...reagents to room temperature. Procedure Section 1: Prepare the Antigen Standard and coat the plate Dilute ... antigen to determine the ideal concentration. Prepare serial dilutions of the purified antigen as follows...overnight at 4 °C . Section 2: Block the plate Prepare the wash buffer (0.05% Tween-20 in PBS) as follows...wash, aspirate the wash buffer from the wells. Prepare the blocking buffer (1% BSA in PBS) as follows:...wash, aspirate the wash buffer from the wells. Prepare antibody dilution buffer (0.1% BSA in PBS) as follows...

...conditioned medium prepared above to make a new cell solution at a concentration of 5 cells/mL. Prepare approximately...culture dish, Corning 430167 (optional) Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM L-alanyl-...a hemocytometer or other cell counter. Pro-Tip Prepare a relatively dilute cell solution (<10 6 cells/.... If you choose not to use conditioned medium, prepare this cell solution in fresh complete medium. This...

...Purification Purify adeno-associated virus from a preparation produced in mammalian cells AAV Purification ... Inoculating a Liquid Bacterial Culture How to prepare and grow bacteria in a liquid medium Inoculating...physical titer of your adeno-associated virus preparation. AAV Titration by qPCR Using SYBR Green Technology...

... (PES) filter Syringes for filtering Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM L-alanyl-...empirically determined for each new batch of 1 mg/mL PEI prepared. There may be variation between batches of PEI...empirically determined. Once a batch of PEI is prepared, transfect cells with a fluorescent plasmid using...

...primary antibodies raised in a mouse. Reagent Preparation Permeabilization buffer: Dilute 20 µL of Triton...100 in 50 mL PBS. 300 nM DAPI working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1 µL...DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution by diluting 5 µL...

...plasmid encoding your insert of interest Reagent Preparation DMEM Complete (also known as D10): 10% v/v FBS...Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Prepare a mixture of the three transfection plasmids into...empirically determined. Once a batch of PEI is prepared, transfect cells with a fluorescent plasmid using...

...their resistance genes, as one does not need to prepare separate batches of antibiotic-containing agar....or other antibiotic resistance) Procedure Day 1 Prepare carbenicillin to a concentration of 1 mg/mL – 4...

...many different experimental procedures, such as minipreps and RNA extractions. Plus, centrifuges are useful...

...sell competent cells, which come frozen and are prepared for optimal transformation efficiencies upon thawing...

...image of that object! Equipment Light Microscope Prepared Slides (or other sample that can fit on a microscope...

...and concentration of PCR product. Master Mix Preparation Multiply the volume of each reagent by the number...

...to room temperature (RT) before use. Reagent Preparation 1 M sodium phosphate monobasic monohydrate (NaH...

...Protocol Before starting this protocol, make sure to prepare solution D (see reagent section above for the recipe...