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We narrowed to 26 results for: lentiviral vector plasmid

Showing: 1 - 20 of 26 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral vector for EGFP fusion...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF ...dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...

  2. Plasmids for Stem Cell Research

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    Collection
    ... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells... table based on plasmid name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication... 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector for the expression of human.... 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human...-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the expression...151-9. Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression...

  3. CRISPR Plasmids - Empty gRNA Vectors

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    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...genome engineering from a single lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...time. Single plasmids containing both the gRNA and a Cas protein act as an all-in-one vector, but their ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...

  4. Kazuhiro Oka Lentiviral Vectors

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    ...Collections Gene Vector Core Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral...information on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral Vector Guide ...series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this...Guide Find more plasmids in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog: Your... transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Depositor... this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've ...

  5. Lentivirus Plasmids

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    Collection
    ...safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express...Lentiviral Plasmids Transfer Plasmids Packaging Plasmids Envelope Plasmids Resources Lentiviruses, a subset...Resources Lentiviral Vector Guide Addgene’s Viral Service Lentiviral Pooled Libraries All Viral Plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral...Read our Lentiviral Vector Guide for more information about lentiviral components, generations, viral ...Browse our catalog for lentiviral CRISPR plasmids for gRNA expression . ID Plasmid Generation Description...Feng Zhang Return to top Popular Lentiviral Packaging Plasmids ID Plasmid Generation Description PI 1732...

  6. Viral Production

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    Collection
    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...virus protocols . Browse our plasmid collection of popular lentiviral vectors .... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....-free plasmid purification protocol. To minimize the immunogenic properties of the final vector preparation...

  7. Luciferase Plasmid Collection

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    Collection
    ...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription... Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream... regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...Janes 99283 pCW57.1-Luciferase Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression...

  8. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing...2 Pseudotyped Virus SARS-CoV-2 Spike Plasmids Accessory Plasmids Other Resources Mechanisms of SARS-CoV...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin...

  9. Tetracycline Inducible Expression

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    Collection
    ...Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...tet-controlled gene of interest from one plasmid. A single vector approach may not have as much flexibility...Highlighted Plasmids Browse the tables below to find popular empty backbones, expression vectors for transactivators...transactivators, and vectors for other applications, or search our collection for all dox-regulated plasmids . Looking...Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...

  10. Mammalian RNAi Tools

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    Collection
    ...delivery of a plasmid. shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources...a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression...argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...

  11. Lentiviral Prep Service

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    ...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our repository...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository ...

  12. Viral Vector Guides and Plasmids

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    ...Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile ...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ... Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to...Browse viral vector guides and plasmids available at Addgene and other related resources. Science...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...These vectors rely on production plasmids that have been carefully optimized for both safety and efficiency...

  13. Plan Your Experiment

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    Collection
    ...viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral vectors... in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. ...'s collection of CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are a great option, as they...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...

  14. Zhang Lab CRISPR Page

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    Collection
    ...Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422...VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper...guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses S. pyogenes Cas9...SpCas9 plasmid series. ), and a pair of annealed oligos can be cloned scarlessly into the vector before...

  15. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+... Ligand Plasmids HaloTag Chloralkane (CA) pMH-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag...Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus Expression pSNAP-tag (T7) Vector - Bacterial...pCLIP-tag (m) Vector - Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag...Neveu Lab MXS Chaining Kit Luciferase Plasmid Collection Promega Plasmid Collection Reading and Resources ...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  16. CRISPR Plasmids and Resources

    Type
    Collection
    ... of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...

  17. Adenovirus Plasmids

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    Collection
    ...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

  18. CRISPR Guide

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    Collection
    ...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease ... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous... Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered...Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...

  19. CRISPR References and Information

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    Collection
    ...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 ... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new...

  20. Neurodegeneration Research Collection

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    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids provided by the foundation...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...
Showing: 1 - 20 of 26 results