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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 38 results for: clu

Showing: 1 - 20 of 38 results

  1. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...pathogens including organisms such as Staphylococcus aureus or Vibrio cholerae . BSL-2 includes all of the...sharps container. Sharps include anything that can pierce your skin, including, but not limited to broken...experiment, make sure your workspace is clean and uncluttered. Disinfect your workspace with appropriate materials... all areas containing biohazardous materials, including biohazardous waste. Dispose of waste that may ...followed in addition to BSL-2 guidelines below, including PPE protocols . Working in a BSL-2 laboratory ...biohazardous waste containers that can be autoclaved. Conclusion Although simple, following appropriate BSL-1 ...

  2. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...and samples in duplicate at least If possible, include an AAV reference sample of known titer. The reference...expected titer of the samples being tested. Always include a No Template Control (NTC), i.e master mix + water... of each sample in duplicate. Do not forget to include a no template control ( NTC = master mix + water...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than ...

  3. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Other methods of generating a monoclonal cell line include single-cell sorting (Link opens in a new window... protocol will vary based on several factors, including the growth properties of the cell lines, the effects...cell pool by trypsinization and break up any cell clumps into individual cells by passing several times ...Wild-type A549 cells do not express Cas9 and are included as a control (WT). Vinculin is a loading control...

  5. Ligation Independent Cloning

    Type
    Protocol
    ...In this case, we will include the free nucleotide dGTP in the reaction (exclude all other nucleotides ...chew back" reaction, in which a single dNTP is included to stop the exonuclease function of the enzyme...

  6. Gibson Assembly Protocol

    Type
    Protocol
    ...used when the part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short...stitching” primers and the amounts of primers to include in the Gibson reaction are different than with ...window) sells DNA Assembly kits and master mixes, including NEBuilder HiFi (Link opens in a new window) and...

  7. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ...the bacteria to lyse, releasing their contents, including plasmid DNA, into solution. Add a renaturing solution...step 6 to eliminate RNA contamination. This is included in the resuspension buffer of most kits. Reference...

  8. Protocol - How to Design Primers

    Type
    Protocol
    ... a certain position within the primer, one can include more than one nucleotide at that position termed...have complementary regions Note: If you will be including a restriction site at the 5’ end of your primer...

  9. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...Protocol for creating a glycerol stock, including additional tips for long-term storage....the appropriate temperature. Growth conditions, including copy number and growth temperature, can be found...

  10. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

  12. Weighing Reagents Protocol

    Type
    Protocol
    ... You need to do this because you don’t want to include the weight of the weighing boat or paper into your...paper in the biohazard solid waste container. Conclusion Appropriately weighing reagents is important ...

  13. Video Library

    Type
    Protocol
    ...A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and...some of the key strategies of aseptic technique, including personal protective equipment, setting up a clean...

  14. Immunocytochemistry

    Type
    Protocol
    ... or recommend specific products or equipment. Inclusion of this information is solely for transparency... is both functioning as expected and specific, include a positive control sample that you know expresses...

  15. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

  18. Western Blot

    Type
    Protocol
    ... or recommend specific products or equipment. Inclusion of this information is solely for transparency... both functioning as expected and is specific, include a positive control sample that you know expresses...

  19. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... or recommend specific products or equipment. Inclusion of this information is solely for transparency... of the standard curve you will need to either include higher concentrations in the standard curve or ...

  20. Kit Free RNA Extraction

    Type
    Protocol
    ...without a kit. The general steps of this protocol include homogenization/lysis of cells or tissues, extraction...For the precipitation step, two options are also included: using Isopropanol or Lithium Chloride. This protocol...
Showing: 1 - 20 of 38 results