We narrowed to 6 results for: atic

1. CRISPR Library Amplification

   Type

   Protocol

   ...cells) Less library DNA can be used but this dramatically increases the chances of individual plasmids...: Do not overload the Maxipreps as yield can dramatically plateau and sometimes fall off entirely if the...

2. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

   Type

   Protocol

   ...added when ordering them or can be performed enzymatically later. Experimental Procedure Digest and purify...

3. Gibson Assembly Protocol

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   Protocol

   ...RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA molecules up to several hundred...

4. Using a Light Microscope Protocol

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   Protocol

   ...started in the lab! Introduction Microscopes are emblematic of biological research and are found in many ...

5. Handling Plasmids from Addgene - Purifying Plasmid DNA

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   Protocol

   ...for 5 min. Note: Ribonuclease A (RNase A) is a pancreatic ribonuclease that digests single-stranded RNA...

6. What is Polymerase Chain Reaction (PCR)

   Type

   Protocol

   ...polymerase from the thermophilic bacterium, Thermus aquaticus (Taq) . Taq polymerase can withstand many heating...