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TypeProtocol...tight. Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains...grow for longer periods of time (approximately 18-30 hr). On the other hand, smaller plasmids can be present...number plasmids should only need to be grown for 12-16 hr on average. Certain features of a plasmid may render...