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We narrowed to 27 results for: lentiviral vector plasmid

Showing: 1 - 20 of 27 results

  1. Plasmids for Stem Cell Research

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    ... Plasmid Collections Plasmids for Stem Cell Research Plasmids for Stem Cell Research...Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids Stem cells... table based on plasmid name, species, gene name, and more. Plasmid Gene/Insert Vector Type PI Publication... 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector for the expression of human.... 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector for the expression of human...-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the expression...151-9. Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...

  3. CRISPR Plasmids - Empty gRNA Vectors

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    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...genome engineering from a single lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9...single gRNA vectors with various promoters, plasmids for assembling multiple gRNAs into one vector, and plasmids...Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit...time. Single plasmids containing both the gRNA and a Cas protein act as an all-in-one vector, but their ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited...

  4. Kazuhiro Oka Lentiviral Vectors

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    ...Viral Vectors Lentivirus Oka Lab Lentiviral Vectors Baylor College of Medicine Gene Vector Core Lentiviral...Adenoviral Vectors You may also like... Lentiviral Guide Adenoviral Guide Viral Vector Plasmids and Resources...Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource Guide Lentiviral Transfer Vectors Adenoviral Vectors...series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Viral... this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've ...these transfer vectors and generating infectious virus. Protocol: Lentivirus Transfer Vector Production Protocol...

  5. SARS-CoV-2 Pseudotyped Virus

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    ...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...activity are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc...Retroviral reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter...reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...

  6. Viral Production

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    ...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...virus protocols . Browse our plasmid collection of popular lentiviral vectors .... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....-free plasmid purification protocol. To minimize the immunogenic properties of the final vector preparation...

  7. Luciferase Plasmid Collection

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    ...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription... Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream... regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...Janes 99283 pCW57.1-Luciferase Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase expression...

  8. Tetracycline Inducible Expression

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    ...Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...tet-controlled gene of interest from one plasmid. A single vector approach may not have as much flexibility...Highlighted Plasmids Browse the tables below to find popular empty backbones, expression vectors for transactivators...transactivators, and vectors for other applications, or search our collection for all dox-regulated plasmids . Looking...Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...

  9. Lentivirus Plasmids

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    ...Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer ... Viral Plasmids Lentiviral Plasmids Lentiviral...of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...Looking for lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table... Also see plasmid 46948 . Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based...

  10. Mammalian RNAi Tools

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    ...delivery of a plasmid. shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred...BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a ...pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources...a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression...argonaute, please search our full site . ID Plasmid Vector Type PI Publication Additional Resources Addgene...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...

  11. Lentiviral Prep Service

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    ...more details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled... list of gRNA plasmids targeting specific genes. For more information on CRISPR plasmids and function,...Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau Viral... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in our repository...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository ...

  12. Plan Your Experiment

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    ...viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral vectors... in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. ...'s collection of CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are a great option, as they...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes...design. Addgene offers many plasmids containing validated gRNAs . These plasmids contain gRNAs that have ...generate stable cell lines if your plasmid contains a selection marker. Plasmid delivery is versatile thanks...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...

  13. Zhang Lab CRISPR Page

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    ...Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested... in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9...of 3 vectors each available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422...VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper...guide RNA (sgRNA) into one plasmid (Ran et al., Nature 2015). The dual vector system uses S. pyogenes Cas9...SpCas9 plasmid series. ), and a pair of annealed oligos can be cloned scarlessly into the vector before...

  14. Fluorescent Protein Guide: Empty Backbones

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    ... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression...mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+...Species) Organism/Expression System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP...Verkhusha Lab Plasmids - Enhanced colors, including PSmOrange, iRFP Gradia Lab Mammalian Plasmids - Includes...Lab Plasmids - Includes many N- and C-terminal fluorescent proteins Insect Sutherland Lab Plasmids - For...expression Davidson Lab Plasmids - Includes many fluorescent proteins in the pBAD vector Jump to Top References...

  15. Viral Prep Service for Plasmids and Pooled Libraries

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    ...technologies. Penn Vector Core AAV Vectors Many AAV vectors from the Penn Vector Core have been transferred to Addgene...Viral Vector Packaging Service Addgene produces high-quality viral vector preps from select plasmids in ...eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request Viral Vector Packaging.... We know that viral vector production is a common step after requesting plasmids from Addgene, and by...In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer preps...about viral vector production at Addgene, ordering information, and more. Additional Viral Vector Resources...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...

  16. CRISPR Plasmids and Resources

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    ... of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used together...gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety ...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety.... elegans CRISPR plasmids designed for use in C. elegans . Drosophila CRISPR plasmids designed for use...Mammalian CRISPR plasmids designed for use in mammalian systems. Parasites CRISPR plasmids designed for use...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...

  17. Adenovirus Plasmids

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    ...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

  18. Retrovirus Plasmids

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    ...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

  19. CRISPR Guide

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    ...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous... Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered...Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...

  20. Neurodegeneration Research Collection

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    ... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...Subcellular Localization, Optogenetics Plasmids, and Chemogenetics Plasmids. New and Noteworthy: Explore NeuroPAL...Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids provided by the foundation...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...
Showing: 1 - 20 of 27 results