...bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria... and you will not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can...

...complete plasmid can be transformed into bacterial cells for propagation. The majority of ligation reactions...Interpretation Uncut vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid...and vector, and what ratio you want, and it will tell you exactly how much of each to use. Reference Page...

...like microbes in a drop of pond water or the cells in your cell culture dish. Microscopes come in a huge ...transmission electron microscopes that allow us to see cellular components with science-fiction levels of detail...

...England Biolabs (NEB) (Link opens in a new window) sells DNA Assembly kits and master mixes, including NEBuilder...Assembly (Link opens in a new window) . They also sell ET SSB (Link opens in a new window) . NEB has other...

...another. The size of the primer is very important as well. Short primers are mainly used for amplifying a ...

...clothing from potential spills. Gloves should fit well enough to be snug to your hand, but not so tight...

... a wider gel comb; or c) loading less DNA in the well. How do you get better separation of bands? If you...

...Sequence Scanner (PC), and Chromas (PC). How can I tell if a peak is mis-called? Open the trace file and...

...restriction digests needed in molecular biology, and thaw cell lines. There are many different types of water baths...

...the correct orientation. For detailed protocols as well as tips and tricks see the Restriction Digest and...

...same amount of liquid into different tubes or into wells of a microplate, you may consider using a multichannel...

...Note: (Link opens in a new window) Primer3 is an excellent resource for choosing primers. Place thin-walled...

... the plasmids of interest (for example, fewer satellite colonies will grow). However, it is also more ...