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  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ...multiple times to obtain a single cell suspension (no clumps of cells). Pool cells from 2 x T-175 flasks. Adjust...vigorously to obtain a single cell suspension (no clumps). Count cells using a hemocytometer or cell counter...

  2. CRISPR Library Amplification

    Type
    Protocol
    ...of Endura Duos, Lucigen, 60242-1) Alternatives include Stbl4 cells or other ultra-high efficiency electrocompetent..., gentler practices, and colder reagents (not including the recovery media!). Do not proceed with Maxipreps...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...efficiently infect a broad range of cell types, including both dividing and non-dividing cells. Addition...warranty of any kind either expressed or implied, including but not limited to the implied warranties of fitness...

  6. Centrifugation

    Type
    Protocol
    ...to save the pellet, the supernatant, or both. Conclusion Centrifuges are important tools for many different...

  8. General Transfection

    Type
    Protocol
    ...transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid...

  10. Lentivirus Production

    Type
    Protocol
    ...transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid...

  11. Colony Formation Titering Assay

    Type
    Protocol
    ...to each well (each well gets one dilution) Also include an untransduced well by using 150 μL of media in...

  13. Pipetting Protocol

    Type
    Protocol
    ... discard into a biohazardous waste container. Conclusion Pipetting accurately and precisely is important...

  16. Pouring LB Agar Plates

    Type
    Protocol
    ...with the date and the medium they will contain including the identity of the antibiotic. Pro-Tip We batch...
Showing: 21 - 38 of 38 results