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We narrowed to 13 results for: expression vector

Showing: 1 - 13 of 13 results

  1. Plan Your Experiment

    Type
    Guide
    ...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes a bit more prep work...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific...and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and...

  2. Adenovirus Guide

    Type
    Guide
    ...Guides Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production Integration...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...adenoviral (rAdV) vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus...Generations First-generation rAdV vectors The first generation of rAdV vectors was developed by removing the...other means. In order to produce rAdV vectors, E1 needs to be expressed by the packaging cell line, normally...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Guides AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...used as viral vectors for gene therapy applications. This guide contains many viral vector-specific terms...adenovirus, read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified...Plasmids required for rAAV vector production. Created with BioRender.com. AAV Vector Production Cloning Cloning...laboratory. The cell line used for rAAV vector production needs to express the adenovirus E1 gene, which is ...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral Vectors Vector Production...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...long-term expression in a host and low immunogenicity. This guide contains many viral vector-specific ...Gamma-retroviral vectors do not have defined “generations” of plasmids, as lentiviral vectors do. For more...lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral Vectors The genome of gamma-retroviruses...Addgene’s viral vector protocols . Production Using Packaging Cell Lines Gamma-retroviral vectors can be packaged...

  5. Molecular Biology Reference

    Type
    Guide
    ...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors...amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...for the transcription process to stop. An expression vector can also include an enhancer sequence which...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA...combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations Vector Production...Lentiviral Vectors Due to their integration and long-term expression of a transgene, lentiviral vectors are ...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have ...models. Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host, and...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically...

  7. Chemogenetics Guide

    Type
    Guide
    ... Dlx Interneurons EF1a, CAG General expression Targeted expression. Depending on the experiment, an AAV-encoded...which they drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells... our AAV guide . FLEx Vectors are also used to achieve cell-specific expression of AAV-encoded chemogenetic...chemogenetic receptors. FLEx vectors block expression in the absence of Cre and are especially useful in AAV...Want to know more about FLEx vectors? Read our Plasmids 101: FLEx Vectors blog! Download our chemogenetics.... The opsin may excite or inhibit the neurons expressing the LMO, depending on its biophysical properties...more than one domain, both of which need to be expressed. However, neuronal control through PSAMs and LMOs...

  8. Optogenetics Guide

    Type
    Guide
    ...ways to control opsin expression. Generally, the most robust and stable expression is achieved in a transgenic...fiber. Using viral vectors for opsin delivery results in more localized expression of the opsin. Depending...site of the viral vector, rather than in all cells. For example, using a viral vector with a floxed opsin...procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). When exposed to light of...as those described below, can be targeted and expressed in specific subsets of neurons, allowing precise...population is manipulated in a given experiment: the expression of the opsin and the area that is being illuminated...for example the VGAT-ChR2 mouse, where ChR2 is expressed under the control of the vesicular gamma aminobutyric...

  9. CRISPR Guide

    Type
    Guide
    ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...tools require constitutive expression of the dCas9 fusion and/or expression of multiple fusions. The effects...sequences . Once you’ve selected your target, you’ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein...the DSB site. Therefore, a population of cells expressing Cas9 and a gRNA will result in a diverse array...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing ...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

  10. Promoters

    Type
    Guide
    ...initiated. Promoters are a vital component of expression vectors because they control the binding of RNA polymerase...where in the organism your gene of interest is expressed. Summary Promoters are about 100-1000 base pairs...Eukaryotic Promoters Used in Research Promoter Expression Description CMV Constitutive Strong mammalian...Constitutive Human U6 nuclear promoter for small RNA expression UAS Specific Drosophila promoter containing Gal4...Bacterial Promoters used in Research Promoter Expression Description T7 Constitutive but requires T7 RNA...

  11. Cloning

    Type
    Guide
    ...them into plasmids for replication and protein expression. So how do scientists recombine DNA? There are...majority of plasmids used for DNA cloning and expression contain several popular restriction enzyme sites...shuttled into any compatible Gateway® Destination vector, which contain attR sites via LR clonase enzymes...a simple and efficient cloning method, gateway vectors and recombination enzymes can be quite expensive...comes from a pre-cut, linear, cloning ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ ...ready, and it is not feasible to create a TOPO® vector yourself. Additionally, the efficiency can vary...overhangs of 10-12 base pairs on the 5' end of both the vector and insert. These overhangs can easily anneal creating...

  12. Science Guides

    Type
    Guide
    ...Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune system, antibodies...given library have the same backbone, but they express or target different genes. Some libraries cover...restricted to certain gene sets. Read More Viral Vectors Find educational guides to learn more about the...

  13. Guide to Using Pooled Libraries

    Type
    Guide
    ...given library have the same backbone, but they express or target different genes. Some libraries cover...like lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen...genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our blog posts featuring ...
Showing: 1 - 13 of 13 results