We narrowed to 24 results for: Apple fluorescent protein

... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's...Study your protein of interest by fusing it to a fluorescent protein. Michael Davidson Fluorescent Protein...More and Explore Addgene Blog: Fluorescent Proteins Fluorescent Protein Plasmid Kits Allen Institute for...fluorescent proteins and to find plasmids for your next experiment. Browse Fluorescent Protein Plasmids ...standards to study protein-protein interactions or conformational changes within a protein. Biosensors Monitor...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that are...thousands of fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent...

...Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal ...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP...pH-sensitive fluorescent protein pHmScarlet . pHmScarlet is a pH-sensitive red fluorescent protein to monitor...Additional Resources Fluorescent biosensors are proteins designed so that their fluorescent properties are ...signal of a circularly permuted fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET...orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer Calcium Orange fluorescent calcium...Glucose Green fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based Glucose...

...Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein Interactions Förster...Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization... Split Fluorescent Proteins for Studying Protein–Protein Interactions Return to top Controlled Expression...that can be used for protein purification, protein visualization, study of protein interactions, controlled...Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled Expression...plasmids that contain fluorescent protein (FP) tags so you can express your protein of interest fused to... Additional Addgene Protein Visualization Resources Visit our Fluorescent Protein Guide to find an extensive...

...Plasmid Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular Localization...marker protein is fused to EGFP, you may want to fuse your protein to a red fluorescent protein like mCherry... different fluorescent protein markers? Check out the Michael Davidson Fluorescent Protein Collection ... Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Content last reviewed...pattern, you should fuse your protein of interest with a fluorescent protein that has a different emission...overlap between your fusion protein and the marker fusion protein using fluorescence microscopy, you can assess...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...

... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones Blue/UV...Return to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue...Botman-Teusink Yeast Fluorescent Protein Collection Plasmid Kits Hamdoun Lab Fluorescent Protein Fusion Construct...Blog: Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your Fluorescent Proteins for Multi-Color...popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins, along ...plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest. Plasmid...experiment. Tables are organized by color of the fluorescent protein. The content for this page was originally...

...encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...hSyn1-eYFP An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter Viviana Gradinaru...

...expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression in ...EcoFlex MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and... : 285 vectors for expression, markers, fusion proteins, etc - Lindquist Lab Synthetic Biology BIOFAB ...

...collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors...pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more. Addgene...Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral.... Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs...RESOLUTE Plasmid Collection for Solute Carrier Proteins (SLCs) Structural Genomics Consortium Zinc Finger...resources group plasmids together based on type or application. Browse our guides to find the plasmids and science...

...Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal...Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny ...Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neurons...Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong ...Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium spiny...Alias: CN2237) Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny...

...then purified from the supernatant using Protein A or Protein G affinity columns. Formulation Buffer After...measured and the ratio of the antibody protein content to the total protein content of the sample is determined...and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared to...secondary antibody. Protein expression is compared to an untransfected control and the protein size is confirmed...by Addgene were developed by the Institute for Protein Innovation (IPI). For these antibodies, IPI will...these antibodies, please visit the Institute for Protein Innovation Antibody Collection webpage. Antibody...concentration and stained with Coomassie blue. Protein band intensities are measured using ImageJ software...

... and CRISPR tools Fluorescent Proteins from the Michael Davidson collection Protein Labeling from New ...multigene constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for detecting...of cell types. Protein Purification Optimization Kit Using SUMO Protease Elution Protein Characterization... Structural Genomics Consortium Solute Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging...Bahl A validation set of 96 unique, single-domain proteins that are structurally characterized (PDB-deposited...antibodies are produced in-house and undergo application-specific validation and quality control by Addgene... Recombinant Antibody Reactivity Recommended Applications PI Don't See What You're Looking For? Please...

... DNA repair protein, and is suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag...complementary protein function or localization. ACP and MCP tags ACP- and MCP-tags are small protein tags based... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ... Users may still generate their own fluorescent or non-fluorescent CoA substrates by purchasing coenzyme...internal or surface proteins Single constructs are compatible with multiple applications (different fluorophores...ACP or MCP tagged plasmids for visualization of proteins inside cells. Depositor...Information New England Biolabs offers non-toxic protein labeling systems based on SNAP-, CLIP-, ACP-, and...

...amino acids) fragments for protein-protein interaction assays. Each target protein is fused to either LgBiT...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence...Luciferase Complementation assays can report on protein-protein interactions, drug-induced changes, and more... about BRET on our blog . Nano-Lanterns: Fluorescent proteins fused to Renilla luciferase ( Nano-lanterns...secreted version of the protein is available, making it ideal for a variety of applications. To learn more and...Both firefly and Renilla luciferase have short protein half-lives, making them useful as transcriptional...transcriptional reporters, but are less-than-ideal protein tags due to their large size (61 kDa and 36 kDa, respectively...

...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...primarily show a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect...

...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types... to DNA. Others inhibit the recruitment of Cas proteins to the CRISPR-Cas complex. Recently, researchers... can include mutations seen in human patients, protein tags, or loxP/FRT sites, among others. Homology-directed...used to purify a genomic locus and its associated proteins or RNAs through ChIP (enChIP) or biotin-streptavidin...

...Assays monitoring impedance, fluorescence, mass/charge properties, protein thermal stabilization, and changes...to boost the research focused on Solute carrier proteins (SLCs). Browse the SLC plasmid collection. Plasmid...Pooled Libraries Feedback Resources Solute carrier proteins (SLCs) are the largest family of transporters ...contribute to assess ligand action/engagement. Protein production is used for assays and to generate antibody... libraries target the solute carrier family of proteins. Library ID PI Description Human SLC Activation... CRISPR activation library targeting human SLC proteins. Human SLC Knockout Library 132552 Superti-Furga...Superti-Furga CRISPR knockout library targeting human SLC proteins. Mouse SLC Knockout Library 141436 Superti-Furga...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...specificity and applicability of base editing through protein engineering and protein delivery. Nature...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ..., or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...

...Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent protein into your expression vector allows you to...drug-inducible approach may also be used. Split proteins: The recombinase is split into inactive N- and...fragments in the same cell reconstitutes a functional protein, allowing recombination of the DNA between target...Drosophila Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing... Plasmid Collections AAV Enhancer Collection Fluorescent Biosensors Chemogenetics Plasmid Collection Optogenetics...a genomic attP site. So far, their practical application has been limited by low integration efficiency...

... CRISPR Protein Tagging CRISPR Plasmids - Protein Tagging Browse CRISPR Plasmids...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and ...134.7 KB Mendenhall and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid...on endogenous genes for the isolation of native protein complexes. This vector serves as a backbone to ...2A-PuroR cassette to the C-terminus of endogenous proteins. The PITCh system depends on MMEJ (microhomology-mediated...

...vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully). This next...require cloning of left and right TALEN monomer proteins into opposite vectors. 37276 pCS2TAL3-RR 48636...deletion from the N terminus of the wild-type TALE protein (previously shown to preserve the DNA-binding ability...plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize subcellular positioning...length human LSD1 at the carboxy-terminus of the protein. 49043 EMM67 49044 EMM71T Golden Gate TALEN 2.0...Golden Gate TALEN 2.0 48705 pTAL7a Boris Greber Application of TALEN technology in hPSCs pTAL7a and pTAL7b...