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Addgene

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We narrowed to 11 results for: organization Addgene

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  1. Plan Your Experiment

    Type
    Guide
    ...these principles apply to using CRISPR in other organisms. First, consider the genetic manipulation that...carry out your experiment in your particular model organism. There may not be a perfect plasmid for your specific...disrupt gene function in a particular cell type or organism without a specific preferred mutation Cas9 (or...effects Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic...sequence and design your gRNA. Know your cell line/organism and genomic sequence When possible, you should...may vary depending on the specific cell line or organism, which may affect the observed efficiency of CRISPR...containing validated gRNAs are now available through Addgene. These plasmids contain gRNAs that have been used...

  2. Molecular Biology Reference

    Type
    Guide
    ...transcriptional machinery from a particular organism or group of organisms. Meaning, if a plasmid in intended ...videos for specific techniques and tips! Addgene Protocols Addgene Video Library...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing...abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix. The order of these...the instructions to make every protein within an organism. Proteins are made up of amino acids. Each amino... plasmid. For commonly used primers check out Addgene's sequencing primer list. Working with Plasmids ...can drive gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...by visiting Addgene's Optogenetics Guide . Browse optogenetics plasmids available at Addgene. CRISPR/Cas9...Vectors 101 Youtube Videos Addgene's Biosafety Resources Resources on Addgene's Blog Parts of the AAV Transfer...In-Stock AAV Preps Resources on addgene.org AAV Plasmids Available at Addgene Viral Vectors 101 eBook Viral...glossary at the end! Browse AAV plasmids available at Addgene. Adeno-Associated Virus versus Adenovirus Adenoviruses...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . Production The three...vivo models. For more detailed protocols, see Addgene’s Viral Vector Protocols . Figure 3: Overview of...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...

  4. Promoters

    Type
    Guide
    ...Addgene's molecular biology reference material covering promoters, transcription and the transcription...promoter region controls when and where in the organism your gene of interest is expressed. Summary Promoters...small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including our popular Plasmids 101 series...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...can find more information in Addgene’s stable cell line protocol . Addgene’s protocol is for lentiviral ...Vector topics Addgene protocols Generating Stable Cell Lines Using Lentiviral Vectors Addgene's Viral Protocols...Resources on addgene.org Gamma-retroviral Plasmids Collection Viral Vectors 101 eBook Addgene's Biosafety...the end! Browse retroviral plasmids available at Addgene. Gamma-Retrovirus Versus Lentivirus Lentiviruses... vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral Vectors...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . Production Using...viral titer. For more detailed protocols, see Addgene’s viral vector protocols . Production Using Packaging...

  6. Chemogenetics Guide

    Type
    Guide
    ...Addgene's guide to using Chemogenetics plasmids in your lab for interrogation of neuronal activity.... find chemogenetics plasmids available through Addgene, check out our Chemogenetics Plasmid Collection...that both KORD and hM3Dq can be used in the same organism to allow bidirectional control of neuronal activity...alternative DREADD ligands, please see the text. Browse Addgene's collection of DREADD plasmids ! PSAMs Unlike DREADDs...Neuronal activation Magnus et al., 2011 Browse Addgene's collection of PSAM plasmids ! LMOs Luminopsins...Neuronal inhibition Ikefuama et al., 2025 Browse Addgene's collection of LMO plasmids ! Planning Your Chemogenetics...

  7. Optogenetics Guide

    Type
    Guide
    ...Addgene's guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin...To find optogenetics plasmids available through Addgene, check out our Optogenetics Plasmid Collection ...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...previous tools through directed mutagenesis - use Addgene's Optical Switch Plasmid Table to search for plasmids...

  8. Adenovirus Guide

    Type
    Guide
    ...Viral Vectors 101 eBook Addgene's Biosafety Resources Resources on Addgene's Blog An Introduction to Adenovirus... References Resources on addgene.org Adenoviral Plasmids Available at Addgene AdenoBuilder Toolkit Viral...the end! Browse adenoviral plasmids available at Addgene. Adenovirus versus Adeno-Associated Virus Adenoviruses...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . AdEasy™ AdEasy™,...construct is then transformed into AdEasier-1 cells (Addgene #16399) , which are BJ5183 E. coli cells containing...viruses. The adenoviral backbone plasmid pAdEasy-1 (Addgene #16400) is suitable for most purposes. For especially...especially long transgenes, the use of pAdEasy-2 (Addgene #16401) can increase the capacity of the rAdV vector...

  9. Lentiviral Vector Guide

    Type
    Guide
    ...vectors can be found in Addgene’s stable cell line protocol . Browse Addgene's collection of in-stock ... Viral Vectors 101 eBook Addgene's Biosafety Resources Resources on Addgene's Blog Quick Guide to All ...Resources on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral Preps Addgene's Viral Prep ...the end! Browse lentiviral plasmids available at Addgene. Lentivirus Versus Gamma-Retrovirus Lentiviruses...versa. For more about gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors...information on cloning and working with plasmids, visit Addgene’s Molecular Biology Reference . Production After...a transgene. For more detailed protocols, see Addgene’s viral vector protocols . Figure 5: Overview of...

  10. CRISPR Guide

    Type
    Guide
    ...window) . Addgene Website & Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101...cell lines Viral service at Addgene Viral plasmids and resources at Addgene Blog CRISPR topic page How ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...You Use a CRISPR Library? CRISPR libraries from Addgene are available in two formats: as liquid DNA, or...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...located. The majority of the CRISPR plasmids in Addgene’s collection are from S. pyogenes unless otherwise...

  11. Antibody Guide

    Type
    Guide
    ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...intensive. Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made...myeloma cells (immortalized B cells). The genetic reorganization that creates variety in the antibody allowing...the variable region goes through a randomized reorganization process, creating a unique binding site. This...
Showing: 1 - 11 of 11 results