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We narrowed to 93 results for: expression vector

Showing: 1 - 20 of 93 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-...versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins ...Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP...Baculovirus expression vector with N-terminal GST pGCS-N5(GST) - GST tagged Gateway destination vector for mammalian...

  2. Kazuhiro Oka Lentiviral Vectors

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    ...Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral...Transfer Vectors Adenoviral Vectors Kazuhiro Oka and the Baylor College of Medicine Gene Vector Core (Link...promoter when Cre is expressed by another vector pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 promoter...promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato 72261 Expresses tdTomato from the ... promoter when FLP is expressed by another vector pCDH-EF1-FLPe 72262 Expresses FLPe from the EF-1 promoter...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral...and Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and ...

  3. Tetracycline Inducible Expression

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    ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter ...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression You may also like... Blog...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...Experimental Considerations Expression System If your gene of interest should only be expressed occasionally, use...respectively. You should also choose between: Dual vector system: express the transactivator and the tet-controlled...

  4. Brain Initiative Collection

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    ...-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD expression. Bryan Roth 75033-AAV1 pAAV ...axon-GCaMP6s-P2A-mKate2 For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2 Lin Tian 112005...axon-GCaMP6s-P2A-mRuby3 For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3 Lin Tian 112005...axon-GCaMP6s-P2A-mRuby3 For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3 Lin Tian 112006...cre-dependent enriched expression of GCaMP6s in axons Lin Tian 113049-AAV9 pAAV-hSyn-GRAB_DA1m express the genetically-encoded...Chemogenetics expression plasmid Scott Sternson 123308-AAV9 pAAV-hSyn-GRAB_NE1m Expresses the genetically-encoded...135630-AAV1 pAAV-S5E2-dTom-nlsdTom AAV vector to drive the expression of dTomato in PV cortical interneurons...

  5. Zinc Finger Consortium: Nuclease Expression Vectors

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    ...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger...transferred in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...in-frame to these vectors. Plasmid...heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric FokI domain. Miller et...

  6. Cre-Lox and Other Site-Specific Recombinases

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    ... plasmids expressing Cre recombinase and filter the results by expression system and vector type. Find... GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout/Knock-In... plasmids expressing Flp recombinase and filter the results by expression system and vector type. Find... plasmids expressing Dre recombinase and filter the results by expression system and vector type. Find...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with loxP...

  7. TALEN Plasmids and Kits

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    ...UTRs are included in the vector to enhance expression of the message. Both vectors utilize homodimeric FokI...contains modified pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based ...Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing...much higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs...placing TALORs built into this vector under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter...promoter, giving constitutive expression of TALORs built into this vector. 36034 pTAL6-BB Golden Gate TALEN...1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter N- and C-terminal tal...

  8. TALEN Expression Vectors

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    ...TALengineering Reagents TALEN Expression Vectors TALEN Expression Vectors for REAL, REAL-Fast and FLASH...same). All expression vectors listed below have the CMV promoter for mammalian cell expression and a T7 ...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH...in vitro transcription of RNA. TALENs expressed from these vectors also have a FLAG epitope tag and nuclear...localization signal at their amino-terminus. The expression vectors listed below can be purchased together with...plasmids CRISPR plasmids This page lists plasmid vectors into which DNA fragments encoding TAL effector ...REAL-Fast or FLASH methods) can be cloned for expression as TALENs. Additional details about the REAL,...

  9. Zhang Lab CRISPR Page

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    ...2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator...lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with ...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for cancer modeling Using Cas9 mice, Platt...

  10. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...pEiLOV-N1 - Mammalian Expression pGEX iLOV - Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... - Mammalian Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1... Mammalian Expression mTopaz-C1 - Mammalian Expression mTopaz-pBAD - Bacterial Expression mVenus 515 528...

  11. Plant Plasmids and Resources

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    ...creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene silencing...maize and Arabidopsis) and vectors for easy assembly of one or more gRNA expression cassettes - Qi-Jun Chen... GreenGate Cloning System to create plant expression vectors containing several cassettes and generate...microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system based on... - Tools for gene and protein expression and mapping of gene expression in plants. Figshare - Plant Illustrations... that drive general and species-specific gene expression (see the column ‘promoter’ in the tables for ...CRISPR Resources for a full selection of plasmids expressing different functional variants of Cas9 and empty...

  12. Luciferase Plasmid Collection

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    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ...Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression Renilla...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia...Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression Stephen Tapscott 83281 ...Mammalian expression of firefly luciferase Lee Helman 131860 pZmUBQ:LUC Firefly ZmUBQ Plant expression of firefly...Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc Renilla CAG Vector for packaging...

  13. SARS-CoV-2 Pseudotyped Virus

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    ...reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry...mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized...Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-...can then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in ...Resources page for more information. Plasmids Expressing Spike and Spike Variants Full-length wildtype...

  14. Antibody Plasmid Collection

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    ... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection of recombinant.... Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem...problem of in-frame amber codons in scFvs A new expression vector facilitating production and functional analysis... trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large...cloning antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast, plants...length recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are ...

  15. AAV Molecular Tools

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    ... Viral Vector Packaging Service AAV Molecular Tools Viral Vector Packaging Service: Molecular...with tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118...99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru ...pAAV-ihSyn1-tTA Inducible Synapsin promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive...positive feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent...inducible Synapsin promoter (ihSyn) Cre-dependent expression of the tet-off transactivator (tTA) with a positive...positive feedback loop for amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and...

  16. Plan Your Experiment

    Type
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    ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes a bit more prep work...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and...

  17. Adeno-associated virus (AAV) Plasmids

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    ...Blog: Plasmids 101-Viral Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids...plasmid, expressing Rep2 and Cap1 James M. Wilson 104963 pAAV2/2 AAV2 AAV packaging plasmid, expressing Rep2...plasmid, expressing Rep2 and Cap5 Melina Fan 240485 pAAV2/6 v1 AAV6 AAV packaging plasmid, expressing Rep2...plasmid, expressing Rep2 and Cap7 James M. Wilson 112864 pAAV2/8 AAV8 AAV packaging plasmid, expressing Rep2...vivo . While AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-...not integrate into the host genome, transgene expression can be long-lived. The utility of AAV is currently...Pseudotyping Want to save time? Check out our Viral Vector Packaging Service and learn about our options for...

  18. CRISPR Plasmids - Empty gRNA Vectors

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    ... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...size and allow for expression of more sgRNAs in a single vector. sgRNAs are expressed as polycistronic ...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base...by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI...BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none S. pyogenes Huang...

  19. Plasmids for Stem Cell Research

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    ...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human...polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative...Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 ...iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and...transdifferentiation utilize plasmids to increase expression of key factors. The tables below will help you...

  20. Brain Armamentarium

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    ...-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic interneurons. For...version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons...-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons..._3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium spiny neurons...P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate...3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium...3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) Expression of jGCaMP8s in striatal direct pathway medium...
Showing: 1 - 20 of 93 results