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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 49 results for: TRO

Showing: 31 - 49 of 49 results

  1. Lentivirus Production

    Type
    Protocol
    ...like... Viral Vector Guides Mol Bio Protocols Introduction This protocol can be used to produce lentivirus...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH meter Stir plate... should be aliquoted and snap frozen in liquid nitrogen and stored at -80 °C as soon as possible to avoid...

  2. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA Purification Streaking and ...Bacteria Inoculating a Liquid Bacterial Culture Introduction Many molecular biology techniques require highly...window) Qiagen , (Link opens in a new window) Invitrogen , and (Link opens in a new window) Promega sell...

  3. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...standard Polymerase Chain Reaction (PCR) is an in vitro method that allows a single, short region of a DNA...product can be verified based on size by gel electrophoresis . Protocol Video Watch the protocol video below...to each reaction. The ideal setup for this troubleshooting step is to do one reaction with each, and one...

  4. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Vector Guides Virus Blog Posts Mol Bio Protocols Introduction The AAV Titration protocol can be used to determine...samples being tested. Always include a No Template Control (NTC), i.e master mix + water Whenever possible...duplicate. Do not forget to include a no template control ( NTC = master mix + water ). Add 15 μL of Master...

  6. Protocol - How to Streak a Plate

    Type
    Protocol
    ...Recovering Plasmid DNA from Bacterial Culture Introduction If you have a glycerol stock or stab culture...would hold a pencil, so that you can make a broad stroke. Only touch the surface of the plate, do NOT dig...

  7. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Recovering Plasmid DNA from Bacterial Culture Introduction This protocol describes methodology for plating...concentrations of carbenicillin plated over-agar. Control Plate with No Carbenicillin Plate shows a lawn ...

  8. Weighing Reagents Protocol

    Type
    Protocol
    ...Protocols Weighing Reagents Weighing Reagents Protocol Intro to the Lab Bench Check out more protocols and videos...videos to help you get started in the lab! Introduction For many experiments, you’ll need to make buffers...

  9. Water Bath Protocol

    Type
    Protocol
    ... Protocols Water Bath Water Bath Protocol Intro to the Lab Bench Check out more protocols and videos...videos to help you get started in the lab! Introduction A water bath is a piece of laboratory equipment...

  10. General Transfection

    Type
    Protocol
    ...Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol describes a general method for...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...

  11. Pipetting Protocol

    Type
    Protocol
    ... Protocols Pipetting Pipetting Protocol Intro to the Lab Bench Check out more protocols and videos...videos to help you get started in the lab! Introduction The pipette is an essential tool for nearly anyone...

  12. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Vector Guides Virus Blog Posts Mol Bio Protocols Introduction This protocol can be used to purify AAV of any...VP2, VP3; M protein marker. Image adapted from Strobel Benjamin, Miller Felix D., Rist Wolfgang, and Lamla...

  13. AAV ddPCR Titration

    Type
    Protocol
    ...Virus Blog Posts Molecular Biology Reference Introduction This protocol describes how to use droplet digital... and positive droplets (blue). The no template control (NTC) should be close to zero (B08). At Addgene...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Virus Blog Posts Molecular Biology Reference Introduction This protocol can be used to produce AAV from... cell counter Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH meter Stir plate...

  15. Protocol - How to Design Primers

    Type
    Protocol
    ...Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide primers...

  19. Pouring LB Agar Plates

    Type
    Protocol
    ...Blog Posts Mol Bio Protocols Cloning Protocols Introduction Plasmids can carry one or more antibiotic resistance...
Showing: 31 - 49 of 49 results