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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 37 results for: cha;

Showing: 21 - 37 of 37 results

  1. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... blue mix into a cell counting chamber. Load the cell counting chamber on an automated cell counter and...conical tubes Microcentrifuge tubes Cell counting chamber slide 30 mL luer-lock syringe, BD BD302832 0.2 ...

  2. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...uses an electrical field to move the negatively charged DNA through an agarose gel matrix toward a positive...some to the buffer as well. EtBr is positively charged and will run the opposite direction from the DNA...negative, red is positive. The DNA is negatively charged and will run towards the positive electrode. Always...

  3. CRISPR Library Amplification

    Type
    Protocol
    ...Quality Control (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but...can be used but this dramatically increases the chances of individual plasmids being lost from the pool...taken to perform adequate NGS based QC to ensure no change in representation compared to the pre-amplified...

  4. Video Library

    Type
    Protocol
    ...Iodixanol Gradient Ultracentrifugation Protocol Mulitchannel Pipetting Technique Learn how to pipette multiple...multiple samples at once using a multichannel pipette and avoid inconsistent pipetting with our simple ...

  5. Centrifugation

    Type
    Protocol
    ...directly interchangeable. If you need to convert between the two, use a conversion calculator or chart like...

  6. Gibson Assembly Protocol

    Type
    Protocol
    ...two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations). Generate...or follow manufacturer's instructions. You can purchase master mix or make your own. Transform bacteria...

  7. Protocol - How to Design Primers

    Type
    Protocol
    ...Design a Primer You may also like... Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis...

  13. Ligation Independent Cloning

    Type
    Protocol
    ...concentrations of insert, thereby increasing your chances of success. Set up a vector only control with water...
Showing: 21 - 37 of 37 results