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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14 results for: Rel;

Showing: 1 - 14 of 14 results

  1. Antibody Guide

    Type
    Guide
    ...colored product that accumulates over time which is relative to the amount of enzyme present in each well. ... categories: Quantification - used to quantify relative amounts of protein. Includes: Western blot ELISA... western blots or ELISAs, are used to quantify relative amounts of protein. Western blot Western blots...proteins separated by size on an SDS-PAGE gel. Though relative quantification can be done, western blots primarily...referred to as housekeeping genes). This allows for relative comparison of expression between different samples... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv - Single-chain...

  2. Cloning

    Type
    Guide
    ...hundreds of available enzymes, many of which are relatively cheap. They also cut specific target sequences...and choice of restriction site. Because of the relatively long stretches of base pairing in the annealed...called TOPO® cloning or TA cloning, is a method that relies on the hybridization of the complementary base ...Independent Cloning Ligation Independent Cloning (LIC) relies on the 3'-5' exonuclease activity of T4 DNA polymerase...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...disease-associated genes. Gene Therapy Due to their relative safety and ability to transduce non-dividing cells...lentiviral gene therapies have been shown to be relatively safe and effective. Viral Vector Safety The two...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR in cis...HEK293T cells will produce the viral vectors and release them into the supernatant. After an initial media...particularly effective for stem cell therapies, which rely on slowly proliferating cell populations that can...institution's best practices with regard to lentiviral-related research. See Addgene’s Biosafety Resource Guide....72.11.8463-8471.1998 PMID: 9765382 Durand, S., & Cimarelli, A. (2011). The inside out of lentiviral vectors...

  4. Molecular Biology Reference

    Type
    Guide
    ...practically this isolation is only feasible for relatively short DNA molecules. In order to isolate a particular...most plasmids are replicated in E. coli and are relatively small (∼3000 - 6000 basepairs) to enable easy...Because bacteria are easy to grow in a lab, divide relatively quickly, and exhibit exponential growth rates..., and thus the plasmid exists in a symbiotic relationship with the host cell. Like the bacterial chromosomal...recommend checking out these two Addgene blog posts relating to common E. coli lab strains and E. coli strains...specialized for protein expression for additional strain-related information and a more extensive strain list. Strain...endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega e14-(McrA-) recA1 endA1...

  5. Plan Your Experiment

    Type
    Guide
    ...enables researchers to manipulate the genome with relative ease. Over the past decade, researchers have expanded... edit through HDR. Base and prime editing are relatively inefficient methods and often require screening... Cas enzyme at the right spot within the genome relies on proper gRNA design. While there are some cases...antibiotic resistance or fluorophore) are also relevant. Delivery Using Plasmids The most straightforward...

  6. Adenovirus Guide

    Type
    Guide
    ... choice due to their well-established methods, relative simplicity of production, and higher titers. However...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer/Shuttle plasmid ...which is how they got their name, but they are not related. AAVs require the presence of adenoviral genes ... inside of the adenoviral capsid and directly correlates to the size of the DNA removed from the viral...vector production platform, which like AdEasy™, relies on the homologous recombination of the shuttle ... preferentially infect certain tissues, which correlates with the specific diseases they cause. Ad5 and...

  7. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...genes makes them suitable to be packaged in the relatively small rAAV capsids. Learn more about optogenetics...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer/Shuttle plasmid ...which is how they got their name, but they are not related. AAVs require the presence of adenoviral genes ...available inside of the small AAV capsid and directly correlates to the size of the DNA removed from the viral...institution's best practices with regard to AAV-related research. See Addgene’s Biosafety Resource Guide...produce new viruses, eventually lysing the cell to release them. Optogenetics Biological technique to control...

  8. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR in cis.... The HEK293T cells will produce the virus and release it into the supernatant. After an initial media...best practices with regard to gamma-retroviral-related research. See Addgene’s Biosafety Resource Guide...

  9. CRISPR Guide

    Type
    Guide
    ...insert donor DNA at “landing sites” defined by relatively short sequences. Some have been paired with CRISPR...high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template to accurately... transduced cell population, you will look for relevant hits using NGS technology. For more detail on ...As this reporter is degraded by Cas12a, it will release a quantifiable signal that indicates the presence... J., & Van Der Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively...

  10. Chemogenetics Guide

    Type
    Guide
    ...firing through stimulating phospholipase C, which releases intracellular calcium stores. There are currently... DREADDs in experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating..., Jin J (2015). The First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated...460. PMID: 15765260 (Link opens in a new window) Farrell MS, Pei Y, Wan Y, Yadav PN, Daigle TL, Urban DJ...

  11. Optogenetics Guide

    Type
    Guide
    ...the wild-type opsin of each and some examples of relevant variants. This list is not exhaustive - please...phototropin 1 LOVETRAP reversibly sequester and release proteins from cellular membranes using light. light-induced...light-controlled gene expression. These plasmids rely on the engineered two-component regulatory system...

  12. Educational Resources

    Type
    Guide
    ...any of our eBooks to learn about popular topics related to our collections. Titles include: Plasmids 101...Read about plasmids in Addgene’s repository , and related molecular biology techniques. Topics include: Genome...

  13. Science Guides

    Type
    Guide
    ...start learning about various scientific concepts related to our plasmid collection. Find links to the guides...

  14. Guide to Using Pooled Libraries

    Type
    Guide
    ...Both types of screen employ a selection method relevant to the phenotype being studied. Examples of published...
Showing: 1 - 14 of 14 results