We narrowed to 30 results for: FIE

...On average, the DNA fragment that needs to be amplified should be within 1-10 kB in size. The structure...

...chance of having a mixture of plasmids in your purified DNA. This protocol explains how to isolate a single...

...proceeding to your next intended step with the now gel purified DNA. Find more information about DNA quantification...

...technique is a critical skill for success in numerous fields such as microbiology, molecular biology or cell...

...Indirect ELISA Method Run an indirect ELISA against a purified antigen to demonstrate the expected antibody dose...

...the cells to take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce membrane...

...vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid Cut vector...

...produces a lawn of cells, which cannot be accurately quantified. Add 150 μL of a viral dilution to each well ...

...results when the plasmid is linearized. Treat the purified AAV samples with DNase I to eliminate any contaminating...

.... You are now ready to purify your prep . The clarified supernatant can be kept overnight at 4 °C before...