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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12 results for: Gene Tools Blue

Showing: 1 - 12 of 12 results

  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Structure Plasmids Fast-FT 403(blue), 583(red) 466(blue), 606(red) 14.9(blue), 6.8(red) 2.8/4.1 7.1 hr (red...Bacterial Expression Medium-FT 401(blue), 579(red) 464(blue), 600(red) 18.4(blue), 5.8(red) 2.7/4.7 3.9 hr (red...Bacterial Expression Slow-FT 402(blue), 583(red) 465(blue), 604(red) 11.7(blue), 4.2(red) 2.6/4.6 28 hr (red...? Background Fluorescent proteins are genetically encoded tools that are used extensively by life scientists...you to create fusion proteins with your gene of interest. Special...protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since then scientists have engineered...tags for you to create fusion proteins with your gene of interest. These are organized By Color of the...

  2. Optogenetics Plasmid Collection

    Type
    Collection
    ...Find optogenetics plasmids such as microbial opsin and optical switch tools to measure and manipulate...first microbial opsins to be adopted as an optogenetic tool is the widely used Channelrhodopsin-2 (ChR2...using these opsins as a tool to control neuronal activity, scientists generally attach a fluorescent protein... AAV preparations of many optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter... is a membrane channel that opens in response to blue light and allows cations to flow into the cell. ...transcription", or other terms. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging...Collections Optogenetics Plasmids Optogenetics Plasmid Collection You may also like... Optogenetics Guide AAV...

  3. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization ... PGK1 promoters and the KanMX marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional ... a new window) Lee S, Lim WA, Thorn KS. Improved blue, green, and red fluorescent protein tagging vectors... Vickers CE, Bydder SF, Zhou Y, Nielsen LK. Dual gene expression cassette vectors with antibiotic selection...Yeast Toolkit (MYT) Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other ...

  4. Bacterial Expression Systems

    Type
    Collection
    ...knocking out genes or introducing specific mutations can reveal important insights into gene function. These...expression of a gene of interest in E. coli . The Marionette Sensor Collection (Addgene #1000000137) allows...of up to twelve genes using small-molecule inducers in the same E. coli strain. Addgene Blog Inducible ...bacterial gene biology or engineering metabolic pathways to synthesize a target metabolite, genetic tools ...visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome engineering...transcriptional regulatory elements that will turn on gene expression upon protein–protein interaction. The...always advantageous to constitutively express your gene of interest at high levels. Perhaps high expression...

  5. TALEN Plasmids and Kits

    Type
    Collection
    ... order to generate TALORs (TAL Orthongal Repressors) that can be used to custom repress gene expression... successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent...Zinc Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription activator-like... Golden Gate TALEN kit, (ii) a lacZ fragment for blue/white-screening in E.coli , (iii) CAG promoter and...editing was also shown to alter regulation of nearby gene expression. The vectors listed below have the EF1α...kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids. Genome... EMM71T Golden Gate TALEN 2.0 49401 pBlue-TAL Michal Zurovec pBlue-TAL was designed for use with the Voytas...

  6. Luciferase Plasmid Collection

    Type
    Collection
    .... ID Plasmid Description Gene/Insert PI Return to Top Other Highlighted Tools Modified/Novel Luciferases...Explore Addgene's collection of luciferase plasmids for gene expression assays and bioluminescent reporters...to photobleaching have made luciferase a popular tool for scientists. Regulatory elements such as promoters...luciferase-containing plasmid and the effects on gene expression can be measured by the luminescence output...into which you can clone your regulatory element or gene of interest into to create a luciferase reporter...Firefly, Renilla Creating ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of...enhancer regions. Mutations within the luciferase gene allow for increased copy number in E. coli and expression...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Gadella Calcium Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca(2+) indicator...García-Sancho Calcium Genetically encoded calcium indicators for optical imaging (GECOs), including blue, green, red...cyclic GMP) FRET-based cGMP biosensor SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation...NTS, UCN, SST, and CRF A tool kit of highly selective and sensitive genetically encoded neuropeptide sensors...fluorescent biosensors to measure biomolecules or genes via FRET or other assays. Plasmid...specific biomolecules, the activity of specific genes and cellular processes, or other factors like environmental...mitochondria-targeted CEPIA calcium sensors (green, red or blue/green) Imaging intraorganellar Ca(2+) at subcellular...

  8. Antibody Guide

    Type
    Collection
    ...Selection Validation Affinity reagents are molecular tools used in the lab to specifically and tightly bind...Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into plasmids. Plasmids... contain highly variable regions, shown in light blue and light orange, that bind to a specific antigen...called the constant region, and is shown in dark blue and dark orange. The constant region is a stable... tubulin (sometimes referred to as housekeeping genes). This allows for relative comparison of expression...complexes for downstream analysis. They are powerful tools for understanding protein or protein interactions...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...

  9. Optogenetics AAV Preps

    Type
    Collection
    ...Service AAV Optogenetics Viral Vector Packaging Service: Optogenetics AAV Optogenetic tools can be used...fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength fluorescent protein Activity Cre dependent... potentials in neurons. See our Optogenetics Plasmids and Optogenetics Guide page for more information...ready-to-use AAV preparations of optogenetics plasmids available in Addgene's collection. Opsin Excitatory...select plasmids in the repository. Browse our optogenetics AAV collection, including channelrhodopsins,...control cell activity in living tissue with light. Optogenetics is typically used in neuroscience to control...consider using fewer filtering options. Browse all Optogenetics AAV *AAVrg = retrograde serotype, produced with...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... are excellent tools for further understanding the function of your favorite gene. For example, fusing... vector for gene expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression ...destination vector for gene expression Lentiviral backbones made by Addgene Addgene has an extensive collection...host cell and are designed for a user to insert a gene of interest. They may contain tags or fusion proteins...These vectors allow you to test the function of your gene of interest in a controlled environment under various...we will assume that you want to express a gene; however, we have included a function section for if you...If you want to drive expression of your favorite gene, you will need a plasmid with a promoter that...

  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires that you have ...are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into...capable of simultaneously repressing multiple target genes in Escherichia coli. Koffas Do you have suggestions...BsaI none S. pyogenes Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol...none S. pyogenes Qi pDD162 (Peft-3::Cas9 + Empty sgRNA) 47549 C. elegans yes, cut S. pyogenes Goldstein...elegans BsaI none S. pyogenes Joung pCFD3-dU6:3gRNA 49410 Drosophila BbsI none S. pyogenes Virmilion Bullock..., cut S. pyogenes Puro Liu pCFD4-U6:1_U6:3tandemgRNAs 49411 Drosophila BbsI none S. pyogenes Virmilion...

  12. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange)....deposited with Addgene for distribution to the research community. Protocols for the generation R-mAbs plasmids...researchers will be able to easily and inexpensively generate R-mAbs by transiently transfecting the plasmids...produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species Host Species Isotype...musculus (mouse)/R. norvegicus (rat) rat IgG2a Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More...produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target Species Host...
Showing: 1 - 12 of 12 results