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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 318 results for: atic

Showing: 1 - 20 of 318 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    ... series, we will describe the importance of bioinformatics at Addgene and how our search for solutions...Labs.              DNA sequencing drives our bioinformatics needs       Fig. 1: QC'ing data at Addgene...presented with a new challenge: automating the bioinformatics steps needed to transform the raw sequencing... for our QC team to analyze. Criteria for a bioinformatics workflow management system As novices to creating...entire ecosystem Proven performance with other bioinformatics purposes  Integrated user interface, so our... entire pipeline. Benefits of Nextflow for bioinformatics pipelining In many use-cases, including ours... system for scientific pipelines, including bioinformatics. We loved the parallels between Seqera’s mission...

  3. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...Traditional lab notebooks just won't cut it for bioinformatics. All kinds of biologists are finding themselves...prevent you from getting lost in the wide word of informatics. I am a molecular biologist who began coding ... one way to keep a virtual lab notebook for bioinformatics. In fact, there are endless ways and everyone... bioinformatic... bioinformatic analyses...

  4. CRISPR Library Amplification

    Type
    Protocol
    ...cells) Less library DNA can be used but this dramatically increases the chances of individual plasmids...: Do not overload the Maxipreps as yield can dramatically plateau and sometimes fall off entirely if the...

  6. Gibson Assembly Protocol

    Type
    Protocol
    ...RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly of DNA molecules up to several hundred...

  10. Plasmids for Stem Cell Research

    Type
    Collection
    ...iPSCs or transdifferentiation of a somatic cell to a new type of somatic cell. Sort the table by cell type...reprogramming of adult pancreatic exocrine cells to beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells...body. Multipotent stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell.... To create iPSCs, fully-differentiated adult somatic cells are returned to a pluripotent state using...they can be directly differentiated into specific somatic stem cells or fully differentiated cell types. ...possible to directly differentiate one differentiated somatic cell into another via transdifferentiation. This...Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Dec 21;318(5858):1917-20. ...

  11. CRISPR Guide

    Type
    Collection
    ...a wide spectrum of single-nucleotide variants (somatic hypermutation) in a larger editing window. These...recommended in contexts where such mutations would be problematic. Browse Plasmids: Base Edit CRISPR Prime Editing... Cas nucleases dCas9 Nuclease dead Cas9, an enzymatically inactive form of Cas9; can bind, but cannot ...2016). Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nature Methods...Bintu, L., Bhatt, A. S., & Hsu, P. D. (2022). Systematic discovery of recombinases for efficient integration...

  12. NETRF

    Type
    Collection
    ... and more effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers. The NETRF...States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  13. AAV for Neuronal Tracing

    Type
    Collection
    ...from Addgene's viral service encoding tools for monosynpatic retrograde neuronal tracing, to be used with...cells engineered to express TVA will be infected. Schematic depicting steps for using AAV and modified rabies...

  14. CRISPR Guide

    Type
    Guide
    ...a wide spectrum of single-nucleotide variants (somatic hypermutation) in a larger editing window. These...recommended in contexts where such mutations would be problematic. Browse Plasmids: Base Edit CRISPR Prime Editing... Cas nucleases dCas9 Nuclease dead Cas9, an enzymatically inactive form of Cas9; can bind, but cannot ...2016). Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nature Methods...Bintu, L., Bhatt, A. S., & Hsu, P. D. (2022). Systematic discovery of recombinases for efficient integration...

  15. CRISPR Plasmids - Tagging

    Type
    Collection
    ...System Geraldine Seydoux's lab has developed a systematic and scalable method to create marker-free mutations...markers or footprints, and can be scaled up for systematic editing of multiple genes. Plasmids can be found...

  16. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...transdifferentiated in vivo to pancreatic β-like-cells with the expression of pancreatic and duodenal homoeobox...discuss the two major processes by which an adult somatic cell is converted to a different cell type for ...conversion happens via: ReprogrammingA differentiated somatic cell reverts back to a pluripotent state, which...Transdifferentiation In this process, differentiated adult somatic cells are converted to cells of a different lineage...stages: Dedifferentiation - Conversion of adult somatic cells into the pluripotent state. Redifferentiation...KLF4, and C-MYC - that induce a differentiated somatic cell to revert back to a pluripotent stage (iPSC...factors can be delivered and expressed in multiple somatic cells via various methods. Some of the more common...

  17. Brain Initiative Collection

    Type
    Collection
    ...Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single... 107712-AAV9 pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single...

  18. Antibodies 101: ChIP

    Type
    Blog Post
    ...high-frequency sound waves to break up the DNA, or enzymatic digestion. Sonication provides a truly random ...but is harsher and requires more optimization. Enzymatic digestion, while gentler on the sample, introduces...While sonication is usually the preferred method, enzymatic digestion can be appropriate when your protein...factor or cofactor. In such cases, the gentler enzymatic digestion can preserve more of the protein:DNA... Capture After crosslinking and sonication or enzymatic digestion, you’re ready to incubate the sample...phenol-chloroform extraction.    Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via...could affect antibody selection. Sonication (or enzymatic digest) will also have to be optimized for both...

  19. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ... of Dr. Moshe Szyf at McGill University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone...which where they were absent before. But other enzymatic fusions, such as dCas9-TET, can, like the iron...tool of choice in the field: dCas9 fused to the enzymatic domain of TET1 or TET2 (Morita et al. 2016, Xu... transcriptional consequences (Sprujit 2013). Enzymatic domains tethered to dCas9 often interact with ...proteins affect transcription independent of their enzymatic activity. They interact with transcriptional machinery...counterintuitive: how can a DNA-binding protein with no enzymatic activity cause demethylation?  Dividing cells ...in pluripotent cells at thousands of methylated somatic enhancers. Nat Genet 52, 819–827. https://doi.org...

  20. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...-to-sequence plasmids, other sequencing and bioinformatic technologies are used.  Improvements to sequencing...sequencing technologies and bioinformatic analysis are occurring every year. At Addgene, we want to maintain...cassettes of the same gene multiple times.  Bioinformatics at Addgene After a run on our Illumina MiSeq...sequences submitted by depositors. A sample will automatically pass through our QC process if there is a 100%... of emerging technologies in sequencing and bioinformatic analysis. At Addgene, we use a mix-and-match... F. (2021). Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology, 39(...Addgene Blog A Look at Addgene’s QC Process Bioinformatics at Addgene The Power Behind NGS Plasmid Validation...
Showing: 1 - 20 of 318 results