...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically inactive Cas9 (dCas9) fused to a fluorescent...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

...constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct...Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F Catalytically inactive PA-Cre CMV Mammalian Sato 123128 pET-His-Cre...excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre... this subtype of loxP plasmids, reporter genes indicate which cells have undergone Cre recombination. ...which reporters are initially OFF and then probabilistically ON or OFF following Cre recombination to maximize...

...reducing the time to experiments by removing the amplification and extraction steps required when one receives...determined by spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations...Cloning grade DNA is designed for use in cloning applications. Direct transfection into cells is not recommended...

...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

...soluble recombinant molecules. The proteins are typically used in systematic protein interaction screens...benchmarked protein microarray-based platform for the identification of novel low-affinity extracellular protein...Sun Y, Gallagher-Jones M, Barker C, Wright GJ. Analytical Biochem. 2012 May 1;424(1):45-53....

...Scientists using these zinc finger arrays in a publication should notify [email protected] and...acknowledge NIH grant number R01 GM088040 in the publication. References Rapid 'open-source' engineering of...zinc-finger nucleases for highly efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. ...

...not indicate a specific relationship. p53 Pathway Color is used for clarity and does not indicate a specific... of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury MP, Bourdon JC. ...

...libraries to screen for genes involved in specific biological processes. For more information, see our Guide...Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human ... Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused to a transactivator...

...polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription of RNA into DNA, and ...for fixing damaged and degraded DNA prior to amplification, enzymes for cutting protein sequences to remove...then transform into E. coli for expression and purification ( pET28a-LIC or other pET vectors are recommended...

...addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources Browse...minimized by using an endotoxin-free plasmid purification protocol. To minimize the immunogenic properties...

...(yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons Jonathan Ting Viviana Gradinaru...protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. Jonathan Ting.... Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru 163505-PHPeB...

... removal of a phosphate group. Under normal physiological conditions GTPases play prominent roles in signaling... below. Color is used for clarity and does not indicate a specific relationship. The content and map for...Research Color is used for clarity and does not indicate a specific relationship. The content and map for...

...Lentiviral, miRNA expression (PRIME) system for application in knockdown of gene expression at a single copy... shRNA cloning vector; for evaluation of shRNA efficacy using fluorescence; use with pGFPns-reporter for...Of mice and models: improved animal models for biomedical research. Bockamp E, Maringer M, Spangenberg ...

... enzyme that cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains...Andrew Ellington's lab. High-surety isothermal amplification and detection of SARS-CoV-2, including with ...Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor . FURIN - Andersen,...

...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...important variables include: Type of genetic modification - CRISPR libraries vary based on whether they...includes information about the type of genetic modification and target species. Additional information about...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...

... or sort by the table headings. Click on the publication link to view all plasmids available from the ...Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that...