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We narrowed to 94 results for: expression vector

Showing: 81 - 94 of 94 results

  1. University of Florida Serotype Testing Panel for the Eye and Brain

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    Collection
    ...full sequencing of the final viral vector preparation. These viral vector preparations were produced with...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep... Viral Vector Packaging Service AAV University of Florida Serotype...Serotype Testing Panel for the Eye and Brain Viral Vector Packaging Service: University of Florida Serotype...Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part of our Viral Service , Addgene is producing...of our standard viral production , these viral vectors undergo quality control, including AAV titration... in vitro as well as in vivo delivery where the vector is diluted by the physiologic body fluids, such...

  2. CRISPR Guide

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    Collection
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

  3. CRISPR Plasmids - Single-Strand Break (Nick)

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    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ... nickase plasmids. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...

  4. Ras Pathway

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    Collection
    ...to permit construction of RAS pathway gene expression vectors for in vitro and in vivo research. The RAS...plasma membrane. Cancer Impact Ras is ubiquitously expressed in all cells and all tissues. Ras can activate...Resources RAS Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway®...

  5. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...

  6. CRISPR Plasmids - Parasites

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    Collection
    ...CRISPR expression plasmids that have been designed for use in parasites....-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...

  7. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...to introduce a DSB. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...

  8. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Transposase plasmids. Plasmids are available for expression in mammalian systems and bacteria. Mammalian ...

  9. Control AAV Preps

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    Collection
    ... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control...Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  10. Genetic Code Expansion

    Type
    Collection
    ...plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase...codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place...with a different amino acid. They are typically expressed from a single plasmid, with multiple copies of...make sure that these conditions are true. Always express first with a control reporter gene – GFP for E....mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media... tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine...mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic...

  11. Allen Institute for Brain Science AAV Enhancer Collection

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    Collection
    ...termed enhancers — to drive cell type-specific expression. The Allen Institute for Brain Sciences has screened... AAV delivery. The collection includes the top vectors with the highest specificity and brightness, assessed...Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell Rep. 2021...

  12. Biosensor AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...Biosensor AAV Browse our AAV that express fluorescent biosensor proteins, including calcium sensors, under... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  13. Resolute Plasmid Collection

    Type
    Collection
    ... SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors allow a fast and efficient sub-cloning...Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression pooled .... Human SLC Overexpression Library 213694 Superti-Furga Inducible barcoded overexpression library of human...

  14. Immunology Research Plasmids and Resources

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    Collection
    ...® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate the immune...regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated ankyrin-containing...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building...selection cassettes and tools for inducible gene expression. Myristoylated Kinase Kit : Consists of 190 human...
Showing: 81 - 94 of 94 results