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Showing: 121 - 140 of 3776 results

  2. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-90684

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  3. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  4. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  16. pAAV-FLEX-C-RG

    Plasmid
    #49101
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under EF1a promoter, in AAV virus
    Depositor
    Troy Margrie
    Insert
    Cerulean-E2A-RG
    Use
    AAV and Cre/Lox
    Tags
    cerulean
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    49101-plasmid-map-sequence-id-91148

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Showing: 121 - 140 of 3776 results