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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17 results for: Rel;

Showing: 1 - 17 of 17 results

  1. Protocol - How to Design Primers

    Type
    Protocol
    ...in size. The structure of the primer should be relatively simple and contain no internal secondary structure...

  2. Water Bath Protocol

    Type
    Protocol
    ... your experiment. Water baths tend to heat up relatively slowly. Place the water bath cover on the top...

  4. Centrifugation

    Type
    Protocol
    ...protocol. Pro-Tip Spin speed is often given as Relative Centrifugal Force (RCF) or Revolutions Per Minute...

  5. Lentivirus ddPCR Titration

    Type
    Protocol
    ... across the dilutions. RPP30 copies should be relatively constant across samples. In the RRE example below...concentration of RPP30 positive droplets stays relatively even across samples (green). To increase the ...

  6. Protocol - Bacterial Transformation

    Type
    Protocol
    ...also make their own competent cells. This is a relatively simple procedure and is useful for performing...idea to use electro-competent cells. Instead of relying on the heat-shock to cause the cells to take up...

  7. Pipetting Protocol

    Type
    Protocol
    ...be in the liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid...samples. Tips can come loose in a bag, or can come preloaded into tip boxes. In either case, once assembled.... Once the end of the tip is submerged, slowly release the plunger, allowing it to go back to its resting... the plunger all the way to the second stop to release the liquid. Pro-Tip Dispense until the first stop... expel any residual liquid in the tip. Before releasing the plunger, lift the pipette tip out of the liquid...Once you are clear of the liquid you can slowly release the plunger. Remove the entire pipette from the...

  8. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...be 10 µg/mL. Pro-Tip Transducing too many cells relative to the number of virus particles reduces the transduction...antibiotic in culture, or remove the antibiotic entirely. If the antibiotic is reduced or removed from ...

  11. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B...TRC cloning vector has a 1.9kb stuffer that is released by digestion with AgeI and EcoRI. shRNA oligos...terminal repeat. RRE Rev response element. A.3 Related Products The following plasmids available from ... have at least 3 nucleotide mismatches to all unrelated genes. TIP: Addgene recommends that you select...cloning vector contains a 1.9kb stuffer that is released upon digestion with EcoRI and AgeI. The oligos...

  12. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...reference (typically ROX dye), to normalize non-PCR–related fluorescence fluctuations and to minimize well-...tested. Plasmid #59462 is one plasmid that gave reliable and consistent results. Use the recommended plasmid...following: Standard curve: R 2 (coefficient of correlation) ~ 1.0, E (efficiency of PCR) ~100% (90%–110%...

  13. CRISPR Library Amplification

    Type
    Protocol
    ...purification. Immediately purify them! Commercial Maxipreps rely on incremental, ordered cell lysis. E. coli cells...digest. Recombination is thought to be in part related to selective pressure. These recombined plasmids...can dramatically plateau and sometimes fall off entirely if the column or reagents are significantly overloaded...

  14. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...beginning or end of a sequencing reaction is often unreliable. Although your sequencing results may indicate... file, you will see that these base calls are unreliable. This is an example of a trace file from a portion...

  15. Video Library

    Type
    Protocol
    ... opens in a new window) Video Link Description Related Page Making LB Agar Plates Create plates to culture... opens in a new window) Video Link Description Related Page MTA Guide - Requests Your guide to the MTA...
Showing: 1 - 17 of 17 results