We narrowed to 30 results for: FIE

1. Protocol - How to Design Primers

   Type

   Protocol

   ...On average, the DNA fragment that needs to be amplified should be within 1-10 kB in size. The structure...

2. Protocol - How to Streak a Plate

   Type

   Protocol

   ...chance of having a mixture of plasmids in your purified DNA. This protocol explains how to isolate a single...

3. Protocol - How to Purify DNA from an Agarose Gel

   Type

   Protocol

   ...proceeding to your next intended step with the now gel purified DNA. Find more information about DNA quantification...

4. Video Library

   Type

   Protocol

   ...technique is a critical skill for success in numerous fields such as microbiology, molecular biology or cell...

5. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

   Type

   Protocol

   ...Indirect ELISA Method Run an indirect ELISA against a purified antigen to demonstrate the expected antibody dose...

6. Protocol - Bacterial Transformation

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   Protocol

   ...the cells to take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce membrane...

7. Protocol - How to Ligate Plasmid DNA

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   Protocol

   ...vector - Checks viability of competent cells and verifies the antibiotic resistance of the plasmid Cut vector...

8. Colony Formation Titering Assay

   Type

   Protocol

   ...produces a lawn of cells, which cannot be accurately quantified. Add 150 μL of a viral dilution to each well ...

9. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ...results when the plasmid is linearized. Treat the purified AAV samples with DNase I to eliminate any contaminating...

10. AAV Production in HEK293 Cells

    Type

    Protocol

    .... You are now ready to purify your prep . The clarified supernatant can be kept overnight at 4 °C before...