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Showing: 21 - 39 of 39 results

  1. Western Blot

    Type
    Protocol
    ...shaking platform. Dilute the horseradish peroxidase-conjugated secondary antibody to the desired concentration...transfected with the protein of interest, and a negative control sample that does not express the protein...

  2. AAV ddPCR Titration

    Type
    Protocol
    ...CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT ITR Probe: -FAM-CACTCCCTCTCTGCGCGCTCG-BBQ...data there should be a clear distinction between negative droplets (black) and positive droplets (blue)....

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...carefully bring a centrifuge into a cold room for centrifugation. Once you’re done using the centrifuge, bring...

  4. Protocol - How to Design Primers

    Type
    Protocol
    ...corresponds completely to the template DNA strand so elongation can proceed. Usually a guanine or cytosine is...

  7. Weighing Reagents Protocol

    Type
    Protocol
    ...reagents, determine the amount that you need to weigh. Gather the reagent you will be weighing out, a weighing...

  10. Video Library

    Type
    Protocol
    ...cells AAV Purification by Iodixanol Gradient Ultracentrifugation Protocol Mulitchannel Pipetting Technique...

  12. Gibson Assembly Protocol

    Type
    Protocol
    ...enzymes, the product of a Gibson Assembly is a fully ligated double-stranded DNA molecule. This has proven to...

  13. Immunocytochemistry

    Type
    Protocol
    ...plasmid to express the protein of interest, and a negative control sample such as cells that do not express...

  14. Pipetting Protocol

    Type
    Protocol
    ... changes to the amount you are dispensing can negatively impact your experimental results. This protocol...

  18. AAV Production in HEK293 Cells

    Type
    Protocol
    ...stained with Trypan Blue. Pellet cell debris by centrifugation at 3900 rpm for 10 min at 4 °C. Transfer the...

  19. CRISPR Library Amplification

    Type
    Protocol
    ...containing only the elements required for bacterial propagation (origin of replication and antibiotic selection...
Showing: 21 - 39 of 39 results