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...Depositing Labs Juan Belmonte Linzhao Cheng Jose Cibelli George Daley Jin-Tang Dong Steven Dowdy Scott Friedman...regulators with diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism...

...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...threonine-specific protein kinases. They regulate diverse cellular programs by acting as an integration point for...

... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...Tetracycline Transactivators, Affinity Purification, Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies...

...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...

...Can I directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning...cloning applications. Direct transfection into cells is not recommended. Can I order more than 2 µg of cloning...

...microscopy is a powerful tool to study proteins and cells, but imaging in deep-tissue organs or whole animals...Proteins: Empty Backbones Fluorescent Proteins: Subcellular Localization Luciferase Plasmid Collection Viral...

...Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm ...Consortium ARF GTPase Family BRAIN Initiative Collection Cell Migration Consortium Impact of Genomic Variation...

...allowing a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic...engineered receptor is coupled to and the specific cell type where the receptor is expressed, these receptors...

... inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine ...hypermutation) in a wider editing window, and are thus well suited to directed evolution applications. Examples...

...activation activity of three-fold or greater in a well-characterized bacterial two-hybrid reporter system...efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed PMID 18657511...

... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...distributed preps around the globe and shared our well-developed and trusted protocols and peer-reviewed...

...Maizel 1000000151 CRISPR-Cas9 Gateway System for Physcomitrella patens System for genome editing of the moss...arbuscular mycorrhiza-inducible promoters to label organelles, membranes and cytoskeletal elements in M. truncatula...

...therapies for brain disorders by enabling region- and cell-specific gene-delivery in the mouse brain through...Bonaguro, R. J., Brauer, E., Candido, T. R., Castellarin, M., Chen, J., Chen, Y., Cheng, J. C., Chopra...

...application-specific validation and quality control by Addgene as well as by our trusted partner labs. To learn more about...

...emerging targets in AD and creating high-quality, well-characterized lead molecules for prioritized targets...

... time and are potentially heritable in dividing cells. CRISPR activation and interference technologies...

...developments with CRISPR-Cas9 gene editing have propelled both reverse and forward genetics of these parasites...

... PA, and biotin tags, can be used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9...

...application-specific validation and quality control by Addgene as well as by our trusted partner labs. On this page you...

...bound to DNA, and the break can be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the...