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We narrowed to 39 results for: Ina;

Showing: 21 - 39 of 39 results

  1. Fluorescence Titering Assay

    Type
    Protocol
    ...FBS can be purchased already heat-inactivated, or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Polybrene (10mg/mL), Millipore TR-1003-G ...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C....

  2. General Transfection

    Type
    Protocol
    ...FBS can be purchased already head inactivated or it can be inactivated in the lab by heating to 56 °C for...glutamine such as glutaGRO, Corning 25-015-CI) Heat-inactivated FBS Low serum medium such as Opti-MEM or Opti-Pro...bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C....

  3. Pouring LB Agar Plates

    Type
    Protocol
    ...recommended temperature should kill most potential contaminants, certain types of spores will stubbornly survive...For example: If you’ll be preparing plates with a final concentration of 100 ug/mL ampicillin, you should...growth of only the resistant strain. If you get this final result, your plates are ready for use or storage...any temperature and should always check for contamination prior to use. Negative Result 1: Both Strains...

  4. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...by restriction digest of existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI... and CAGCT - 5' to the bottom oligo, making our final oligos 34 bp each: Top oligo: 5' - AATTC CATATG ...this would destroy the EcoRI and SalI sites in the final vector. Order the following oligos from your favorite...

  5. Weighing Reagents Protocol

    Type
    Protocol
    ... material you’re weighing and prevent cross-contamination with other substances. They also help you transfer...sterile (if necessary), to prevent any potential contamination. Scoop out the material you are weighing onto...much material, don’t put any extra back into the original container. Dispose of the excess material into...

  6. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...Background Information The final step in the construction of a recombinant plasmid is connecting the insert...vector Insert or water + Any colonies indicate contamination of intact plasmid in ligation or transformation...

  7. Gibson Assembly Protocol

    Type
    Protocol
    ...DNA segments you will need to join to create your final plasmid. Adjacent segments should have identical...digest . Sequence the important regions of your final plasmid, particularly the seams between the assembled...especially useful for introducing promoters, terminators, and other short sequences into the assembly ...

  8. Using a Light Microscope Protocol

    Type
    Protocol
    ...where the image is further magnified before it finally reaches your eyes. These lenses determine the magnification...lens provides 10x magnification. To determine the final magnification of your image, multiply the magnification...sample. To ensure that your sample is adequately illuminated, look through the eyepiece. Adjust the power ...

  9. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...Repeat steps 2-4 25-30 times. Final Extension for 5 minutes at 72°C: A final extension to fill-in any protruding...minutes at 72°C. Repeat steps 8-10 for 25-30 cycles. Final Extension for 5 minutes at 72°C. Run 2 μL on a gel...

  10. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...band to cut without having DNA in other lanes contaminating your sample. To accomplish this, it is best ...each buffer to add during the DNA isolation step. Finally, you will want to isolate the DNA from the gel....

  12. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...of the buffer will evaporate and thus alter the final percentage of agarose in the gel. Many people prefer...mins. Optional : Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (usually...

  14. Western Blot

    Type
    Protocol
    ... Antibody Guide Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Introduction Western...Assay Kit or other preferred method for protein determination. Prepare a 50:1 Reagent A to Reagent B dilution...

  15. Kit Free RNA Extraction

    Type
    Protocol
    ...equipment, and reagents RNase-free (Use an RNase decontamination solution, such as RNaseZap® or RNase AWAY®,...microcentrifuge tubes, 4 mL polypropylene tubes RNase decontamination solution like RNase AWAY® or RNaseZAP® Isopropanol...

  16. Protocol - How to Design Primers

    Type
    Protocol
    ... primers) or too short. Short primers produce inaccurate, nonspecific DNA amplification product, and long...

  19. Immunocytochemistry

    Type
    Protocol
    ...Blog Posts Antibody Guide Western Blot Protocol Recombinant Antibody Purification Protocol Introduction Immunocytochemistry...
Showing: 21 - 39 of 39 results