We narrowed to 27 results for: vent

1. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

   Type

   Protocol

   ...digestion, you will need to use a phosphatase to prevent re-circularization of the recipient plasmid. You...

2. What is Polymerase Chain Reaction (PCR)

   Type

   Protocol

   ...fill the remaining 50 μL of the reaction mix. Its solvent and buffer capabilities provide a suitable environment...

3. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...digestion, you will need to use a phosphatase to prevent re-circularization of the vector. You should treat...

4. Antibody Validation Using the Indirect ELISA Method

   Type

   Protocol

   ...available. Caution Use the Stop Solution in a well ventilated area and avoid breathing in the vapors. Workflow...

5. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

   ...population that varies in the number of integration events and the site(s) of proviral integration across ...

6. AAV Production in HEK293 Cells

   Type

   Protocol

   ... time. Allow them to mix and recheck the pH to prevent over or undershooting the desired pH. Allow the...

7. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...immediately or aliquot the media into smaller tubes to prevent multiple freeze/thaw cycles. Back to Top F. Infecting...