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We narrowed to 54 results for: guide rna expression plasmid

Showing: 1 - 20 of 54 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...with the guide RNA. For most applications, we recommend using Cas9 with the single guide RNA (sgRNA), which...SpCas9n, D10A nickase) + single guide RNA: These plasmids contain two expression cassettes, a human codon-optimized...SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9-2A-Puro and single guide RNA 48873 : PX460; SpCas9n...) and single guide RNA 48140 : PX461; SpCas9n-2A-EGFP (D10A nickase) and single guide RNA 62987 : PX462...FACS sorting SaCas9 + single guide RNA: These plasmids contain two expression cassettes, a human codon-optimized...D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes. To target...both the SaCas9 gene and its single guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual...

  2. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... the RNA polymerase, thus strongly decreasing the expression level. Mismatches between the guide RNA and...range of expression levels. This collection comprises 20 plasmids, each carrying two CRISPR guides. The first... can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with... CRISPR Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...arrays to modulate the expression levels of two targets independently. The RNA-programmed DNA-binding ...nucleotides. Together, these plasmids allow to explore the whole range of expression levels for these two reporters...

  3. CRISPR Guide

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    Collection
    ...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA/DNA labeling... and editing (B) with Cas13. Browse Plasmids: RNA Targeting , RNA Editing Degrading DNA with Cas3 Rather...Repurposing CRISPR as an RNA-Guided platform for Sequence-Specific control of gene expression. Cell . 152 (5),...components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short...conversions using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target...delivering multiple gRNAs using a single plasmid ensures that all gRNAs will be expressed in the same cell...

  4. Plan Your Experiment

    Type
    Collection
    ...Cas enzyme and guide RNA) for your experiment (Figure 1). You will decide how to express Cas9, the delivery...delivery system for Cas9 and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate...and point mutations using a prime editing guide RNA (pegRNA) Prime editor (Cas9 nickase fused to reverse...is to directly transfect expression plasmids encoding your Cas protein and gRNA, which you generally perform...off-target effects. RNA delivery The first of these methods is RNA delivery of Cas and gRNA. This method involves... Science Guides CRISPR Guide Plan Your... your experiment) and the expression time (transient or stable). For plasmid and viral-based approaches...

  5. Viral Vector Guides and Plasmids

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    Collection
    ...available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors...viral vector guides and plasmids available at Addgene and other related resources. Science...Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors Resources...stable gene expression and are permanently integrated into the host genome. AAV Vector Guide | AAV Plasmids...capacity. Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred...lines or clinical use. Adenoviral Vector Guide | Adenoviral Plasmids Adenoviral vectors have high packaging...viral genome, expression of the transgene, packaging capacity, and size. Vector Type Expression Genome Packaging...

  6. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor...mutations using a prime editing guide RNA (pegRNA). As with a typical gRNA, the pegRNA is designed with a spacer...nicking sgRNA), cloning enzyme, selectable marker, and whether the plasmid contains Cas9. ID Plasmid Expression...other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...Drosophila ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  7. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-...dual guide construct. Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571...containing the guide target sequence: Up to two guides can be expressed from the same plasmid by utilizing...utilizing one of the dual guide shuttle plasmids to insert a second guide within a U6-guide cassette. This process...159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow conversion..., 237 KB) Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence Derivative...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several modifications...

  8. Open Enzyme Collection

    Type
    Collection
    ... Bacterial Expression Vectors Synthetic Biology Plasmids MoClo Guide Drew Endy Lab Plasmids Jennifer Molloy... protocols for expression in the Open Bioeconomy Lab's crowd-sourced expression guides (Link opens in ...Return to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase 165516...enzymes into an expression vector of their choice and then transform into E. coli for expression and purification...Golden Gate assembly. Plasmids Explore the collection and order individual plasmids via the links below:...Reverse Transcriptases RNA Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4...165516 pOpen-SP6rpol SP6 RNA Polymerase 165576 pOpen-T3gene1 T3 RNA Polymerase Return to top Other Enzymes...

  9. Plant Plasmids and Resources

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    Collection
    ... Plasmid Collections Plant Plasmids Plant Plasmids and Resources...creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene silencing...full selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few...addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use ...includes plasmids containing promoters that drive general and species-specific gene expression (see the... - deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...highlights plasmids that can be used as empty backbones for your gene of interest for expression in plants...

  10. CRISPR Plasmids - Mammalian Expression

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    Collection
    ...mammalian expression systems. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors...Purify RNA Editing RNA Knockdown Visualize Resources The following CRISPR plasmids have been designed for ...ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top RNA Knockdown ID Plasmid Gene/Insert...CRISPR Plasmids: Mammalian Expression Activate Base Edit Cut Epigenetics Interfere Nick Prime Edit Purify...Edit ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Cut ID Plasmid Gene/...Epigenetics ID Plasmid Gene/Insert Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert... ID Plasmid Gene/Insert Promoter Selectable Marker Publication Return to top Prime Edit ID Plasmid Gene...

  11. CRISPR Plasmids - Tagging

    Type
    Collection
    ...and Myers Tagging Plasmids Protein Species Tag Donor Plasmid gRNA plasmid gRNA plasmid FLAG pFETCh_Donor... Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...reaction produces a single plasmid targeting vector that encodes both a guide RNA transcript and a repair...the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt...PX458 . Multiple gRNA plasmids may be used. The HDR (homology directed repair) donor plasmid with homology..., which expresses an untagged Cas9 AND a gRNA targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG...

  12. Retrovirus Plasmids

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    Collection
    ...Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Additional...MSCV Plasmid for transgene expression with luciferase expression. Scott Lowe 9044 pMIG MSCV Plasmid for... shRNA expression with GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX...pLncEXP CMV/MSV lncRNA expression plasmid. Lei Sun 60683 pLXIN-Luc MoMSV Stable expression of luciferase...Gamma-Retroviral Vector Guide Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene...for transgene expression with GFP expression. See plasmid 12282 for the addition of WRE, a post-transcriptional...Use with packaging plasmid. Bob Weinberg 12259 pMD2.G VSV-G-expressing envelope plasmid. Didier Trono 35617...

  13. Zebrafish Plasmid Collection

    Type
    Collection
    ...Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...biosensors, and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful...fusion expression in organisms or cells in which an exogenous mRNA can be injected and expressed. EXPRESs...for plasmids containing your zebrafish gene of interest? Search Addgene's collection for plasmids that...

  14. CRISPR History and Development for Genome Engineering

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    Collection
    ...Cas proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs). Through sequence...guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine...check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene...CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . 32(3):279-84. PMID: 24463574...Weissman JS, Qi LS. 2013. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell... JA, Charpentier E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity... M, DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science . ...

  15. CRISPR Plasmids and Resources

    Type
    Collection
    ...structure/gene expression. RNA Targeting RNA Targeting Type VI CRISPR systems target RNA, not DNA, across...select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together ...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications...variety of factors. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed for use in bacteria.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...

  16. CRISPR Plasmids - dCas9-FokI

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    Collection
    ...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...tables below for CRISPR-FokI plasmids. Plasmids are available for expression in mammalian systems and Drosophila...Drosophila. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  17. Lentiviral Prep Service

    Type
    Collection
    ...ways. Cas9 proteins can be guided to specific genomic sites by an RNA guide (gRNA) that harbors targeting...empty gRNA plasmids . For more information on CRISPR plasmids and function, see our CRISPR plasmids resource...are made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...biological processes. For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes... of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes Cas9 protein...Zhang Cas9 and Accessories for Activating Gene Expression Catalytically-dead Cas9 (dCas9) can be fused ...

  18. CRISPR Plasmids - Bacteria

    Type
    Collection
    ... bacteria. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ... Nick Prime Edit Purify RNA Targeting Resources The following CRISPR plasmids have been designed for use...Activate ID Plasmid Gene/Insert Promoter PI Publication Return to top Base Edit ID Plasmid Promoter PI... top Cut ID Plasmid Gene/Insert Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert...top Nick ID Plasmid Gene/Insert Promoter PI Publication Return to top Prime Edit ID Plasmid Gene/Insert...10.1016/j.bbrc.2013.08.013. Return to top RNA Targeting ID Plasmid Promoter PI Publication Return to top ...selection of CRISPR plasmids and resources. Find more CRISPR functions along with plasmids categorized by ...

  19. CRISPR Plasmids - Empty gRNA Vectors

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    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...constructing all-in-one expression vectors containing multiple guide RNA expression cassettes and a Cas9 ...for sgRNA expression. Chen pCFD4-U6:1_U6:3tandemgRNAs Drosophila Single plasmid for the expression of ...Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut...can be used to express multiple gRNAs from a single plasmid with or without expression of Cas9. Highlighted.... Can express Cas9 with up to 3 gRNAs. Frew pSQT1313 Mammalian Single plasmid for the expression of multipe...Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag...

  20. Fluorescent Protein Guide: Biosensors

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    Collection
    ... Optogenetics Plasmids Viral Service: AAV Biosensors Luciferase Plasmids Promega Plasmid Collection Explore... of S. cerevisiae MOSAIC Kit includes 35 plasmids expressing a variety of fluorescent sensors to monitor...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...S-adenosyl methionine Reporters of miRNAs Toehold sensors for various target RNAs Additional Resources Addgene...Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors...our catalog . Use the article links to find the plasmids associated with the article. We also offer ready-to-use...
Showing: 1 - 20 of 54 results