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We narrowed to 152 results for: LAS

Showing: 81 - 100 of 152 results

  1. TALEN Engineering

    Type
    Collection
    ... like... Keith Joung Lab plasmids Daniel Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed...construction of TALEN expression plasmids using REAL, REAL-Fast or FLASH assembly. This new version of ZiFiT...REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH assembly. Links to additional pages...Individual REAL TALE Repeat Plasmids TALEN Expression Vectors for REAL, REAL-Fast and FLASH TALE Transcriptional...Gene Additional Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome ...TALENs, and assembly methods for REAL, REAL-Fast and FLASH Genome...method (Reyon et al., manuscript submitted), and the FLASH (Fast Ligation-based Automatable Solid-phase High-throughput...

  2. Distribution to Industry

    Type
    Collection
    ...collection of plasmids that are available to industry. Learn more about the available plasmids and how to...Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled Libraries Antibodies Ordering ...type, species, and more. Plasmids Browse the table below to see all of the plasmids that are currently available...Biolabs Cancer Research from Sinai Health System Plasmids from the Structural Genomics Consortium Solute...Foundation Search Use Addgene's Search to find plasmids available to industry scientists while also adding...available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity...Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning and assembling standardized parts ...

  3. Genetic Code Expansion

    Type
    Collection
    ...Find GCE synthetase plasmids, target plasmids, and bacterial strains useful for expanding the genetic... Plasmid Collections Genetic Code Expansion Genetic Code Expansion Synthetase Plasmids Target Plasmids...achieve these modifications, two plasmids are generally used. Once these plasmids have been introduced in the... ncAAs. This plasmid often carries multiple tRNA copies for efficiency. A target plasmid containing the...NIH-funded GCE4All Research Center . Synthetase Plasmids Browse plasmids that contain aminoacyl-tRNA synthetases... of these plasmids also contain one or multiple copies of the cognate tRNA gene. ID Plasmid Synthetase...Bacterial TAG Wei Niu Return to top Target Plasmids Browse plasmids that contain genes with modified codons...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids References ...novel INTSRECT plasmids for groups interested in producing their own INTRSECT plasmids. Online spreadsheet...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...that have used these constructs experimentally. Plasmids In addition to EYFP and ChR2-EYFP, a large number...rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items ...

  5. Antibody Guide

    Type
    Collection
    ...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic... genes from specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...Isotypes The mammalian immune system produces five classes of antibodies, known as isotypes. An isotype is...antibodies can be isotype-specific or even isotype subclass-specific. This specificity can be used to ensure..., while others exist as monomers. Each antibody class therefore has a valency, or number of binding sites...

  6. Synthetic Biology - Overview

    Type
    Collection
    ...process . Browse SynBio Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning ...bacterial strains, including plasmid-strain functional pairs. Browse Plasmids by Model Organism Bacterial...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail... Plasmid Collections Synthetic Biology Synthetic Biology...Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas Buchler George Church Cynthia Collins James Collins...Addgene can help you to share your synthetic biology plasmids with the community. To deposit, view our SynBio...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...

  7. Fluorescent Protein Guide

    Type
    Collection
    ...Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's plasmid repository contains thousands ...proteins and to find plasmids for your next experiment. Browse Fluorescent Protein Plasmids by Category Empty...Proteins Fluorescent Protein Plasmid Kits Allen Institute for Cell Science Plasmid Collection New England BioLabs... BioLabs Cell-Imaging Plasmid Collection Promega Plasmid Collection Botman-Teusink Yeast Fluorescent Protein...and to find the plasmids that are available from Addgene's depositing scientists. Plasmid... Plasmid Collections Fluorescent...Resources at Addgene Optogenetics Guide | Optogenetics Plasmids Use light to activate, inhibit, and control molecular...

  8. Lentiviral Prep Service

    Type
    Collection
    ...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes...2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding...processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert... pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 ...

  9. CRISPR References and Information

    Type
    Collection
    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE...(Link opens in a new window) An encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning...

  10. Viral Production

    Type
    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...Control Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination... tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...contamination is minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic...contamination using mycoplasma detection kits. The cell line is maintained for ~20 passages before being...off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching system...

  11. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...to view all plasmids available from the article. Click on the PI link to view all plasmids available from... all other plasmids from that article. Click on the PI link to view all available plasmids from this ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Synthetic Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning...Browse Addgene's collection of synthetic biology plasmids related to Cloning and Genomic Tools for library...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table...

  12. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ... more. Empty Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return...Return to top Cyan Plasmid Description Industry Return to top Green Plasmid Description Industry Return...Return to top mEmerald Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return...Return to top Orange Plasmid Description Industry Return to top Red Plasmid Description Industry Return... fluorescent proteins. Plasmid Description Industry Return to top Content last reviewed: 25 September ... Plasmid Collections Fluorescent Proteins Michael Davidson...proteins with your gene of interest. Most of these plasmids are available to industry scientists as well as...

  13. AAV Packaged on Request

    Type
    Collection
    ...transfer plasmids in our repository are eligible for this service. Select any eligible plasmid in the Addgene...applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the providing...real-world troubleshooting. Eligible Plasmids The following plasmids are eligible for this service. Submit...paper of your chosen AAV plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter ...Overview Details Ordering Share Your Results Eligible Plasmids Additional Resources Adeno-associated virus (AAV...lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven serotypes...important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

  14. Retrograde AAV viral preps

    Type
    Collection
    ...Calcium sensor Douglas Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Douglas Kim 104491 ...Calcium sensor Douglas Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE Syn GCaMP7f Calcium sensor Douglas Kim 104492 ...preparations were produced with the rAAV2-retro helper plasmid (Addgene #81070) . This serotype permits robust...transgene delivery to projection neurons. Specific classes of projection neurons can be targeted by using ...SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver staining,...pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 Syn Cre-dependent jRGECO1a Calcium Sensor Douglas Kim , GENIE Project 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40...pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn jRGECO1a Calcium Sensor Douglas Kim , GENIE Project 104487 pGP-AAV-syn-jGCaMP7s-WPRE...

  15. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Addgene Plasmids Bas Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources...Find plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent...codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows for constitutive overexpression of FPs ...proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al., 2004; Lee et al., 2013), and are...codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter...promoter and the URA3 marker, the p415 plasmid (Loqué et al., 2007) containing the TEF1 promoter and the LEU2...

  16. Plan Your Experiment

    Type
    Collection
    ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...Application Cas Enzyme Additional Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells...different activator systems exist, including the multi-plasmid SAM system Activate Design and Clone Your gRNA ..., anneal, and insert the targeting oligos into plasmids containing the gRNA scaffold using standard restriction-ligation...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene ...the expression time (transient or stable). For plasmid and viral-based approaches, the species and expression...fluorophore) are also relevant. Delivery Using Plasmids The most straightforward method to deliver a CRISPR...

  17. Ras Pathway

    Type
    Collection
    ...human cancers. Ras Pathway Plasmids Click on a name to find available plasmids for the gene, or browse the...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene's collection of plasmids for the Ras pathway. Ras is a small GTPase and constitutively active...mTOR Pathway p53 Pathway Background Ras Pathway Plasmids Ras Gene List Resources Background Ras is a small...Schematic of Ras signaling cascade that starts at the plasma membrane. Cancer Impact Ras is ubiquitously expressed... - Gene List Click on a name to find available plasmids for the gene. For components with multiple subunits...v-akt murine thymoma viral oncogene homolog 3 ALK Anaplastic lymphoma receptor tyrosine kinase ARHGAP35 Rho...

  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References... Antibody Plasmid Collection NeuroMab Plasmid Collection...distinct IgG subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary... the plasmid, followed by the SV40 polyA tail (SV40 pA). Recombinant Monoclonal Antibody Plasmid Collection...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target...Mouse IgG2a 114542 Anti-LRP4 (cytoplasmic) [N295B/66R] LRP4 (cytoplasmic) Mouse Mouse IgG2a 114544 Anti-GABA...Mouse IgG2a 177555 Anti-ADAM22 (cytoplasmic) [N46/30R] ADAM22 (cytoplasmic) Mouse Mouse IgG2a 177556 Anti-Rufy3...

  19. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ... entity, please see our Plasmid Distribution to Industry page. These plasmids have not been expressed ...Addgene. Plasmids Use the search bar or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based...SARS-CoV-2 Plasmids for Many Expression Systems Collection: COVID-19 and Coronavirus Plasmids & Resources...Browse Ginkgo Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems. Depositor...Ginkgo Bioworks COVID-19 Ginkgo Bioworks COVID-19 Plasmid Collection (Link opens in a new window) Researchers...systems. Addgene partnered with Ginkgo to make these plasmids available to scientists in academic and industrial...) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional ...

  20. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Packaged on Request AAV Guide Plasmids Publications Enhancer AAV plasmids are plasmids which use genomic regulatory... Tools Atlas (Link opens in a new window) . Allen Institute for Brain Science Enhancer Plasmids The Allen...Science Enhancer Collection contains best-in-class AAV plasmids to target a variety of cell populations in...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations...details, visit the Allen Institute's Genetic Tools Atlas (Link opens in a new window) . Figure 1: Selection...tested in mouse brains. This collection contains plasmids for cell populations in the following regions:...Hypothalamus Cerebellum Whole brain Addgene ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled...
Showing: 81 - 100 of 152 results