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We narrowed to 17 results for: N-EGFP

Showing: 1 - 17 of 17 results
  1. The Pleiades Promoter Project

    Type
    Collection
    ... cre N/A N/A pEMS1301 cre/EGFP/NLS N/A N/A pEMS1308 EGFP/cre N/A N/A pEMS1302 EGFP/cre/NLS N/A N/A pEMS1306...pEMS1306 EGFP/NLS N/A N/A pEMS1307 EGFP/NLS N/A N/A pEMS1313 intron-lacZ/NLS Positive Control CAG promoter...pEMS1293 cre N/A CAG promoter pEMS1164 EGFP/cre N/A CAG promoter pEMS1114 EGFP/cre/NLS N/A CAG promoter...pEMS1157 EGFP/NLS N/A CAG promoter pEMS1277 EGFP/NLS N/A CAG promoter pEMS1294 intron-lacZ/NLS N/A CAG promoter...pEMS1153 EGFP/NLS Ple37 CRH pEMS1154 EGFP/NLS Ple38 CRH pEMS1155 EGFP/NLS Ple39 CRH pEMS1156 EGFP/NLS Ple44...pEMS1113 EGFP/cre/NLS Ple51 DBH pEMS1362 EGFP/NLS Ple52 DCX pEMS1198 EGFP/NLS Ple53 DCX pEMS1199 EGFP/NLS ...pEMS1167 EGFP/NLS Ple63 DRD1 pEMS1168 EGFP/NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS ...
  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Primary cilia Htr6 EGFP Kirk Mykytyn 104358 pEGFP-N-Drd1 Primary cilia (Neurons) Drd1 EGFP Kirk Mykytyn *Fusions...Rab8 EGFP Maxence Nachury 35623 pEGFPN3-Sstr3 Primary cilia Sstr3 EGFP Kirk Mykytyn 35624 pEGFPN3-5ht6...Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin filaments F-tractin EGFP Dyche Mullins 41147 pEGFP Centrin2 Centrioles...Cortactin EGFP Anna Huttenlocher 32907 pEGFP-rNFM Intermediate filaments (neuronal) Nefm EGFP Anthony Brown...Centrin-2 EGFP Erich Nigg 41151 pEGFP Cep170 C-term Centrioles (dependent on cell cycle) Cep170 EGFP Erich...Palmitoylation EGFP Connie Cepko 14757 pCAG-mGFP Membranes Palmitoylation sequence from GAP43 EGFP Connie Cepko... mCherry Gia Voeltz 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru 122871 GFP-hCCDC11 ...
  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging ...Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin...that adds an N-terminal myristoylation signal GST Protein purification pEBG - N-terminal GST... N-terminal His6-GST-TEV for bacterial expression pDest-565 - N-terminal ...Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible empty backbones...proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression pLV-mCherry...tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV cleavage Transient expression...
  4. CRISPR Plasmids - Tagging

    Type
    Collection
    ...provided detailed protocols for N- or C-terminal tagging in Drosophila cells. N terminal tagging in Drosophila...PITCh system plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous...the donor vector. The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences...first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin... gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus Jorgensen... the donor plasmids containing homology arms and EGFP are available at Addgene. Do you have suggestions...Doyon's lab deposited a plasmid which introduces an N- or C- terminal affinity tag (3xFLAG-2xSTREP) on endogenous...
  5. Recombinases AAV Preps

    Type
    Collection
    ...tdTomato 9 William Pu 203842 pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE EF1a EGFP 8 Ivo Spiegel 105550 pAAV.GFAP.Cre.WPRE.hGH...9, rh10 James M. Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632...Syn EBFP 5 Hongkui Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB James... Esteban Engel , Alexander Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1 Connie Cepko 69571 pAAV-EF1a-C-CreintG...3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3780) minBG N/A 2 Allen Institute for Brain Science , Jonathan Ting...
  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1 SOD1 H1 ALS Patrick Aebischer 10881 pCCL cPPT PGK EGFP WPRE LTR H1...14121 pcDNA3-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP...Dantuma 23971 VCP(wt)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23972 VCP(R155H)-EGFP VCP His, GFP, HA CMV...Dantuma 23973 VCP(A232E)-EGFP VCP His, GFP, HA CMV ALS Nico Dantuma 23974 VCP(DK0)-EGFP VCP His, GFP, HA CMV...Merrifield 28195 tdp43-EGFP construct2 TARDBP GFP CMV ALS Zuoshang Xu 28196 tdp43-EGFP construct3 TARDBP GFP...Zuoshang Xu 28197 tdp43-EGFP construct4 TARDBP GFP CMV ALS Zuoshang Xu 28198 tdp43-EGFP construct5 TARDBP GFP...Zuoshang Xu 28199 tdp43-EGFP construct6 TARDBP GFP CMV ALS Zuoshang Xu 28200 tdp43-EGFP construct7 TARDBP GFP...
  7. Zhang Lab CRISPR Page

    Type
    Collection
    ... recombinase-2A-EGFP-KASH 60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone...recombinase-2A-EGFP-KASH and an sgRNA. #60231 - AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A ...SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro...) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase) and single guide RNA 62987 : PX462...20bp). #60230 - AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP...contains two expression cassettes, Cre recombinase-2A-EGFP-KASH and an sgRNA backbone for cloning new targeted...
  8. Lentivirus Plasmids

    Type
    Collection
    ...more variants. Tyler Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion with puromycin resistance. Can ...Stephen Elledge 21373 pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid...119816 pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase 3rd Expression of EGFP-Firefly luciferase fusion protein...14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1 selection. ...expression. David Sabatini 14883 FUGW 3rd hUbC-driven EGFP. Can be used for cDNA expression. David Baltimore...3rd Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression...silencing. Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression...
  9. Tetracycline Inducible Expression

    Type
    Collection
    ...pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression of transgene of interest with EGFP (On or...Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP...Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS Jason Sheltzer 11651 pLVUT-tTR-KRAB... Off) None TRE, miniCMV Maria Lung 16542 pBI-MCS-EGFP Bidirectional promoter (Pbi) for Tet-responsive ...expression (On or Off) of both your gene of interest and EGFP. Pbi contains a TRE between two minimal CMV promoters...Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection rtTA-Advanced Mikhail ...TLCV2 Lentiviral vector for tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 . Adam Karpf...
  10. Fluorescent Proteins: FRET

    Type
    Collection
    ...-sREACh-N3 EGFP ShadowY** 488 0.6 531** 136,000 0.01 6.1 4.5 mEGFP-N1 , CMV-ShadowY , EGFP-ShadowY λ Dex...mTFP1-N1 , mCitrine-pBAD EGFP mCherry 488 0.60 610 72,000 0.22 5.3 1.9 mEGFP-N1 , mCherry2-N1 , pcDNA3.1...sReach-mTurquoise2 EGFP sREACh** (super-REACh, aka Nữ) 488 0.60 531** 115,000 0.07 6.0 4.1 mEGFP-N1 , sREACh-...pcDNA3.1 CMV mCherry-eGFP BGH Clover mRuby2 505 0.76 600 113,000 0.38 6.3 4.5 pcDNA3-Clover , pcDNA3-mRuby2...opens in a new window) Algar, W. R., Hildebrandt, N., Vogel, S. S., & Medintz, I. L. (2019). FRET as a...
  11. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Structure 87420 PXN-EGFP AICSDP-1 EGFP Paxillin Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin..., Horwitz, R., Rafelski, S. M., & Gunawardane, R. N. (2017). Systematic gene tagging using CRISPR/Cas9...87422 LMNB1-mEGFP AICSDP-10 mEGFP Lamin B1 Nuclear envelope 87423 TOMM20-mEGFP AICSDP-8 mEGFP Tom20 Mitochondria...Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin... SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic reticulum 87427 FBL-mEGFP AICSDP-13 mEGFP Fibrillarin...101782 LAMP1-mEGFP AICSDP-19 mEGFP LAMP-1 Lysosome 101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related...101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP Nucleophosmin...
  12. CRISPR References and Information

    Type
    Collection
    ...plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for human and...pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your input sequence...and can work for S. pyogenes , S. thermophilus , or N. meningitidis Cas9 PAMs. Currently supports: mouse...
  13. Luciferase Plasmid Collection

    Type
    Collection
    ... with a fluorophore as the acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc ( OgNLuc... 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko...87078 pLenti6.2-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral...UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash... 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko... John Atkins 174050 pGWB-nLUC Firefly Creation of N-terminal Firefly luciferase fragment for split-luciferase...NanoLuc® CMV Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc...
  14. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Bacterial Expression EGFP 488 507 34 6 25 min Prone to dimerization pcDNA3-EGFP - Mammalian Expression...Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish...Sea urchin pCS2+8NeGFP - Zebrafish/Xenopus/Worm/Sea urchin pKT0128 - Yeast Expression EGFP-pBAD - Bacterial...Expression cfSGFP2 493 517 34 5.4 Monomer (A206K) cfSGFP2-N - Mammalian Expression (cysteine-free SGFP2) ZsGreen...w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo - Xenopus Expression pET51b-His-TEV-HaloTag7 ...w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression pSNAP-tag (T7) Vector -...Bacterial Expression mEGFP 488 507 34 6 Monomer (A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian...
  15. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...pAdx-CMV-iCre-p2A-copGFP 73350 Expresses iCre and EGFP from the CMV promoter pAdx-CMV-iCre-P2A-tdTomato...Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256...
  16. Genetic Code Expansion

    Type
    Collection
    ...Phosphoserine Bacterial TAG Jesse Rinehart 191118 pCS2-EcYRS-EGFP* TyrRS E. coli O-methyltyrosine (O-Me-Tyr) Mammalian...Mammalian TAG Han Xiao 191119 pCS2-MfnG-EcYRS-EGFP* TyrRS E. coli O-methyltyrosine (O-Me-Tyr) Mammalian TAG...TAG Abhishek Chatterjee 228785 pAcBac3-EcLeu TAG-EGFP** LeuRS E. coli Cy5-azide (Cy5Az) Mammalian TAG ...TAG Abhishek Chatterjee 228786 pAcBac3-EcTrp-TGA-EGFP** TrpRS E. coli 5-hydroxytryptophan (5HTP) Mammalian... Abhishek Chatterjee 228787 pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA OMeYRS E. coli O-methyltyrosine... Abhishek Chatterjee 228788 pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA pAAFRS E. coli p-aminophenylalanine...Hang 126035 pEVOL-ABK PylRS M. barkeri 3’-azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio...
  17. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...IgG2a 182104 Anti-LRRK2/Dardarin, N-terminus [N138/6R] LRRK2/Dardarin, N-terminus Human Mouse IgG2a 182105... 135224 GC52 pEGFPN1 anti-Gephyrin nanobody GC52 Gephyrin Mouse Llama 135225 AC50 pEGFPN1 anti-AMIGO-1...Llama 135226 AC27 pEGFPN1 anti-AMIGO-1 nanobody AC27 AMIGO-1 Mouse Llama 135227 IC65 pEGFPN1 anti-IRSp53 nanobody... nanobody IC65 IRSp53 Mouse Llama 135228 SS80 pEGFPN1 anti-SAPAP2 nanobody SS80 SAPAP2 Mouse Llama 145818...
Showing: 1 - 17 of 17 results