We narrowed to 35 results for: iCre

... Protocols Using a Light Microscope Using a Light Microscope Intro to the Lab...eye, like microbes in a drop of pond water or the cells in your cell culture dish. Microscopes come in ...encounter: the compound light microscope. As the name suggests, light microscopes take advantage of the physical...light microscope with the main components labeled: Figure 1: Diagram of a compound light microscope with...Equipment Light Microscope Prepared Slides (or other sample that can fit on a microscope stage) Reagents...you are using the microscope you may wish to record your observations. Some microscopes have digital cameras...take care of your microscope. Bumps, scratches, and dust can easily impair microscope performance. Be sure...

...controller Microcentrifuge Microplate shaker ELISA plate washer (optional) Scale Microsoft Excel or similar...well polyester (clear) microplate seal, Thermo Scientific 5701 Microcentrifuge tubes, Neptune 3745.X Pipette...isotype-specific secondary antibody ELISA 96-well microplate, Corning 9018 Bovine serum albumin (BSA), VWR...plate Dilute purified antigen to 20 ng/µL in a microfuge tube and vortex. Pro-Tip You may need to try a...100 µL of 20 ng/µL stock into 900 µL PBS in a microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/...ng/µL stock into 450 uL PBS in a microfuge tube and vortex. 0.5 ng/µL : Add 450 µL of 1 ng/µL stock into...into 450 µL PBS in a microfuge tube and vortex. 0.25 ng/µL : Add 450 µL of 0.5 ng/µL stock into 450 µL ...

... of an open tabletop microcentrifuge with the lid open, the rotor with microfuge tubes in place, and the...samples in 1.5 mL microfuge tubes at 10,000 rpm would require a tabletop microcentrifuge like the one in...Last Update: September 2022 Equipment Centrifuge Microfuge tubes or other suitable container Personal protective...benchtop and typically hold the common 1.5 mL microfuge tubes used in labs, though some larger models ...based on weight instead of volume. Pro-Tip For microfuge tubes, place them with the lid tabs pointing up...

...the stock culture to a 1.75 mL microfuge tube. Centrifuge in microfuge tube at 10,000 g for 30 sec. Pour...isolating plasmid DNA in quantities as low as a few micrograms to as much as several miligrams and at concentrations...Last Update: Feb. 8, 2018 Equipment Desktop microcentrifuge Desktop vortexer Vacuum (optional) Reagents...used if TE-saturated is not available. Vortex microfuge tube for 30-60 sec. Centrifuge the tube for 5 ...Pipet the aqueous DNA layer and place it in a new microfuge tube. Add equal volume of chloroform to the recovered...volume of 3 M Na-acetate (pH 4.8). Invert the microfuge tube to mix. Optional: Place the tube either at...

...mounting medium to the microscope slide. Gently place the coverslip on the microscope slide with the cell...pipettes Rocking platform Tweezers Fluorescent microscope 0.5–10 µL single channel pipette 2–20 µL single... µL single channel pipette Reagents 1X PBS Microcentrifuge tubes Sterile Poly-D-lysine coated coverslips...Primary antibody Secondary antibody Deionized water Microscope slide Anti-fade mounting medium Laboratory wipes...side facing down. Observe the cell labeling on a microscope with appropriate fluorescent filters. Tips and...

... combs Voltage source Gel box UV light source Microwave Reagents TAE ( recipe here ) Agarose Ethidum bromide...). Mix agarose powder with 100 mL 1xTAE in a microwavable flask. See TAE Recipe . Pro-Tip TBE can be used... mix different buffers and do not use water). Microwave for 1-3 min until the agarose is completely dissolved... of agarose in the gel. Many people prefer to microwave in pulses, swirling the flask occasionally as ...boiling can occur. Pro-Tip It is a good idea to microwave for 30-45 sec, stop and swirl, and then continue... 10% more volume than needed because several microliters can be lost in pipetting. For example, if you...

... single channel pipette Pipette controller Microcentrifuge Electrophoresis chamber Power supply Rocking...VWR 89130-902 Gel loading tips, Corning 4853 Microcentrifuge tubes, Neptune 3745.X PBS, 1X pH 7.4, VWR 45000...10 µL PBS Transfer 5 µL of each standard to a microfuge tube. Add 8 µL of PBS to a total volume of 13 ...mL. Transfer 5 µL of recombinant antibody to a microfuge tube. Add 8 µL of PBS to total 13 µL . Prepare...each sample. Spin the sample briefly in the microcentrifuge. Heat the samples for 10 min at 100 °C in a...heat block. Spin the sample briefly in the microcentrifuge. Remove the gel from the plastic wrapper and...

...protein from cells or tissue samples. Equipment Microcentrifuge 0.5–10 µL single channel pipette 2–20 µL single...1X PBS Lysis buffer e.g., RIPA lysis buffer Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol...IB24002 20X TBS Tween-20 Nonfat milk powder 96-well microtiter plate Chemiluminescence substrate Plastic wrap...pellet in 1 mL of 1X PBS and transfer to a microcentrifuge tube. Centrifuge for 5 min at 100 x g . Carefully... at 4 °C . Transfer supernatant to a clean microcentrifuge tube. Use the lysate immediately or store at...samples into 200 µL of BCA reagent in a 96-well microtiter plate. Incubate for 30 min at 37 °C . Determine...

... Table top centrifuge BioRad Electroporator (MicroPulser TM , Bio-Rad 1652100) Reagents 200 µL electrocompetent...the pool. 8 electroporation cuvettes (BioRad, Micropulser, 0.1 cm ) 20 mL SOC recovery media (Lucigen, ... Agar + Antibiotic Bioassay plates. Prechill Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent...until completely thawed. Chill a box of 200 µL micropipette tips in a -20 °C freezer. Aliquot 3 mL SOC into...electroporations): Electroporator Conditions: Bio-Rad Micropulser Ec1 0.1 cm cuvette, 1.8 kV, 1 pulse. Aliquot .... Typically yields range from several hundred micrograms to a milligram of total plasmid DNA. Tips and...

...Equipment Biosafety Cabinet 4 °C Refrigerator Micropipettes and tips Pipettor and pipettes Benchtop centrifuge...DNA 500 mL vented flask 50 mL conical tubes Microcentrifuge tubes Cell counting chamber slide 30 mL luer-lock... mL of HEK293 cell suspension into a clean microcentrifuge tube. Pro-Tip Cells settle quickly and need...Transfer 10 µL of trypan blue into a clean microcentrifuge tube. Vortex the cell suspension. Transfer ...Transfer 10 µL of cell suspension into the microfuge tube containing the trypan blue. Pipette 10 times to mix...

...MLL4801 Pierceable Foil Heat Seal, Bio-Rad, 1814040 Microseal adhesive seal, Bio-Rad, MSB1001 Polystyrene Reservoirs...Reservoirs, VWR, 89094-662 Microcentrifuge tubes, VWR, 87003-294 Primers/probe targeting ITR: ITR Forward...above). Save ~25 µL of 1X dilution buffer in a microcentrifuge tube to be used as the NTC. Pour the 1X dilution.... Gently cover the entire dilution plate with Microseal adhesive seal - do not press the film, just gently...BSC to cool. Prepare the ITR master mix in a microcentrifuge tube as shown below. For 8 samples prepare ...

... Biological Safety Cabinet Aspirating unit Microcentrifuge, Eppendorf, 5425R Droplet digital PCR System...1814040 Polystyrene reservoirs, VWR, 89094-662 Microcentrifuge tubes, VWR, 87003-294 Primers/probe targeting...series of virus in DMEM complete: Prepare 7 microcentrifuge tubes with the appropriate amount of media ... in 500 µL DMEM complete and transfer to a microcentrifuge tube. Centrifuge for 5 min at 100 x g . Gently...cool. Prepare the RRE/RPP30 master mix in a microcentrifuge tube as shown below. For 8 samples prepare ...

...selection markers. Note, because this protocol uses microscopy to determine the percentage of fluorescent cells...Hazardous waste container Inverted fluorescence microscope Reagents DMEM high glucose, Corning 10-013-CV...preps, if prep was not previously filtered) Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture...assayed for mCherry expression using fluorescence microscopy. Last reviewed on: September 9, 2023...

...#1 Prepare a row of roughly 20 open 1.5 mL microcentrifuge tubes in a rack. These will be used to collect...Figure 2) with an 18 ga needle. Place the first microcentrifuge tube under the needle’s opening to collect ...at the 40–60% interface. Make sure that the microcentrifuge tubes are well positioned to collect the solution... start collecting 0.5 to 1 mL fractions in microcentrifuge tubes. Repeat for each QuickSeal tube. The ...

...incubator Pipet controller Hazardous waste container Microscope Hemocytometer or other cell counter Reagents ...with ~1000 cells. This will help you focus the microscope later when scanning the plate for individual ...Pro-Tip Use the well with 1000 cells to focus the microscope, then you can easily scan the plate for individual...trypsinizing cells from the 96-well plate, use a microscope to ensure that the cells have detached from the...

...container Containers to hold measured liquid (ex: microfuge tube, bottle, etc.) Labels for containers Reagents...using to hold the measured liquid (ex: another microcentrifuge tube, a bottle etc.). If there is already other...liquid into different tubes or into wells of a microplate, you may consider using a multichannel pipette...

...Equipment Refrigerated microcentrifuge If you only have a non-refrigerated microcentrifuge, see step 4 of either...RNase-free water or TE solution RNase free tubes: microcentrifuge tubes, 4 mL polypropylene tubes RNase decontamination...

...liquid remaining on the surface). We use the micropipette tip itself to do the spreading; the tip is gently...same way as the antibiotic, using either a bent micropipette tip or other cell spreading device that fits...

... gel per reaction. Place the gel in a labeled microfuge tube. Using a scale, weigh the tube with the gel... 10% more volume than needed because several microliters can be lost in pipetting. For example, if you...

...liquid sample Using a Light Microscope Learn about the parts of a light microscope and its use Weighing Reagents...