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We narrowed to 80 results for: expression vector

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Showing: 1 - 20 of 80 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...gene expression vector URA3 Yeast pAG306GAL-ccdB - Yeast Gateway expression vector (see...Inducible Expression page Bacteria Lac, T7, araBAD, trp mTagBFP2-pBAD - Protein expression vector with C-...versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins ...Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP...Baculovirus expression vector with N-terminal GST pGCS-N5(GST) - GST tagged Gateway destination vector for mammalian...

  2. Kazuhiro Oka Lentiviral Vectors

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    Collection
    ...Collections Gene Vector Core Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral... transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...promoter when Cre is expressed by another vector pCDH-EF1-DIO-tdTomato 72254 Expresses tdTomato under EF-1 ...promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato 72261 Expresses tdTomato from the ... promoter when FLP is expressed by another vector pCDH-EF1-FLPe 72262 Expresses FLPe from the EF-1 promoter...lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral Vector Guide Find more plasmids...Cre is expressed by another vector pCDH-CB-iCre-P2A-tdTomato-T2A-Puro 72255 Cre is coexpressed with tdTomato...

  3. Tetracycline Inducible Expression

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    Collection
    ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter ...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Tetracycline Off Tetracycline...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...Experimental Considerations Expression System If your gene of interest should only be expressed occasionally, use...respectively. You should also choose between: Dual vector system: express the transactivator and the tet-controlled...

  4. Brain Initiative Collection

    Type
    Collection
    ...-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD expression. Bryan Roth 75033-AAV1 pAAV ...axon-GCaMP6s-P2A-mKate2 For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2 Lin Tian 112005...axon-GCaMP6s-P2A-mRuby3 For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3 Lin Tian 112005...axon-GCaMP6s-P2A-mRuby3 For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3 Lin Tian 112006...cre-dependent enriched expression of GCaMP6s in axons Lin Tian 113049-AAV9 pAAV-hSyn-GRAB_DA1m express the genetically-encoded...Chemogenetics expression plasmid Scott Sternson 123308-AAV9 pAAV-hSyn-GRAB_NE1m Expresses the genetically-encoded...135630-AAV1 pAAV-S5E2-dTom-nlsdTom AAV vector to drive the expression of dTomato in PV cortical interneurons...

  5. Adeno-associated virus (AAV) Plasmids

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    Collection
    ...Adeno-Associated Viral (AAV) Vector Guide All Viral Plasmids Viral Vectors 101 eBook Viral Vectors 101 Youtube Videos... AAV vector production suitable for a variety of serotypes and applications. Read our AAV vector guide...production, and viral safety. AAV Vector Guide Want to save time? Our Viral Vector Packaging Service has hundreds...plasmids) used by Addgene's Viral Vector Packaging Service to produce AAV vectors for all available serotypes...plasmid, expressing Rep2 and Cap1 James M. Wilson 232199 pAAV2/2 AAV2 AAV packaging plasmid, expressing Rep2...plasmid, expressing Rep2 and Cap7 James M. Wilson 112864 pAAV2/8 AAV8 AAV packaging plasmid, expressing Rep2...AAV Helper Plasmids Adeno-associated viral (AAV) vectors offer precise delivery of genetic material across...

  6. Zinc Finger Consortium: Nuclease Expression Vectors

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    Collection
    ...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger...transferred in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...in-frame to these vectors. Plasmid...heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric FokI domain. Miller et...

  7. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... plasmids expressing Cre recombinase and filter the results by expression system and vector type. Find... GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout/Knock-In... plasmids expressing Flp recombinase and filter the results by expression system and vector type. Find... plasmids expressing Dre recombinase and filter the results by expression system and vector type. Find...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with loxP...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...UTRs are included in the vector to enhance expression of the message. Both vectors utilize homodimeric FokI...contains modified pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based ...Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing...much higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs...placing TALORs built into this vector under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter...promoter, giving constitutive expression of TALORs built into this vector. 36034 pTAL6-BB Golden Gate TALEN...1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter N- and C-terminal tal...

  9. Plant Plasmids and Resources

    Type
    Collection
    ...species-specific gene expression. Cloning and Synthetic Biology Expression Vectors The table below highlights...collections are designed for creating plant expression vectors. ID Kit Description Cloning Type PI 1000000036...MultiGreen Kit Modular assembly of multi-gene expression vectors. Golden Gate (GreenGate) Wayne Parrott Additional...Resources Improved Gateway binary vectors — High-performance vectors for creation of fusion constructs...maize and Arabidopsis) and vectors for easy assembly of one or more gRNA expression cassettes. A CRISPR/Cas...multigene expression, gene silencing, and silencing rescue in plants. JoinTRV , an improved vector system... — Tools for gene and protein expression and mapping of gene expression in plants. Grassius (Link opens...

  10. Zhang Lab CRISPR Page

    Type
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    ...2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator...lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding...sgRNA to a constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with ...61408 : Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling...toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for cancer modeling Using Cas9 mice, Platt...

  11. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...MiCy-N1 - Mammalian Expression MiCy-C1 - Mammalian Expression MiCy-pBAD - Bacterial Expression Return to top... - Mammalian Expression sfGFP-C1 - Mammalian Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... - Mammalian Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...a reference level of expression. Browse or search for popular plasmids expressing luciferase. We also ...Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression Renilla...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia...Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression Stephen Tapscott 83281 ...Mammalian expression of firefly luciferase Lee Helman 131860 pZmUBQ:LUC Firefly ZmUBQ Plant expression of firefly...Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc Renilla CAG Vector for packaging...

  13. Antibody Plasmid Collection

    Type
    Collection
    ... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection of recombinant.... Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem...problem of in-frame amber codons in scFvs A new expression vector facilitating production and functional analysis... trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large...cloning antibody components into plasmids and expressing these in bacteria, mammalian cells, yeast, plants...length recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are ...

  14. Retrovirus Plasmids

    Type
    Collection
    ...GFP MoMLV U6-driven plasmid for shRNA expression with GFP expression. See plasmid 8452 for puromycin resistance...Luciferase MSCV Plasmid for transgene expression with luciferase expression. Scott Lowe 9044 pMIG MSCV Plasmid...Plasmid for transgene expression with GFP expression. See plasmid 12282 for the addition of WRE, a post-transcriptional...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for additional...pLncEXP CMV/MSV lncRNA expression plasmid. Lei Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase in ...pMD2.G VSV-G-expressing envelope plasmid. Didier Trono 35617 pCAG-Eco Ecotropic MLV expressing envelope plasmid... Resources Gamma-Retroviral Vector Guide All Viral Plasmids Viral Vectors 101 eBook Biosafety Resource...

  15. Plan Your Experiment

    Type
    Collection
    ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes a bit more prep work...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and...

  16. Lentivirus Plasmids

    Type
    Collection
    ...long-term transgene expression. Wild-type lentiviruses have been engineered into viral vectors for safe use ...Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR... (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See Ventura et al....cDNA expression. David Sabatini 14883 FUGW 3rd hUbC-driven EGFP. Can be used for cDNA expression. David... constitutive transgene expression. Contains intron that gives high expression. Didier Trono 12254 pWPI...plasmid for cDNA expression. Miguel Ramalho-Santos 24129 pULTRA 3rd Bicistronic expression of EGFP and the...pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression. See plasmid 21374 for dTomato...

  17. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...gRNA Expression Vectors gRNA and Cas gRNA Only Multiplex Resources CRISPR requires you to express both...gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...collection of empty gRNA vectors. Explore our most popular plasmids for expression of gRNA and Cas, gRNA ... CRISPR Empty gRNA Vectors CRISPR...tables below. Looking for non-targeting control vectors? Search our catalog for control gRNAs . All-in-...All-in-one Plasmids (gRNA and Cas) ID Plasmid Vector Type Promoter Selectable Marker PI Publication Return ...Return to top gRNA-only Plasmids ID Plasmid Vector Type Promoter Selectable Marker PI Publication Return to...

  18. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...reporter vector expressing firefly luciferase and ZsGreen. Alejandro Balazs 170674 pBOBI-FLuc Express firefly...mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase to simultaneously...Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin resistance... - EFS-EGFPd2PEST-2A-MCS-Hygro Lentiviral vector expressing destabilized EGFP. Neville Sanjana 164432 ...Nemazee 161030 Zhou-pLOV-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter in...Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-...can then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in ...

  19. Viral Vector Guides and Plasmids

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    ...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile tools because...non-dividing cells. These virusal vectors enable long-term stable gene expression and are permanently integrated...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ...immunogencity. These viral vectors can direct long-term transgene expression, but generally don't integrate... Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to...

  20. Plasmids for Stem Cell Research

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    Collection
    ...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human...polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative...Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, and Sox2 ...iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and...transdifferentiation utilize plasmids to increase expression of key factors. The tables below will help you...
Showing: 1 - 20 of 80 results