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled...used for screening transcription factors for applications such as cellular programming or cancer drug ...barcoded with a 24 bp sequence, to simplify identification during screening. Library ID PI Description... -
CRISPR Plasmids for Genomic Visualization
TypeCollection...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically inactive Cas9 (dCas9) fused to a fluorescent...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that... -
CRISPR Plasmids - Parasites
TypeCollection...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs ... ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled Library... -
DNA Service - Cloning Grade DNA
TypeCollection...reducing the time to experiments by removing the amplification and extraction steps required when one receives...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended... -
Zinc Finger Consortium: OPEN Reagents
TypeCollection...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...vesicular stomatitis virus G) are packaged as replication-defective virus particles with the SARS-CoV-2...protein is inefficient for pseudotyping; several modifications may enhance infectious particle production, ...particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description Mutations... -
Addgene Packaged on Request: Scope of Service
TypeCollection...without a guaranteed titer. Any titer expectations communicated by email will supersede the general guarantees...step of the QC process, viral genome sequence verification, may not yet have been completed at the time...longer turnaround time. Any timing expectations communicated by email will supersede the general estimated... -
Synthetic Biology - Overview
TypeCollection... by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including... , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches Other Addgene Tools and... -
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...compatible with multiple applications (different fluorophores, biotin, bead purification, blocking agents) ACP...formation in nonribosomal peptide biosynthesis. Catalytic role of the condensation domain. J Biol Chem. ... -
Plant Plasmids and Resources
TypeCollection...Multipurpose Toolkit enables targeted, specific modification of monocot and dicot genomes using genome engineering...engineering; validated in tomato, tobacco, M. truncatula , wheat, and barley - Daniel Voytas. A CRISPR...Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature... -
Open Enzyme Collection
TypeCollection...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...then transform into E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended... -
Viral Production
TypeCollection...minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic properties...posted on the material page for the corresponding catalog item (see maps section for images). Figure 2: AAV...typically prepared using endotoxin-free plasmid purification kits. Resources Browse lentiviral preps available... -
Synthetic Biology - Algal
TypeCollection... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that... -
Synthetic Biology - Bacterial
TypeCollection... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that... -
Synthetic Biology - Fungal
TypeCollection... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that... -
Synthetic Biology - Mammalian
TypeCollection... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that... -
Synthetic Biology - Plant
TypeCollection... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that... -
Synthetic Biology - Worm
TypeCollection... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that... -
Synthetic Biology - Yeast
TypeCollection... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...