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...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in mammalian systems. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in C. elegans. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a large selection...in Xenopus. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...California, San Francisco Browse Plasmids ID Plasmid Gene/Insert Vector Type Backbone Mutations Tags PI...between the Cell Migration Consortium (CMC) and Addgene, which highlights CMC depositing labs and plasmids...collaboration between the Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium...plasmids useful for cell migration research at Addgene. CMC investigators who have deposited plasmids ...

...Plasmid Gene/Insert Vector Type Promoter PI Publication Return to top CRISPR Resources Addgene has a large...zebrafish. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...tags for you to create fusion proteins with your gene of interest. Most of these plasmids are available...

...Mammalian ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

... pLEG/pREG Modular Viral Vector System - Dankort Gene Vector Core Viral Vectors - Oka...Depositor Plasmid Collections Depositor Collections Addgene's repository contains 167,150 plasmids, which includes...Michael J. Fox Foundation Plasmid Resource iPSC Neurodegenerative Disease Initiative (iNDI) Collection Rett ...expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection Screening Multiplexed...

...SARS-CoV-2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope, nsp3, OC43, NL63, ...Industry Additional Addgene Resources Blog: Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids... viral proteins in several expression systems. Addgene partnered with Ginkgo to make these plasmids available...expressed or functionally validated by Ginkgo or Addgene. Plasmids Use the search bar or sort buttons to...

...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively transduce many mammalian...Packaging Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse constructs...preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality control process...shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request service. You can choose...

...markers of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...cytoskeletal elements. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 26722 GFP-cortactin...cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 71367 Human Beta-catenin...pathways and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 11546 EGFP-LC3 Autophagosomes...Fluorescent Proteins: Subcellular Localization General Cytoskeleton Junctions Membrane Trafficking Additional...cytoskeleton remodeling or blocked membrane traffic. Addgene has assembled a collection of popular fluorescent...your protein is targeted to the same structure. General Structures Eukaryotic cells contain a variety of...

... of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression...engineering forms the core of the deorphanizing engine. Genetic and proteomic interactions represent the framework.... Protein production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded...integrated knowledgebase that features, among others, genetic variants and disease associations. Plasmids This...overexpression pooled libraries are useful tools for genetic screening experiments. These libraries target the...Plasmid Collection NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning Content last reviewed: 12...

...an empty backbone can be repeated to ligate your gene of interest after the promoter, and so on. The following...

...-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions ...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies...Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...

...currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High...Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to...protection for material providers. Addgene's deposit agreements allow Addgene to distribute plasmids on behalf...can be submitted to Addgene through your user account or by email to mta@addgene.org . After we review ...A portion of Addgene's collection is available to qualified for-profit entities. Addgene reserves the ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available...assembling standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors Kit Fluorescent ...

...site-specific recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page for more...236288 pAAV.TBG.PI.FLPo.rBG TBG none 8 Melina Fan , Addgene Research Program Light-Inducible Recombinases ID...

...opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid...definitions (e.g. neurons defined by a single gene or projection). Intersectional expression approaches ... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions...cells based on a doubly-specified combination of genetic and/or anatomical-defined parameters, by placing...Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT recombinases...pAAV-CaMKIIa-Flpo None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...Vcre No Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...

... disorders by enabling region- and cell-specific gene-delivery in the mouse brain through these human ...List of Pleiades MiniPromoters MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A pEMS1142 EGFP/NLS...

...fluorescent biosensors to measure biomolecules or genes via FRET or other assays....specific biomolecules, the activity of specific genes and cellular processes, or other factors like environmental...mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial content, structure, stress and...for a sensor for a different analyte, a sensor for gene-specific activity, or for popular biosensors in ...mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible for Viral Packaging ...Gadella Calcium Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca 2+ indicator...WHaloCaMP1 chemigenetic calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium...