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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16 results for: clu

Showing: 1 - 16 of 16 results

  1. CRISPR Guide

    Type
    Guide
    ...Each system also includes Tn7-like transposase components. Type I (generally) includes tnsA , tnsB , tnsC...applications to increasingly complex functions, including the ability to selectively activate/repress target...carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual nickases...increased fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the...SpCas9-derived variants with non-NGG PAM sequences include: xCas9 - NG, GAA, and GAT; increase nuclease fidelity...adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and...members inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition; inhibiting...

  2. Molecular Biology Reference

    Type
    Guide
    ...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing...years, many significant discoveries lead to the conclusions that genes encode proteins and reside on chromosomes...essential DNA sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance...whose product confers resistance to ampicillin) is included in the plasmid. These bacteria are then grown ...in molecular biology for a variety of reasons, including that they are: Easy to work with - Plasmids are...gene expression in a wide variety of organisms, including plants, worms, mice, and even cultured human cells... a DNA fragment. Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors....

  3. Lentiviral Vector Guide

    Type
    Guide
    ... many species. Some common types of lentivirus include Human immunodeficiency virus (HIV), bovine immunodeficiency...viral vectors or simply need a refresher, we've included a glossary at the end! Browse lentiviral plasmids...remains largely intact. First-generation plasmids include (Figure 2): Transfer plasmid — contains transgene...the accessory genes. Second-generation plasmids include tat , as the 5’ LTR is used as a promoter on the...Tat for activation. Second-generation plasmids include (Figure 3): Transfer plasmid — contains transgene...transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — contains transgene...ONLY by a second-generation packaging system that includes the tat gene Can be packaged by both second- and...

  4. Optogenetics Guide

    Type
    Guide
    ...guide to using Optogenetics plasmids in your lab, including explanations of microbial opsin and optical switch... is a list of commonly used microbial opsins, including a brief overview of the wild-type opsin of each...functionality of ChR. Examples of ChRs from other species include: CsChR (from Chloromonas subdivisa ), CoChR (from...depolarization) of the neuron. Feature enhancements include: Increased photocurrent amplitude Examples: ChR2...Examples of anion channel variants from other species include: GtACR1 and GtACR2 (from the cryptophyte Guillardia...Guillardia theta ). Other feature enhancements include: Increased photocurrent amplitude Examples: iChloC,...Variants. Mac variants have been engineered to include enhancements such as: Improved photocurrent amplitude...

  5. Educational Resources

    Type
    Guide
    ...content , including protocols, tips on using Addgene’s materials, and career advice. Playlists include: Protocols...Overview of Addgene's educational resources, including eBooks, science guides, videos, and protocols ...popular topics related to our collections. Titles include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent...and related molecular biology techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR...assist with your molecular biology research. Topics include: Antibiotic Concentrations for Bacterial Selection...molecular biology, plasmid cloning, and virus, including: Gel Electrophoresis Bacterial Transformation ...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... AAVs are part of the Dependoparvovirus genus, included within the Parvoviridae family. They can infect...viral vectors or simply need a refresher, we've included a glossary at the end! Browse AAV plasmids available...interest. The size of the genetic cargo that can be included between the ITRs is limited by the physical space...can be done with most standard cloning methods, including restriction enzyme, Gibson Assembly, or Gateway...large amounts of rAAV vectors. The E1 gene is not included in the helper plasmid and instead provided by ... applications due to their unique properties, including their non-pathogenic nature, ability to target...brain through neuronal tracing with rAAV vectors include the Allen Mouse Brain Connectivity Atlas and the...

  7. Antibody Guide

    Type
    Guide
    ...2) to completely unrelated molecules. Examples include: scFv - Single-chain fragment variables (scFvs)... used to quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate ...isolate specific proteins or protein complexes. Includes: Immunoprecipitation (IP) Chromatin immunoprecipitation...Visualization - used to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry ...identify, analyze, capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the...the applications common to each category, including an outline of the basic steps needed for each application...run. The standard curve should contain a range inclusive of all expected protein concentrates in the sample...

  8. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...associated with many serious illnesses. Common types include murine leukemia virus (MLV), murine sarcoma virus...viral vectors or simply need a refresher, we've included a glossary at the end! Browse retroviral plasmids...can be done with most standard cloning methods, including restriction enzyme, Gibson Assembly, or Gateway... type, there are other envelope gene options, including: Gibbon Ape Leukemia Virus (GALV) — T and B cell...

  9. Plan Your Experiment

    Type
    Guide
    ...expanded the CRISPR toolbox to include many different types of edits, including point mutations, base changes...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...VP64) Many different activator systems exist, including the multi-plasmid SAM system Activate Design and...

  10. Adenovirus Guide

    Type
    Guide
    ...viral vectors or simply need a refresher, we've included a glossary at the end! Browse adenoviral plasmids...interest. The size of the genetic cargo that can be included between the ITRs is limited by the physical space...region from the viral genome. This allowed for the inclusion of ~6.5 kb of genetic cargo in first-generation...can be done with most standard cloning methods, including restriction enzyme, Gibson Assembly, or Gateway...

  11. Promoters

    Type
    Guide
    ...controlled by various DNA regulatory sequences including enhancers, boundary elements, insulators, and ...of gene regulation, like operons. Operons are a cluster of different genes that are controlled by a single... shRNAs and gRNAs. Resources Addgene's blog , including our popular Plasmids 101 series , covers topics...

  12. Chemogenetics Guide

    Type
    Guide
    ...involved in a wide variety of biological processes, including initiating signaling pathways in inflammation ... less than ideal. DREADDs Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), like RASSLs... Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs. ACS Chem Neurosci...

  13. Science Guides

    Type
    Guide
    ...of viruses that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus...

  14. Addgene's eBook Collection

    Type
    Guide
    ...November 2018 | First Edition About The guide includes information on the many career options in science...

  15. Guide to Using Pooled Libraries

    Type
    Guide
    ...selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer drug vemurafenib...

  16. Cloning

    Type
    Guide
    ...reference guide on molecular cloning techniques, including information on seven commonly used cloning methods...
Showing: 1 - 16 of 16 results