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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 42 results for: ins

Showing: 31 - 42 of 42 results

  1. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...should be properly disposed of following your institution’s hazardous waste procedures. Last Update: February...February 7, 2022 Protocol Video Watch this instructional video to learn how to affinity purify recombinant...for your columns, refer to the manufacturer’s instructions. Attach the Gravitrap column to a clamp on a...

  2. Protocol - How to Design Primers

    Type
    Protocol
    ... also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing...

  3. Protocol - How to Streak a Plate

    Type
    Protocol
    ...of plasmids in your purified DNA. This protocol explains how to isolate a single bacterial colony by streaking...plasmids or bacteria need to be grown at 30 °C instead of 37 °C. This is often true for large unstable...

  4. Lentivirus Production

    Type
    Protocol
    ...Envelope plasmid, and Transfer plasmid encoding your insert of interest Reagent Preparation DMEM Complete (... optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...

  5. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: February... °C. See below for other Reagent Preparation instructions. Wipe down all pipettes and equipment with 10%...

  6. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...purification and isolation of cells, organelles, lipoproteins, and macromolecules. Importantly, iodixanol ...of contaminants in each fraction. * AAV capsid proteins VP1, VP2, VP3; M protein marker. Image adapted...

  7. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...cloning cylinders. Workflow Timeline This protocol begins with a stable cell pool. For details, see our protocol...homogenized cell solution to 990 µL complete medium. Instead of transferring 0.125 µL, now transfer 100 times...

  8. DNA Quantification

    Type
    Protocol
    ...you have a NanoDrop, follow the manufacturer's instructions for the spectrophotometer specific to your lab...

  10. Fluorescence Titering Assay

    Type
    Protocol
    ...ensure that you are in compliance with your institution’s biosafety regulations. Workflow Timeline Day...

  11. General Transfection

    Type
    Protocol
    ...µg of DNA into 500 µL of Opti-Pro SFM. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...

  12. Pipetting Protocol

    Type
    Protocol
    ...the liquid and avoid touching the pipette to the inside of the tube. Only the tip should be in the liquid...
Showing: 31 - 42 of 42 results